Experiment: R2A with Dimethyl Sulfoxide 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS03870 and RR42_RS03875 are separated by 166 nucleotides RR42_RS03875 and RR42_RS03880 are separated by 47 nucleotides RR42_RS03880 and RR42_RS03885 overlap by 1 nucleotides
RR42_RS03870: RR42_RS03870 - hypothetical protein, at 855,067 to 855,261
_RS03870
RR42_RS03875: RR42_RS03875 - nucleoside 2-deoxyribosyltransferase, at 855,428 to 855,976
_RS03875
RR42_RS03880: RR42_RS03880 - hypothetical protein, at 856,024 to 856,680
_RS03880
RR42_RS03885: RR42_RS03885 - mammalian cell entry protein, at 856,680 to 858,311
_RS03885
Position (kb) 856
857 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 855.080 kb on - strand at 855.080 kb on - strand at 855.081 kb on + strand at 855.440 kb on + strand at 855.440 kb on + strand at 855.440 kb on + strand at 855.515 kb on + strand, within RR42_RS03875 at 855.516 kb on - strand, within RR42_RS03875 at 856.014 kb on + strand at 856.298 kb on + strand, within RR42_RS03880 at 856.667 kb on - strand at 856.947 kb on + strand, within RR42_RS03885 at 857.129 kb on - strand, within RR42_RS03885 at 857.201 kb on + strand, within RR42_RS03885 at 857.260 kb on + strand, within RR42_RS03885 at 857.261 kb on - strand, within RR42_RS03885 at 857.309 kb on - strand, within RR42_RS03885 at 857.338 kb on - strand, within RR42_RS03885
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Dimethyl Sulfoxide 5 vol% remove 855,080 - +0.1 855,080 - -0.3 855,081 + -0.2 855,440 + -1.1 855,440 + -0.1 855,440 + -0.9 855,515 + RR42_RS03875 0.16 -0.0 855,516 - RR42_RS03875 0.16 -0.8 856,014 + -0.7 856,298 + RR42_RS03880 0.42 +0.8 856,667 - -0.0 856,947 + RR42_RS03885 0.16 -3.2 857,129 - RR42_RS03885 0.28 -0.4 857,201 + RR42_RS03885 0.32 +0.5 857,260 + RR42_RS03885 0.36 +0.8 857,261 - RR42_RS03885 0.36 -0.5 857,309 - RR42_RS03885 0.39 +1.5 857,338 - RR42_RS03885 0.40 -0.4
Or see this region's nucleotide sequence