Experiment: R2A with Dimethyl Sulfoxide 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS02225 and RR42_RS02230 are separated by 67 nucleotides RR42_RS02230 and RR42_RS02235 overlap by 4 nucleotides RR42_RS02235 and RR42_RS02240 overlap by 1 nucleotides
RR42_RS02225: RR42_RS02225 - ABC transporter substrate-binding protein, at 492,781 to 493,764
_RS02225
RR42_RS02230: RR42_RS02230 - sulfolactate dehydrogenase, at 493,832 to 494,836
_RS02230
RR42_RS02235: RR42_RS02235 - 3-phosphoglycerate dehydrogenase, at 494,833 to 495,768
_RS02235
RR42_RS02240: RR42_RS02240 - D-galactarate dehydratase, at 495,768 to 496,943
_RS02240
Position (kb) 493
494
495 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 492.845 kb on + strand at 493.416 kb on + strand, within RR42_RS02225 at 493.524 kb on + strand, within RR42_RS02225 at 493.525 kb on - strand, within RR42_RS02225 at 493.646 kb on + strand, within RR42_RS02225 at 493.646 kb on + strand, within RR42_RS02225 at 493.739 kb on - strand at 493.845 kb on - strand at 493.992 kb on + strand, within RR42_RS02230 at 493.993 kb on - strand, within RR42_RS02230 at 493.993 kb on - strand, within RR42_RS02230 at 494.048 kb on - strand, within RR42_RS02230 at 494.627 kb on + strand, within RR42_RS02230 at 494.679 kb on + strand, within RR42_RS02230 at 494.679 kb on + strand, within RR42_RS02230 at 494.680 kb on - strand, within RR42_RS02230 at 494.920 kb on - strand at 494.959 kb on - strand, within RR42_RS02235 at 494.971 kb on + strand, within RR42_RS02235 at 495.416 kb on + strand, within RR42_RS02235 at 495.455 kb on + strand, within RR42_RS02235 at 495.535 kb on - strand, within RR42_RS02235
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Dimethyl Sulfoxide 5 vol% remove 492,845 + +0.2 493,416 + RR42_RS02225 0.65 -2.2 493,524 + RR42_RS02225 0.76 -1.8 493,525 - RR42_RS02225 0.76 +1.4 493,646 + RR42_RS02225 0.88 -2.5 493,646 + RR42_RS02225 0.88 -0.5 493,739 - +1.2 493,845 - -0.7 493,992 + RR42_RS02230 0.16 -0.3 493,993 - RR42_RS02230 0.16 +0.5 493,993 - RR42_RS02230 0.16 +0.4 494,048 - RR42_RS02230 0.21 +0.7 494,627 + RR42_RS02230 0.79 +0.4 494,679 + RR42_RS02230 0.84 +0.6 494,679 + RR42_RS02230 0.84 +0.3 494,680 - RR42_RS02230 0.84 +2.1 494,920 - -0.9 494,959 - RR42_RS02235 0.13 -0.6 494,971 + RR42_RS02235 0.15 -0.1 495,416 + RR42_RS02235 0.62 -0.8 495,455 + RR42_RS02235 0.66 -2.8 495,535 - RR42_RS02235 0.75 +0.1
Or see this region's nucleotide sequence