Experiment: R2A with Dimethyl Sulfoxide 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS02890 and RR42_RS02895 are separated by 327 nucleotides RR42_RS02895 and RR42_RS02900 are separated by 117 nucleotides RR42_RS02900 and RR42_RS02905 are separated by 220 nucleotides
RR42_RS02890: RR42_RS02890 - 4-carboxymuconolactone decarboxylase, at 645,674 to 646,093
_RS02890
RR42_RS02895: RR42_RS02895 - hypothetical protein, at 646,421 to 646,651
_RS02895
RR42_RS02900: RR42_RS02900 - LysR family transcriptional regulator, at 646,769 to 647,710
_RS02900
RR42_RS02905: RR42_RS02905 - MFS transporter, at 647,931 to 648,929
_RS02905
Position (kb) 646
647
648 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 646.016 kb on + strand, within RR42_RS02890 at 646.016 kb on + strand, within RR42_RS02890 at 646.016 kb on + strand, within RR42_RS02890 at 646.016 kb on + strand, within RR42_RS02890 at 646.102 kb on + strand at 646.103 kb on - strand at 646.178 kb on + strand at 646.178 kb on + strand at 646.200 kb on - strand at 646.200 kb on - strand at 646.208 kb on + strand at 646.292 kb on - strand at 646.370 kb on - strand at 646.443 kb on + strand at 646.506 kb on + strand, within RR42_RS02895 at 646.506 kb on + strand, within RR42_RS02895 at 646.507 kb on - strand, within RR42_RS02895 at 646.507 kb on - strand, within RR42_RS02895 at 646.535 kb on + strand, within RR42_RS02895 at 646.536 kb on + strand, within RR42_RS02895 at 646.536 kb on + strand, within RR42_RS02895 at 646.536 kb on + strand, within RR42_RS02895 at 646.536 kb on + strand, within RR42_RS02895 at 646.536 kb on + strand, within RR42_RS02895 at 646.537 kb on - strand, within RR42_RS02895 at 646.537 kb on - strand, within RR42_RS02895 at 646.537 kb on - strand, within RR42_RS02895 at 646.537 kb on - strand, within RR42_RS02895 at 646.537 kb on - strand, within RR42_RS02895 at 646.537 kb on - strand, within RR42_RS02895 at 646.537 kb on - strand, within RR42_RS02895 at 646.656 kb on + strand at 646.657 kb on - strand at 646.657 kb on - strand at 646.741 kb on + strand at 646.770 kb on + strand at 646.902 kb on + strand, within RR42_RS02900 at 646.902 kb on + strand, within RR42_RS02900 at 646.902 kb on + strand, within RR42_RS02900 at 646.902 kb on + strand, within RR42_RS02900 at 646.902 kb on + strand, within RR42_RS02900 at 646.903 kb on - strand, within RR42_RS02900 at 646.903 kb on - strand, within RR42_RS02900 at 646.906 kb on - strand, within RR42_RS02900 at 646.965 kb on + strand, within RR42_RS02900 at 647.025 kb on - strand, within RR42_RS02900 at 647.046 kb on + strand, within RR42_RS02900 at 647.047 kb on - strand, within RR42_RS02900 at 647.250 kb on + strand, within RR42_RS02900 at 647.283 kb on + strand, within RR42_RS02900 at 647.283 kb on + strand, within RR42_RS02900 at 647.284 kb on - strand, within RR42_RS02900 at 647.489 kb on + strand, within RR42_RS02900 at 647.496 kb on + strand, within RR42_RS02900 at 647.496 kb on + strand, within RR42_RS02900 at 647.497 kb on - strand, within RR42_RS02900 at 647.502 kb on + strand, within RR42_RS02900 at 647.503 kb on - strand, within RR42_RS02900 at 647.791 kb on + strand at 647.791 kb on + strand at 647.791 kb on + strand at 647.791 kb on + strand at 648.024 kb on - strand at 648.201 kb on + strand, within RR42_RS02905 at 648.201 kb on + strand, within RR42_RS02905 at 648.252 kb on + strand, within RR42_RS02905 at 648.388 kb on - strand, within RR42_RS02905 at 648.504 kb on + strand, within RR42_RS02905 at 648.504 kb on + strand, within RR42_RS02905 at 648.504 kb on + strand, within RR42_RS02905 at 648.505 kb on - strand, within RR42_RS02905 at 648.566 kb on + strand, within RR42_RS02905 at 648.624 kb on - strand, within RR42_RS02905
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Dimethyl Sulfoxide 5 vol% remove 646,016 + RR42_RS02890 0.81 -0.7 646,016 + RR42_RS02890 0.81 +3.7 646,016 + RR42_RS02890 0.81 -0.4 646,016 + RR42_RS02890 0.81 -0.4 646,102 + -0.5 646,103 - -1.8 646,178 + +0.0 646,178 + -1.5 646,200 - -0.2 646,200 - +0.1 646,208 + -0.3 646,292 - +0.0 646,370 - +0.1 646,443 + +0.4 646,506 + RR42_RS02895 0.37 -1.3 646,506 + RR42_RS02895 0.37 -1.6 646,507 - RR42_RS02895 0.37 -0.4 646,507 - RR42_RS02895 0.37 -3.0 646,535 + RR42_RS02895 0.49 +0.9 646,536 + RR42_RS02895 0.50 -2.1 646,536 + RR42_RS02895 0.50 +0.4 646,536 + RR42_RS02895 0.50 -0.6 646,536 + RR42_RS02895 0.50 -1.0 646,536 + RR42_RS02895 0.50 -1.0 646,537 - RR42_RS02895 0.50 -0.4 646,537 - RR42_RS02895 0.50 -0.8 646,537 - RR42_RS02895 0.50 +0.8 646,537 - RR42_RS02895 0.50 -0.6 646,537 - RR42_RS02895 0.50 -2.0 646,537 - RR42_RS02895 0.50 -0.3 646,537 - RR42_RS02895 0.50 -0.9 646,656 + +0.8 646,657 - +0.4 646,657 - -0.4 646,741 + -0.8 646,770 + -0.2 646,902 + RR42_RS02900 0.14 +1.2 646,902 + RR42_RS02900 0.14 -1.5 646,902 + RR42_RS02900 0.14 -0.8 646,902 + RR42_RS02900 0.14 -0.8 646,902 + RR42_RS02900 0.14 -0.3 646,903 - RR42_RS02900 0.14 -2.0 646,903 - RR42_RS02900 0.14 +0.0 646,906 - RR42_RS02900 0.15 +0.5 646,965 + RR42_RS02900 0.21 +0.8 647,025 - RR42_RS02900 0.27 -0.4 647,046 + RR42_RS02900 0.29 -0.5 647,047 - RR42_RS02900 0.30 +0.1 647,250 + RR42_RS02900 0.51 +0.5 647,283 + RR42_RS02900 0.55 -0.0 647,283 + RR42_RS02900 0.55 -0.5 647,284 - RR42_RS02900 0.55 +0.4 647,489 + RR42_RS02900 0.76 -0.0 647,496 + RR42_RS02900 0.77 -0.8 647,496 + RR42_RS02900 0.77 -0.1 647,497 - RR42_RS02900 0.77 -0.6 647,502 + RR42_RS02900 0.78 +0.1 647,503 - RR42_RS02900 0.78 -0.3 647,791 + -1.6 647,791 + -0.4 647,791 + +0.0 647,791 + -0.2 648,024 - +0.0 648,201 + RR42_RS02905 0.27 +0.8 648,201 + RR42_RS02905 0.27 +0.6 648,252 + RR42_RS02905 0.32 +1.0 648,388 - RR42_RS02905 0.46 -1.0 648,504 + RR42_RS02905 0.57 +0.0 648,504 + RR42_RS02905 0.57 -2.3 648,504 + RR42_RS02905 0.57 -1.0 648,505 - RR42_RS02905 0.57 +0.0 648,566 + RR42_RS02905 0.64 +0.6 648,624 - RR42_RS02905 0.69 +0.0
Or see this region's nucleotide sequence