Experiment: R2A with Dimethyl Sulfoxide 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS02885 and RR42_RS02890 are separated by 60 nucleotides RR42_RS02890 and RR42_RS02895 are separated by 327 nucleotides RR42_RS02895 and RR42_RS02900 are separated by 117 nucleotides
RR42_RS02885: RR42_RS02885 - acetylornithine deacetylase, at 644,051 to 645,613
_RS02885
RR42_RS02890: RR42_RS02890 - 4-carboxymuconolactone decarboxylase, at 645,674 to 646,093
_RS02890
RR42_RS02895: RR42_RS02895 - hypothetical protein, at 646,421 to 646,651
_RS02895
RR42_RS02900: RR42_RS02900 - LysR family transcriptional regulator, at 646,769 to 647,710
_RS02900
Position (kb) 645
646
647 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 644.855 kb on + strand, within RR42_RS02885 at 644.856 kb on - strand, within RR42_RS02885 at 644.864 kb on + strand, within RR42_RS02885 at 644.864 kb on + strand, within RR42_RS02885 at 644.864 kb on + strand, within RR42_RS02885 at 644.865 kb on - strand, within RR42_RS02885 at 644.865 kb on - strand, within RR42_RS02885 at 644.865 kb on - strand, within RR42_RS02885 at 644.945 kb on + strand, within RR42_RS02885 at 644.948 kb on + strand, within RR42_RS02885 at 644.949 kb on - strand, within RR42_RS02885 at 644.949 kb on - strand, within RR42_RS02885 at 645.066 kb on - strand, within RR42_RS02885 at 645.294 kb on - strand, within RR42_RS02885 at 645.408 kb on - strand, within RR42_RS02885 at 645.533 kb on + strand at 645.534 kb on - strand at 645.534 kb on - strand at 645.560 kb on + strand at 645.561 kb on - strand at 645.561 kb on - strand at 645.611 kb on + strand at 645.611 kb on + strand at 645.667 kb on + strand at 645.668 kb on - strand at 645.668 kb on - strand at 645.741 kb on + strand, within RR42_RS02890 at 645.767 kb on + strand, within RR42_RS02890 at 645.767 kb on + strand, within RR42_RS02890 at 645.767 kb on + strand, within RR42_RS02890 at 645.768 kb on - strand, within RR42_RS02890 at 646.016 kb on + strand, within RR42_RS02890 at 646.016 kb on + strand, within RR42_RS02890 at 646.016 kb on + strand, within RR42_RS02890 at 646.016 kb on + strand, within RR42_RS02890 at 646.102 kb on + strand at 646.103 kb on - strand at 646.178 kb on + strand at 646.178 kb on + strand at 646.200 kb on - strand at 646.200 kb on - strand at 646.208 kb on + strand at 646.292 kb on - strand at 646.370 kb on - strand at 646.443 kb on + strand at 646.506 kb on + strand, within RR42_RS02895 at 646.506 kb on + strand, within RR42_RS02895 at 646.507 kb on - strand, within RR42_RS02895 at 646.507 kb on - strand, within RR42_RS02895 at 646.535 kb on + strand, within RR42_RS02895 at 646.536 kb on + strand, within RR42_RS02895 at 646.536 kb on + strand, within RR42_RS02895 at 646.536 kb on + strand, within RR42_RS02895 at 646.536 kb on + strand, within RR42_RS02895 at 646.536 kb on + strand, within RR42_RS02895 at 646.537 kb on - strand, within RR42_RS02895 at 646.537 kb on - strand, within RR42_RS02895 at 646.537 kb on - strand, within RR42_RS02895 at 646.537 kb on - strand, within RR42_RS02895 at 646.537 kb on - strand, within RR42_RS02895 at 646.537 kb on - strand, within RR42_RS02895 at 646.537 kb on - strand, within RR42_RS02895 at 646.656 kb on + strand at 646.657 kb on - strand at 646.657 kb on - strand at 646.741 kb on + strand at 646.770 kb on + strand at 646.902 kb on + strand, within RR42_RS02900 at 646.902 kb on + strand, within RR42_RS02900 at 646.902 kb on + strand, within RR42_RS02900 at 646.902 kb on + strand, within RR42_RS02900 at 646.902 kb on + strand, within RR42_RS02900 at 646.903 kb on - strand, within RR42_RS02900 at 646.903 kb on - strand, within RR42_RS02900 at 646.906 kb on - strand, within RR42_RS02900 at 646.965 kb on + strand, within RR42_RS02900 at 647.025 kb on - strand, within RR42_RS02900 at 647.046 kb on + strand, within RR42_RS02900 at 647.047 kb on - strand, within RR42_RS02900
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Dimethyl Sulfoxide 5 vol% remove 644,855 + RR42_RS02885 0.51 -0.3 644,856 - RR42_RS02885 0.52 -0.6 644,864 + RR42_RS02885 0.52 -0.3 644,864 + RR42_RS02885 0.52 -1.2 644,864 + RR42_RS02885 0.52 -0.2 644,865 - RR42_RS02885 0.52 -1.3 644,865 - RR42_RS02885 0.52 -0.5 644,865 - RR42_RS02885 0.52 -2.5 644,945 + RR42_RS02885 0.57 -1.5 644,948 + RR42_RS02885 0.57 +1.4 644,949 - RR42_RS02885 0.57 -0.2 644,949 - RR42_RS02885 0.57 -1.7 645,066 - RR42_RS02885 0.65 -0.6 645,294 - RR42_RS02885 0.80 -1.6 645,408 - RR42_RS02885 0.87 -0.1 645,533 + +0.8 645,534 - -1.8 645,534 - -0.1 645,560 + -0.7 645,561 - -1.2 645,561 - -1.2 645,611 + -0.8 645,611 + -1.3 645,667 + +0.1 645,668 - -1.6 645,668 - -0.7 645,741 + RR42_RS02890 0.16 -1.3 645,767 + RR42_RS02890 0.22 +0.5 645,767 + RR42_RS02890 0.22 +0.6 645,767 + RR42_RS02890 0.22 +0.8 645,768 - RR42_RS02890 0.22 -1.2 646,016 + RR42_RS02890 0.81 -0.7 646,016 + RR42_RS02890 0.81 +3.7 646,016 + RR42_RS02890 0.81 -0.4 646,016 + RR42_RS02890 0.81 -0.4 646,102 + -0.5 646,103 - -1.8 646,178 + +0.0 646,178 + -1.5 646,200 - -0.2 646,200 - +0.1 646,208 + -0.3 646,292 - +0.0 646,370 - +0.1 646,443 + +0.4 646,506 + RR42_RS02895 0.37 -1.3 646,506 + RR42_RS02895 0.37 -1.6 646,507 - RR42_RS02895 0.37 -0.4 646,507 - RR42_RS02895 0.37 -3.0 646,535 + RR42_RS02895 0.49 +0.9 646,536 + RR42_RS02895 0.50 -2.1 646,536 + RR42_RS02895 0.50 +0.4 646,536 + RR42_RS02895 0.50 -0.6 646,536 + RR42_RS02895 0.50 -1.0 646,536 + RR42_RS02895 0.50 -1.0 646,537 - RR42_RS02895 0.50 -0.4 646,537 - RR42_RS02895 0.50 -0.8 646,537 - RR42_RS02895 0.50 +0.8 646,537 - RR42_RS02895 0.50 -0.6 646,537 - RR42_RS02895 0.50 -2.0 646,537 - RR42_RS02895 0.50 -0.3 646,537 - RR42_RS02895 0.50 -0.9 646,656 + +0.8 646,657 - +0.4 646,657 - -0.4 646,741 + -0.8 646,770 + -0.2 646,902 + RR42_RS02900 0.14 +1.2 646,902 + RR42_RS02900 0.14 -1.5 646,902 + RR42_RS02900 0.14 -0.8 646,902 + RR42_RS02900 0.14 -0.8 646,902 + RR42_RS02900 0.14 -0.3 646,903 - RR42_RS02900 0.14 -2.0 646,903 - RR42_RS02900 0.14 +0.0 646,906 - RR42_RS02900 0.15 +0.5 646,965 + RR42_RS02900 0.21 +0.8 647,025 - RR42_RS02900 0.27 -0.4 647,046 + RR42_RS02900 0.29 -0.5 647,047 - RR42_RS02900 0.30 +0.1
Or see this region's nucleotide sequence