Experiment: R2A with Dimethyl Sulfoxide 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS04210 and RR42_RS04215 overlap by 11 nucleotides RR42_RS04215 and RR42_RS04220 are separated by 6 nucleotides RR42_RS04220 and RR42_RS04225 are separated by 15 nucleotides
RR42_RS04210: RR42_RS04210 - membrane protein, at 932,017 to 933,306
_RS04210
RR42_RS04215: RR42_RS04215 - hypothetical protein, at 933,296 to 933,799
_RS04215
RR42_RS04220: RR42_RS04220 - membrane protein, at 933,806 to 934,666
_RS04220
RR42_RS04225: RR42_RS04225 - hypothetical protein, at 934,682 to 935,302
_RS04225
Position (kb)
933
934
935 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 932.809 kb on + strand, within RR42_RS04210 at 932.809 kb on + strand, within RR42_RS04210 at 932.923 kb on + strand, within RR42_RS04210 at 932.923 kb on + strand, within RR42_RS04210 at 932.923 kb on + strand, within RR42_RS04210 at 932.923 kb on + strand, within RR42_RS04210 at 932.924 kb on - strand, within RR42_RS04210 at 932.924 kb on - strand, within RR42_RS04210 at 932.924 kb on - strand, within RR42_RS04210 at 932.924 kb on - strand, within RR42_RS04210 at 933.019 kb on + strand, within RR42_RS04210 at 933.020 kb on - strand, within RR42_RS04210 at 933.265 kb on + strand at 933.265 kb on + strand at 933.266 kb on - strand at 933.266 kb on - strand at 933.266 kb on - strand at 933.413 kb on + strand, within RR42_RS04215 at 933.445 kb on + strand, within RR42_RS04215 at 933.446 kb on - strand, within RR42_RS04215 at 933.446 kb on - strand, within RR42_RS04215 at 933.532 kb on + strand, within RR42_RS04215 at 933.532 kb on + strand, within RR42_RS04215 at 933.532 kb on + strand, within RR42_RS04215 at 933.532 kb on + strand, within RR42_RS04215 at 933.533 kb on - strand, within RR42_RS04215 at 933.533 kb on - strand, within RR42_RS04215 at 933.533 kb on - strand, within RR42_RS04215 at 933.533 kb on - strand, within RR42_RS04215 at 933.533 kb on - strand, within RR42_RS04215 at 933.534 kb on + strand, within RR42_RS04215 at 933.534 kb on + strand, within RR42_RS04215 at 933.534 kb on + strand, within RR42_RS04215 at 933.534 kb on + strand, within RR42_RS04215 at 933.535 kb on - strand, within RR42_RS04215 at 933.535 kb on - strand, within RR42_RS04215 at 933.535 kb on - strand, within RR42_RS04215 at 933.535 kb on - strand, within RR42_RS04215 at 933.535 kb on - strand, within RR42_RS04215 at 934.185 kb on + strand, within RR42_RS04220 at 934.186 kb on - strand, within RR42_RS04220 at 934.392 kb on + strand, within RR42_RS04220 at 934.392 kb on + strand, within RR42_RS04220 at 934.424 kb on + strand, within RR42_RS04220 at 934.464 kb on + strand, within RR42_RS04220 at 934.610 kb on + strand at 934.666 kb on + strand at 934.667 kb on - strand at 934.667 kb on - strand at 934.667 kb on - strand at 934.667 kb on - strand at 934.670 kb on + strand at 934.670 kb on + strand at 934.670 kb on + strand at 934.670 kb on + strand at 934.671 kb on - strand at 934.671 kb on - strand at 934.671 kb on - strand at 934.671 kb on - strand at 934.671 kb on - strand at 934.671 kb on - strand at 934.806 kb on + strand, within RR42_RS04225 at 934.807 kb on - strand, within RR42_RS04225 at 934.807 kb on - strand, within RR42_RS04225 at 934.826 kb on - strand, within RR42_RS04225 at 934.827 kb on + strand, within RR42_RS04225 at 934.827 kb on + strand, within RR42_RS04225 at 934.828 kb on - strand, within RR42_RS04225 at 934.828 kb on - strand, within RR42_RS04225 at 934.828 kb on - strand, within RR42_RS04225 at 934.828 kb on - strand, within RR42_RS04225 at 935.091 kb on + strand, within RR42_RS04225 at 935.092 kb on - strand, within RR42_RS04225 at 935.092 kb on - strand, within RR42_RS04225 at 935.105 kb on - strand, within RR42_RS04225 at 935.143 kb on - strand, within RR42_RS04225 at 935.205 kb on + strand, within RR42_RS04225 at 935.205 kb on + strand, within RR42_RS04225 at 935.258 kb on + strand at 935.258 kb on + strand at 935.335 kb on + strand at 935.336 kb on - strand at 935.336 kb on - strand at 935.362 kb on + strand at 935.363 kb on - strand at 935.613 kb on + strand at 935.613 kb on + strand at 935.614 kb on - strand at 935.614 kb on - strand at 935.614 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Dimethyl Sulfoxide 5 vol% remove 932,809 + RR42_RS04210 0.61 +1.2 932,809 + RR42_RS04210 0.61 +0.5 932,923 + RR42_RS04210 0.70 -0.6 932,923 + RR42_RS04210 0.70 -0.0 932,923 + RR42_RS04210 0.70 -0.1 932,923 + RR42_RS04210 0.70 -0.6 932,924 - RR42_RS04210 0.70 -0.3 932,924 - RR42_RS04210 0.70 +0.0 932,924 - RR42_RS04210 0.70 -0.1 932,924 - RR42_RS04210 0.70 -0.3 933,019 + RR42_RS04210 0.78 -1.4 933,020 - RR42_RS04210 0.78 -0.3 933,265 + -1.2 933,265 + -0.4 933,266 - -1.0 933,266 - +1.7 933,266 - +0.1 933,413 + RR42_RS04215 0.23 -1.2 933,445 + RR42_RS04215 0.30 +0.3 933,446 - RR42_RS04215 0.30 -0.3 933,446 - RR42_RS04215 0.30 -0.1 933,532 + RR42_RS04215 0.47 -1.5 933,532 + RR42_RS04215 0.47 +0.1 933,532 + RR42_RS04215 0.47 -1.0 933,532 + RR42_RS04215 0.47 -0.1 933,533 - RR42_RS04215 0.47 -0.6 933,533 - RR42_RS04215 0.47 +0.3 933,533 - RR42_RS04215 0.47 +0.0 933,533 - RR42_RS04215 0.47 -1.0 933,533 - RR42_RS04215 0.47 -0.7 933,534 + RR42_RS04215 0.47 -1.0 933,534 + RR42_RS04215 0.47 +0.1 933,534 + RR42_RS04215 0.47 -0.3 933,534 + RR42_RS04215 0.47 -1.6 933,535 - RR42_RS04215 0.47 -0.1 933,535 - RR42_RS04215 0.47 -1.0 933,535 - RR42_RS04215 0.47 -0.1 933,535 - RR42_RS04215 0.47 -1.4 933,535 - RR42_RS04215 0.47 -0.3 934,185 + RR42_RS04220 0.44 -3.1 934,186 - RR42_RS04220 0.44 -0.8 934,392 + RR42_RS04220 0.68 -0.0 934,392 + RR42_RS04220 0.68 -2.3 934,424 + RR42_RS04220 0.72 +1.7 934,464 + RR42_RS04220 0.76 -0.2 934,610 + -0.7 934,666 + +0.3 934,667 - -0.2 934,667 - -0.6 934,667 - -0.4 934,667 - +0.1 934,670 + +0.1 934,670 + -1.2 934,670 + -0.5 934,670 + -0.4 934,671 - -0.6 934,671 - -0.2 934,671 - +1.3 934,671 - -0.4 934,671 - +0.5 934,671 - +0.9 934,806 + RR42_RS04225 0.20 +1.0 934,807 - RR42_RS04225 0.20 +0.7 934,807 - RR42_RS04225 0.20 -0.3 934,826 - RR42_RS04225 0.23 -0.8 934,827 + RR42_RS04225 0.23 -0.2 934,827 + RR42_RS04225 0.23 +0.2 934,828 - RR42_RS04225 0.24 +0.2 934,828 - RR42_RS04225 0.24 +2.2 934,828 - RR42_RS04225 0.24 -0.1 934,828 - RR42_RS04225 0.24 +0.6 935,091 + RR42_RS04225 0.66 +0.5 935,092 - RR42_RS04225 0.66 -0.7 935,092 - RR42_RS04225 0.66 -0.5 935,105 - RR42_RS04225 0.68 -0.1 935,143 - RR42_RS04225 0.74 -1.1 935,205 + RR42_RS04225 0.84 +0.1 935,205 + RR42_RS04225 0.84 +0.1 935,258 + +0.7 935,258 + -2.7 935,335 + -0.8 935,336 - -0.5 935,336 - -1.0 935,362 + +1.7 935,363 - +0.6 935,613 + +0.3 935,613 + -0.3 935,614 - -0.8 935,614 - +0.0 935,614 - +1.8
Or see this region's nucleotide sequence