Experiment: R2A with Dimethyl Sulfoxide 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS03335 and RR42_RS03340 are separated by 80 nucleotides RR42_RS03340 and RR42_RS03345 are separated by 35 nucleotides RR42_RS03345 and RR42_RS03350 are separated by 125 nucleotides RR42_RS03350 and RR42_RS03355 are separated by 245 nucleotides
RR42_RS03335: RR42_RS03335 - glycerol-3-phosphate acyltransferase, at 742,317 to 742,934
_RS03335
RR42_RS03340: RR42_RS03340 - membrane protein, at 743,015 to 743,506
_RS03340
RR42_RS03345: RR42_RS03345 - recombinase XerD, at 743,542 to 744,462
_RS03345
RR42_RS03350: RR42_RS03350 - cysteine methyltransferase, at 744,588 to 745,067
_RS03350
RR42_RS03355: RR42_RS03355 - N-acylmannosamine kinase, at 745,313 to 746,572
_RS03355
Position (kb) 743
744
745 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 743.016 kb on + strand at 743.017 kb on - strand at 743.017 kb on - strand at 743.081 kb on - strand, within RR42_RS03340 at 743.081 kb on - strand, within RR42_RS03340 at 743.114 kb on - strand, within RR42_RS03340 at 743.115 kb on + strand, within RR42_RS03340 at 743.115 kb on + strand, within RR42_RS03340 at 743.115 kb on + strand, within RR42_RS03340 at 743.116 kb on - strand, within RR42_RS03340 at 743.116 kb on - strand, within RR42_RS03340 at 743.209 kb on + strand, within RR42_RS03340 at 743.210 kb on - strand, within RR42_RS03340 at 743.210 kb on - strand, within RR42_RS03340 at 743.281 kb on + strand, within RR42_RS03340 at 743.418 kb on + strand, within RR42_RS03340 at 743.418 kb on + strand, within RR42_RS03340 at 743.424 kb on + strand, within RR42_RS03340 at 743.543 kb on + strand at 743.544 kb on - strand at 743.604 kb on - strand at 743.861 kb on + strand, within RR42_RS03345 at 743.862 kb on - strand, within RR42_RS03345 at 743.862 kb on - strand, within RR42_RS03345 at 744.011 kb on + strand, within RR42_RS03345 at 744.011 kb on + strand, within RR42_RS03345 at 744.011 kb on + strand, within RR42_RS03345 at 744.011 kb on + strand, within RR42_RS03345 at 744.011 kb on + strand, within RR42_RS03345 at 744.011 kb on + strand, within RR42_RS03345 at 744.012 kb on - strand, within RR42_RS03345 at 744.790 kb on + strand, within RR42_RS03350 at 744.790 kb on + strand, within RR42_RS03350 at 744.790 kb on + strand, within RR42_RS03350 at 744.791 kb on - strand, within RR42_RS03350 at 744.901 kb on + strand, within RR42_RS03350 at 744.901 kb on + strand, within RR42_RS03350 at 744.902 kb on - strand, within RR42_RS03350 at 744.904 kb on + strand, within RR42_RS03350 at 744.905 kb on - strand, within RR42_RS03350 at 744.905 kb on - strand at 744.905 kb on - strand, within RR42_RS03350 at 744.980 kb on - strand, within RR42_RS03350 at 744.980 kb on - strand, within RR42_RS03350 at 745.099 kb on - strand at 745.232 kb on + strand at 745.244 kb on + strand at 745.293 kb on + strand at 745.293 kb on + strand at 745.293 kb on + strand at 745.294 kb on - strand at 745.364 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Dimethyl Sulfoxide 5 vol% remove 743,016 + -0.9 743,017 - -1.3 743,017 - -1.3 743,081 - RR42_RS03340 0.13 -0.6 743,081 - RR42_RS03340 0.13 -0.8 743,114 - RR42_RS03340 0.20 +0.1 743,115 + RR42_RS03340 0.20 -0.8 743,115 + RR42_RS03340 0.20 +0.4 743,115 + RR42_RS03340 0.20 -0.7 743,116 - RR42_RS03340 0.21 +0.5 743,116 - RR42_RS03340 0.21 -2.9 743,209 + RR42_RS03340 0.39 -1.4 743,210 - RR42_RS03340 0.40 -1.1 743,210 - RR42_RS03340 0.40 -0.5 743,281 + RR42_RS03340 0.54 -1.0 743,418 + RR42_RS03340 0.82 -0.1 743,418 + RR42_RS03340 0.82 +0.5 743,424 + RR42_RS03340 0.83 -0.1 743,543 + -0.7 743,544 - +0.7 743,604 - -0.4 743,861 + RR42_RS03345 0.35 -0.6 743,862 - RR42_RS03345 0.35 -1.3 743,862 - RR42_RS03345 0.35 +0.0 744,011 + RR42_RS03345 0.51 -2.2 744,011 + RR42_RS03345 0.51 -0.4 744,011 + RR42_RS03345 0.51 -1.5 744,011 + RR42_RS03345 0.51 -1.0 744,011 + RR42_RS03345 0.51 -1.7 744,011 + RR42_RS03345 0.51 -2.0 744,012 - RR42_RS03345 0.51 -0.7 744,790 + RR42_RS03350 0.42 -0.3 744,790 + RR42_RS03350 0.42 -0.1 744,790 + RR42_RS03350 0.42 +1.3 744,791 - RR42_RS03350 0.42 +0.2 744,901 + RR42_RS03350 0.65 +0.9 744,901 + RR42_RS03350 0.65 -0.5 744,902 - RR42_RS03350 0.65 -0.3 744,904 + RR42_RS03350 0.66 -1.0 744,905 - RR42_RS03350 0.66 -1.6 744,905 - -0.3 744,905 - RR42_RS03350 0.66 -0.7 744,980 - RR42_RS03350 0.82 +0.0 744,980 - RR42_RS03350 0.82 +1.1 745,099 - +0.1 745,232 + -0.3 745,244 + +0.0 745,293 + -0.0 745,293 + -0.5 745,293 + +1.3 745,294 - -0.7 745,364 + -1.1
Or see this region's nucleotide sequence