Experiment: R2A with Dimethyl Sulfoxide 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS03670 and RR42_RS03675 are separated by 594 nucleotides RR42_RS03675 and RR42_RS03680 are separated by 27 nucleotides RR42_RS03680 and RR42_RS03685 are separated by 110 nucleotides RR42_RS03685 and RR42_RS03690 are separated by 579 nucleotides
RR42_RS03670: RR42_RS03670 - isochorismatase, at 812,619 to 812,813
_RS03670
RR42_RS03675: RR42_RS03675 - hypothetical protein, at 813,408 to 813,608
_RS03675
RR42_RS03680: RR42_RS03680 - hypothetical protein, at 813,636 to 813,878
_RS03680
RR42_RS03685: RR42_RS03685 - GTP cyclohydrolase, at 813,989 to 814,258
_RS03685
RR42_RS03690: RR42_RS03690 - hypothetical protein, at 814,838 to 815,059
_RS03690
Position (kb) 813
814 Strain fitness (log2 ratio) -2
-1
0
1
2 at 812.676 kb on - strand, within RR42_RS03670 at 812.764 kb on + strand, within RR42_RS03670 at 812.765 kb on - strand, within RR42_RS03670 at 812.796 kb on + strand at 812.797 kb on - strand at 812.818 kb on + strand at 812.818 kb on + strand at 812.850 kb on - strand at 812.858 kb on + strand at 812.859 kb on - strand at 813.017 kb on + strand at 813.017 kb on + strand at 813.063 kb on + strand at 813.064 kb on - strand at 813.167 kb on + strand at 813.168 kb on - strand at 813.244 kb on + strand at 813.244 kb on + strand at 813.286 kb on + strand at 813.286 kb on + strand at 813.287 kb on - strand at 813.295 kb on + strand at 813.295 kb on + strand at 813.296 kb on - strand at 813.296 kb on - strand at 813.296 kb on - strand at 813.438 kb on + strand, within RR42_RS03675 at 813.439 kb on - strand, within RR42_RS03675 at 813.439 kb on - strand, within RR42_RS03675 at 813.490 kb on - strand, within RR42_RS03675 at 813.490 kb on - strand, within RR42_RS03675 at 813.490 kb on - strand, within RR42_RS03675 at 813.492 kb on - strand, within RR42_RS03675 at 813.492 kb on - strand, within RR42_RS03675 at 813.500 kb on + strand, within RR42_RS03675 at 813.638 kb on - strand at 813.661 kb on + strand, within RR42_RS03680 at 813.661 kb on + strand, within RR42_RS03680 at 813.662 kb on - strand, within RR42_RS03680 at 813.662 kb on - strand, within RR42_RS03680 at 813.990 kb on + strand at 813.990 kb on + strand at 814.039 kb on - strand, within RR42_RS03685 at 814.208 kb on + strand, within RR42_RS03685 at 814.284 kb on + strand at 814.284 kb on + strand at 814.285 kb on - strand at 814.398 kb on + strand at 814.427 kb on - strand at 814.499 kb on - strand at 814.617 kb on + strand at 814.618 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Dimethyl Sulfoxide 5 vol% remove 812,676 - RR42_RS03670 0.29 -1.1 812,764 + RR42_RS03670 0.74 -2.1 812,765 - RR42_RS03670 0.75 -0.6 812,796 + +1.9 812,797 - +0.6 812,818 + +0.4 812,818 + +0.4 812,850 - -0.8 812,858 + +0.4 812,859 - -0.5 813,017 + +1.3 813,017 + +0.4 813,063 + -0.6 813,064 - -0.6 813,167 + -0.2 813,168 - -0.2 813,244 + +0.8 813,244 + +0.2 813,286 + +0.4 813,286 + +0.1 813,287 - -0.6 813,295 + +0.6 813,295 + +0.5 813,296 - +0.5 813,296 - -1.2 813,296 - +0.1 813,438 + RR42_RS03675 0.15 +0.7 813,439 - RR42_RS03675 0.15 -0.7 813,439 - RR42_RS03675 0.15 -0.1 813,490 - RR42_RS03675 0.41 -1.3 813,490 - RR42_RS03675 0.41 +0.9 813,490 - RR42_RS03675 0.41 -0.3 813,492 - RR42_RS03675 0.42 +0.5 813,492 - RR42_RS03675 0.42 -0.3 813,500 + RR42_RS03675 0.46 +0.3 813,638 - +0.9 813,661 + RR42_RS03680 0.10 +0.1 813,661 + RR42_RS03680 0.10 +0.6 813,662 - RR42_RS03680 0.11 +0.6 813,662 - RR42_RS03680 0.11 -1.4 813,990 + +0.5 813,990 + +0.1 814,039 - RR42_RS03685 0.19 +0.3 814,208 + RR42_RS03685 0.81 +0.7 814,284 + +1.3 814,284 + -0.3 814,285 - +0.2 814,398 + +0.8 814,427 - -0.2 814,499 - +0.3 814,617 + -0.2 814,618 - -0.1
Or see this region's nucleotide sequence