Experiment: R2A with Dimethyl Sulfoxide 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS03650 and RR42_RS03655 are separated by 215 nucleotides RR42_RS03655 and RR42_RS03660 are separated by 66 nucleotides RR42_RS03660 and RR42_RS03665 are separated by 80 nucleotides RR42_RS03665 and RR42_RS03670 are separated by 74 nucleotides RR42_RS03670 and RR42_RS03675 are separated by 594 nucleotides
RR42_RS03650: RR42_RS03650 - hypothetical protein, at 811,047 to 811,331
_RS03650
RR42_RS03655: RR42_RS03655 - hypothetical protein, at 811,547 to 811,921
_RS03655
RR42_RS03660: RR42_RS03660 - translation initiation factor 1, at 811,988 to 812,242
_RS03660
RR42_RS03665: RR42_RS03665 - translation initiation factor IF-1, at 812,323 to 812,544
_RS03665
RR42_RS03670: RR42_RS03670 - isochorismatase, at 812,619 to 812,813
_RS03670
RR42_RS03675: RR42_RS03675 - hypothetical protein, at 813,408 to 813,608
_RS03675
Position (kb) 812
813 Strain fitness (log2 ratio) -2
-1
0
1
2 at 811.329 kb on + strand at 811.330 kb on - strand at 811.330 kb on - strand at 811.359 kb on - strand at 811.415 kb on - strand at 811.495 kb on - strand at 811.541 kb on + strand at 811.542 kb on - strand at 811.542 kb on - strand at 811.542 kb on - strand at 811.542 kb on - strand at 811.542 kb on - strand at 811.614 kb on - strand, within RR42_RS03655 at 811.620 kb on + strand, within RR42_RS03655 at 811.620 kb on + strand, within RR42_RS03655 at 811.620 kb on + strand, within RR42_RS03655 at 811.621 kb on - strand, within RR42_RS03655 at 811.621 kb on - strand, within RR42_RS03655 at 811.621 kb on - strand, within RR42_RS03655 at 811.799 kb on + strand, within RR42_RS03655 at 811.800 kb on - strand, within RR42_RS03655 at 811.823 kb on + strand, within RR42_RS03655 at 811.824 kb on - strand, within RR42_RS03655 at 811.989 kb on + strand at 811.990 kb on - strand at 812.094 kb on + strand, within RR42_RS03660 at 812.095 kb on - strand, within RR42_RS03660 at 812.105 kb on + strand, within RR42_RS03660 at 812.105 kb on + strand, within RR42_RS03660 at 812.105 kb on + strand, within RR42_RS03660 at 812.106 kb on - strand, within RR42_RS03660 at 812.106 kb on - strand, within RR42_RS03660 at 812.106 kb on - strand, within RR42_RS03660 at 812.106 kb on - strand, within RR42_RS03660 at 812.122 kb on + strand, within RR42_RS03660 at 812.123 kb on - strand, within RR42_RS03660 at 812.189 kb on + strand, within RR42_RS03660 at 812.301 kb on + strand at 812.322 kb on - strand at 812.327 kb on + strand at 812.328 kb on - strand at 812.328 kb on - strand at 812.367 kb on - strand, within RR42_RS03665 at 812.441 kb on + strand, within RR42_RS03665 at 812.498 kb on - strand, within RR42_RS03665 at 812.527 kb on + strand at 812.539 kb on - strand at 812.676 kb on - strand, within RR42_RS03670 at 812.764 kb on + strand, within RR42_RS03670 at 812.765 kb on - strand, within RR42_RS03670 at 812.796 kb on + strand at 812.797 kb on - strand at 812.818 kb on + strand at 812.818 kb on + strand at 812.850 kb on - strand at 812.858 kb on + strand at 812.859 kb on - strand at 813.017 kb on + strand at 813.017 kb on + strand at 813.063 kb on + strand at 813.064 kb on - strand at 813.167 kb on + strand at 813.168 kb on - strand at 813.244 kb on + strand at 813.244 kb on + strand at 813.286 kb on + strand at 813.286 kb on + strand at 813.287 kb on - strand at 813.295 kb on + strand at 813.295 kb on + strand at 813.296 kb on - strand at 813.296 kb on - strand at 813.296 kb on - strand at 813.438 kb on + strand, within RR42_RS03675 at 813.439 kb on - strand, within RR42_RS03675 at 813.439 kb on - strand, within RR42_RS03675 at 813.490 kb on - strand, within RR42_RS03675 at 813.490 kb on - strand, within RR42_RS03675 at 813.490 kb on - strand, within RR42_RS03675 at 813.492 kb on - strand, within RR42_RS03675 at 813.492 kb on - strand, within RR42_RS03675 at 813.500 kb on + strand, within RR42_RS03675
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Dimethyl Sulfoxide 5 vol% remove 811,329 + -0.5 811,330 - +1.3 811,330 - -1.1 811,359 - +0.2 811,415 - -1.0 811,495 - +0.6 811,541 + -0.0 811,542 - +0.9 811,542 - +1.3 811,542 - -0.2 811,542 - -0.2 811,542 - -0.2 811,614 - RR42_RS03655 0.18 -1.2 811,620 + RR42_RS03655 0.19 +0.1 811,620 + RR42_RS03655 0.19 +0.1 811,620 + RR42_RS03655 0.19 -0.1 811,621 - RR42_RS03655 0.20 +0.5 811,621 - RR42_RS03655 0.20 -2.0 811,621 - RR42_RS03655 0.20 -0.5 811,799 + RR42_RS03655 0.67 +0.4 811,800 - RR42_RS03655 0.67 +0.0 811,823 + RR42_RS03655 0.74 +0.5 811,824 - RR42_RS03655 0.74 +0.0 811,989 + -1.0 811,990 - +0.9 812,094 + RR42_RS03660 0.42 +1.6 812,095 - RR42_RS03660 0.42 -0.0 812,105 + RR42_RS03660 0.46 +1.4 812,105 + RR42_RS03660 0.46 -0.6 812,105 + RR42_RS03660 0.46 -1.0 812,106 - RR42_RS03660 0.46 +0.1 812,106 - RR42_RS03660 0.46 +0.2 812,106 - RR42_RS03660 0.46 -0.9 812,106 - RR42_RS03660 0.46 +1.3 812,122 + RR42_RS03660 0.53 +2.1 812,123 - RR42_RS03660 0.53 +0.6 812,189 + RR42_RS03660 0.79 -0.4 812,301 + -0.4 812,322 - -0.2 812,327 + +0.6 812,328 - -0.1 812,328 - +0.7 812,367 - RR42_RS03665 0.20 +0.0 812,441 + RR42_RS03665 0.53 +0.7 812,498 - RR42_RS03665 0.79 +0.4 812,527 + -0.0 812,539 - -0.3 812,676 - RR42_RS03670 0.29 -1.1 812,764 + RR42_RS03670 0.74 -2.1 812,765 - RR42_RS03670 0.75 -0.6 812,796 + +1.9 812,797 - +0.6 812,818 + +0.4 812,818 + +0.4 812,850 - -0.8 812,858 + +0.4 812,859 - -0.5 813,017 + +1.3 813,017 + +0.4 813,063 + -0.6 813,064 - -0.6 813,167 + -0.2 813,168 - -0.2 813,244 + +0.8 813,244 + +0.2 813,286 + +0.4 813,286 + +0.1 813,287 - -0.6 813,295 + +0.6 813,295 + +0.5 813,296 - +0.5 813,296 - -1.2 813,296 - +0.1 813,438 + RR42_RS03675 0.15 +0.7 813,439 - RR42_RS03675 0.15 -0.7 813,439 - RR42_RS03675 0.15 -0.1 813,490 - RR42_RS03675 0.41 -1.3 813,490 - RR42_RS03675 0.41 +0.9 813,490 - RR42_RS03675 0.41 -0.3 813,492 - RR42_RS03675 0.42 +0.5 813,492 - RR42_RS03675 0.42 -0.3 813,500 + RR42_RS03675 0.46 +0.3
Or see this region's nucleotide sequence