Experiment: R2A with Dimethyl Sulfoxide 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS02315 and RR42_RS02320 are separated by 243 nucleotides RR42_RS02320 and RR42_RS02325 are separated by 12 nucleotides
RR42_RS02315: RR42_RS02315 - threonine dehydratase, at 518,349 to 519,875
_RS02315
RR42_RS02320: RR42_RS02320 - trehalose phosphatase, at 520,119 to 520,904
_RS02320
RR42_RS02325: RR42_RS02325 - glucoamylase, at 520,917 to 522,755
_RS02325
Position (kb) 520
521 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 519.264 kb on + strand, within RR42_RS02315 at 519.265 kb on - strand, within RR42_RS02315 at 519.265 kb on - strand, within RR42_RS02315 at 519.296 kb on - strand, within RR42_RS02315 at 519.478 kb on + strand, within RR42_RS02315 at 519.479 kb on - strand, within RR42_RS02315 at 519.479 kb on - strand, within RR42_RS02315 at 519.479 kb on - strand, within RR42_RS02315 at 519.479 kb on - strand, within RR42_RS02315 at 519.491 kb on - strand, within RR42_RS02315 at 519.703 kb on + strand, within RR42_RS02315 at 519.797 kb on - strand at 519.797 kb on - strand at 519.826 kb on + strand at 519.826 kb on + strand at 519.856 kb on + strand at 519.856 kb on + strand at 519.857 kb on - strand at 519.918 kb on - strand at 519.918 kb on - strand at 520.066 kb on + strand at 520.112 kb on + strand at 520.113 kb on - strand at 520.130 kb on - strand at 520.497 kb on + strand, within RR42_RS02320 at 520.646 kb on + strand, within RR42_RS02320 at 520.697 kb on + strand, within RR42_RS02320 at 520.697 kb on + strand, within RR42_RS02320 at 520.698 kb on - strand, within RR42_RS02320 at 521.169 kb on + strand, within RR42_RS02325 at 521.169 kb on + strand, within RR42_RS02325 at 521.170 kb on - strand, within RR42_RS02325 at 521.235 kb on - strand, within RR42_RS02325 at 521.271 kb on + strand, within RR42_RS02325 at 521.272 kb on - strand, within RR42_RS02325 at 521.406 kb on + strand, within RR42_RS02325 at 521.407 kb on - strand, within RR42_RS02325 at 521.408 kb on + strand, within RR42_RS02325 at 521.457 kb on + strand, within RR42_RS02325 at 521.457 kb on + strand, within RR42_RS02325 at 521.457 kb on + strand, within RR42_RS02325 at 521.457 kb on + strand, within RR42_RS02325 at 521.458 kb on - strand, within RR42_RS02325 at 521.580 kb on + strand, within RR42_RS02325 at 521.580 kb on + strand, within RR42_RS02325 at 521.580 kb on + strand, within RR42_RS02325 at 521.581 kb on - strand, within RR42_RS02325 at 521.651 kb on + strand, within RR42_RS02325 at 521.651 kb on + strand, within RR42_RS02325 at 521.671 kb on - strand, within RR42_RS02325 at 521.671 kb on - strand, within RR42_RS02325 at 521.829 kb on + strand, within RR42_RS02325 at 521.889 kb on + strand, within RR42_RS02325
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Dimethyl Sulfoxide 5 vol% remove 519,264 + RR42_RS02315 0.60 -0.2 519,265 - RR42_RS02315 0.60 -1.1 519,265 - RR42_RS02315 0.60 -0.6 519,296 - RR42_RS02315 0.62 +3.0 519,478 + RR42_RS02315 0.74 -0.8 519,479 - RR42_RS02315 0.74 -0.2 519,479 - RR42_RS02315 0.74 -0.5 519,479 - RR42_RS02315 0.74 +0.1 519,479 - RR42_RS02315 0.74 -0.2 519,491 - RR42_RS02315 0.75 +0.1 519,703 + RR42_RS02315 0.89 -1.1 519,797 - -0.5 519,797 - -0.0 519,826 + +0.7 519,826 + -0.5 519,856 + -0.2 519,856 + -0.8 519,857 - -0.3 519,918 - -0.0 519,918 - +0.5 520,066 + +0.1 520,112 + -1.0 520,113 - -1.0 520,130 - +0.4 520,497 + RR42_RS02320 0.48 -0.2 520,646 + RR42_RS02320 0.67 +0.3 520,697 + RR42_RS02320 0.74 -1.9 520,697 + RR42_RS02320 0.74 -0.0 520,698 - RR42_RS02320 0.74 -1.5 521,169 + RR42_RS02325 0.14 -1.2 521,169 + RR42_RS02325 0.14 -1.1 521,170 - RR42_RS02325 0.14 +0.2 521,235 - RR42_RS02325 0.17 -0.6 521,271 + RR42_RS02325 0.19 +0.7 521,272 - RR42_RS02325 0.19 -0.5 521,406 + RR42_RS02325 0.27 +0.8 521,407 - RR42_RS02325 0.27 -0.5 521,408 + RR42_RS02325 0.27 +0.1 521,457 + RR42_RS02325 0.29 -2.3 521,457 + RR42_RS02325 0.29 +0.7 521,457 + RR42_RS02325 0.29 -0.6 521,457 + RR42_RS02325 0.29 -1.6 521,458 - RR42_RS02325 0.29 -0.7 521,580 + RR42_RS02325 0.36 +0.1 521,580 + RR42_RS02325 0.36 -0.7 521,580 + RR42_RS02325 0.36 -1.7 521,581 - RR42_RS02325 0.36 -0.0 521,651 + RR42_RS02325 0.40 -1.6 521,651 + RR42_RS02325 0.40 -0.5 521,671 - RR42_RS02325 0.41 +0.6 521,671 - RR42_RS02325 0.41 +1.2 521,829 + RR42_RS02325 0.50 -1.6 521,889 + RR42_RS02325 0.53 +1.1
Or see this region's nucleotide sequence