Experiment: R2A with Dimethyl Sulfoxide 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS04105 and RR42_RS04110 are separated by 25 nucleotides RR42_RS04110 and RR42_RS04115 overlap by 4 nucleotides RR42_RS04115 and RR42_RS04120 are separated by 9 nucleotides RR42_RS04120 and RR42_RS04125 are separated by 38 nucleotides RR42_RS04125 and RR42_RS04130 are separated by 34 nucleotides
RR42_RS04105: RR42_RS04105 - pilus assembly protein CpaE, at 908,648 to 909,841
_RS04105
RR42_RS04110: RR42_RS04110 - pilus assembly protein TadG, at 909,867 to 910,325
_RS04110
RR42_RS04115: RR42_RS04115 - pilus assembly protein TadG, at 910,322 to 910,831
_RS04115
RR42_RS04120: RR42_RS04120 - pilus assembly protein TadG, at 910,841 to 912,121
_RS04120
RR42_RS04125: RR42_RS04125 - lipoprotein, at 912,160 to 912,501
_RS04125
RR42_RS04130: RR42_RS04130 - secretin, at 912,536 to 914,383
_RS04130
Position (kb)
910
911
912
913 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 909.868 kb on + strand at 909.868 kb on + strand at 909.869 kb on - strand at 909.990 kb on + strand, within RR42_RS04110 at 910.196 kb on - strand, within RR42_RS04110 at 910.325 kb on + strand at 910.328 kb on + strand at 910.329 kb on - strand at 910.329 kb on - strand at 910.401 kb on + strand, within RR42_RS04115 at 910.401 kb on + strand, within RR42_RS04115 at 910.402 kb on - strand, within RR42_RS04115 at 910.405 kb on + strand, within RR42_RS04115 at 910.405 kb on + strand, within RR42_RS04115 at 910.406 kb on - strand, within RR42_RS04115 at 910.406 kb on - strand, within RR42_RS04115 at 910.600 kb on - strand, within RR42_RS04115 at 910.863 kb on - strand at 910.863 kb on - strand at 911.172 kb on + strand, within RR42_RS04120 at 911.219 kb on - strand, within RR42_RS04120 at 911.219 kb on - strand, within RR42_RS04120 at 911.496 kb on + strand, within RR42_RS04120 at 911.550 kb on + strand, within RR42_RS04120 at 911.550 kb on + strand, within RR42_RS04120 at 911.745 kb on + strand, within RR42_RS04120 at 911.746 kb on - strand, within RR42_RS04120 at 911.767 kb on - strand, within RR42_RS04120 at 911.814 kb on + strand, within RR42_RS04120 at 911.814 kb on + strand, within RR42_RS04120 at 911.873 kb on - strand, within RR42_RS04120 at 911.876 kb on + strand, within RR42_RS04120 at 912.260 kb on - strand, within RR42_RS04125 at 912.275 kb on + strand, within RR42_RS04125 at 912.276 kb on - strand, within RR42_RS04125 at 912.276 kb on - strand, within RR42_RS04125 at 912.729 kb on - strand, within RR42_RS04130 at 912.775 kb on + strand, within RR42_RS04130 at 912.900 kb on + strand, within RR42_RS04130 at 912.901 kb on - strand, within RR42_RS04130 at 912.922 kb on + strand, within RR42_RS04130 at 913.082 kb on + strand, within RR42_RS04130
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Dimethyl Sulfoxide 5 vol% remove 909,868 + +1.2 909,868 + -0.2 909,869 - -0.1 909,990 + RR42_RS04110 0.27 -0.7 910,196 - RR42_RS04110 0.72 -0.4 910,325 + +1.8 910,328 + +0.2 910,329 - -0.6 910,329 - -0.9 910,401 + RR42_RS04115 0.15 +1.3 910,401 + RR42_RS04115 0.15 -0.5 910,402 - RR42_RS04115 0.16 +0.1 910,405 + RR42_RS04115 0.16 -0.3 910,405 + RR42_RS04115 0.16 +3.5 910,406 - RR42_RS04115 0.16 +1.0 910,406 - RR42_RS04115 0.16 -0.1 910,600 - RR42_RS04115 0.55 -0.6 910,863 - +0.1 910,863 - +0.5 911,172 + RR42_RS04120 0.26 +0.8 911,219 - RR42_RS04120 0.30 -0.4 911,219 - RR42_RS04120 0.30 +0.2 911,496 + RR42_RS04120 0.51 -0.5 911,550 + RR42_RS04120 0.55 -1.4 911,550 + RR42_RS04120 0.55 -1.2 911,745 + RR42_RS04120 0.71 +1.0 911,746 - RR42_RS04120 0.71 -0.2 911,767 - RR42_RS04120 0.72 -0.5 911,814 + RR42_RS04120 0.76 +0.2 911,814 + RR42_RS04120 0.76 -0.7 911,873 - RR42_RS04120 0.81 -0.6 911,876 + RR42_RS04120 0.81 -1.3 912,260 - RR42_RS04125 0.29 -0.7 912,275 + RR42_RS04125 0.34 +2.4 912,276 - RR42_RS04125 0.34 -0.4 912,276 - RR42_RS04125 0.34 -0.4 912,729 - RR42_RS04130 0.10 -2.6 912,775 + RR42_RS04130 0.13 +0.5 912,900 + RR42_RS04130 0.20 +0.0 912,901 - RR42_RS04130 0.20 +0.3 912,922 + RR42_RS04130 0.21 +0.0 913,082 + RR42_RS04130 0.30 -0.7
Or see this region's nucleotide sequence