Experiment: R2A with Dimethyl Sulfoxide 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS01820 and RR42_RS01825 are separated by 161 nucleotides RR42_RS01825 and RR42_RS01830 are separated by 140 nucleotides RR42_RS01830 and RR42_RS01835 are separated by 118 nucleotides
RR42_RS01820: RR42_RS01820 - peptidase S41, at 413,123 to 414,712
_RS01820
RR42_RS01825: RR42_RS01825 - phosphoglyceromutase, at 414,874 to 415,620
_RS01825
RR42_RS01830: RR42_RS01830 - sulfurtransferase, at 415,761 to 416,174
_RS01830
RR42_RS01835: RR42_RS01835 - glutaredoxin, at 416,293 to 416,550
_RS01835
Position (kb) 414
415
416 Strain fitness (log2 ratio) -2
-1
0
1
2 at 413.887 kb on - strand, within RR42_RS01820 at 414.211 kb on - strand, within RR42_RS01820 at 414.211 kb on - strand, within RR42_RS01820 at 414.211 kb on - strand, within RR42_RS01820 at 414.550 kb on - strand, within RR42_RS01820 at 414.550 kb on - strand, within RR42_RS01820 at 414.680 kb on - strand at 414.793 kb on - strand at 414.793 kb on - strand at 414.793 kb on - strand at 414.793 kb on - strand at 414.793 kb on - strand at 414.794 kb on + strand at 414.794 kb on + strand at 414.794 kb on + strand at 414.794 kb on + strand at 414.794 kb on + strand at 414.794 kb on + strand at 414.794 kb on + strand at 414.794 kb on + strand at 414.795 kb on - strand at 414.795 kb on - strand at 414.795 kb on - strand at 414.795 kb on - strand at 414.795 kb on - strand at 414.795 kb on - strand at 414.795 kb on - strand at 414.795 kb on - strand at 415.664 kb on - strand at 415.685 kb on - strand at 415.725 kb on + strand at 415.725 kb on + strand at 415.726 kb on - strand at 415.726 kb on - strand at 415.727 kb on + strand at 415.727 kb on + strand at 415.728 kb on - strand at 415.780 kb on - strand at 415.780 kb on - strand at 415.944 kb on + strand, within RR42_RS01830 at 415.944 kb on + strand, within RR42_RS01830 at 415.945 kb on - strand, within RR42_RS01830 at 416.103 kb on + strand, within RR42_RS01830 at 416.104 kb on - strand, within RR42_RS01830 at 416.116 kb on - strand, within RR42_RS01830 at 416.116 kb on - strand, within RR42_RS01830 at 416.172 kb on + strand at 416.173 kb on - strand at 416.173 kb on - strand at 416.306 kb on + strand at 416.306 kb on + strand at 416.306 kb on + strand at 416.306 kb on + strand at 416.306 kb on + strand at 416.306 kb on + strand at 416.307 kb on - strand at 416.311 kb on + strand at 416.312 kb on - strand at 416.321 kb on + strand, within RR42_RS01835 at 416.321 kb on + strand, within RR42_RS01835 at 416.322 kb on - strand, within RR42_RS01835 at 416.322 kb on - strand, within RR42_RS01835 at 416.464 kb on + strand, within RR42_RS01835 at 416.464 kb on + strand, within RR42_RS01835 at 416.596 kb on + strand at 416.596 kb on + strand at 416.596 kb on + strand at 416.597 kb on - strand at 416.597 kb on - strand at 416.597 kb on - strand at 416.597 kb on - strand at 416.601 kb on + strand at 416.601 kb on + strand at 416.601 kb on + strand at 416.601 kb on + strand at 416.601 kb on + strand at 416.601 kb on + strand at 416.601 kb on + strand at 416.603 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Dimethyl Sulfoxide 5 vol% remove 413,887 - RR42_RS01820 0.48 +0.4 414,211 - RR42_RS01820 0.68 -2.4 414,211 - RR42_RS01820 0.68 -1.8 414,211 - RR42_RS01820 0.68 -2.1 414,550 - RR42_RS01820 0.90 -2.5 414,550 - RR42_RS01820 0.90 -2.7 414,680 - -0.6 414,793 - +0.3 414,793 - -0.2 414,793 - -1.3 414,793 - +0.2 414,793 - +1.2 414,794 + -0.3 414,794 + -0.8 414,794 + -0.6 414,794 + -0.2 414,794 + -0.8 414,794 + -2.1 414,794 + +0.7 414,794 + -0.5 414,795 - +0.6 414,795 - +0.2 414,795 - +0.3 414,795 - -1.9 414,795 - -0.2 414,795 - -0.6 414,795 - -0.3 414,795 - -0.4 415,664 - +0.1 415,685 - +1.2 415,725 + -1.9 415,725 + -0.3 415,726 - +1.9 415,726 - -0.0 415,727 + -0.2 415,727 + +0.3 415,728 - -0.4 415,780 - -1.6 415,780 - -0.2 415,944 + RR42_RS01830 0.44 -0.8 415,944 + RR42_RS01830 0.44 -0.3 415,945 - RR42_RS01830 0.44 -1.4 416,103 + RR42_RS01830 0.83 -0.3 416,104 - RR42_RS01830 0.83 -0.4 416,116 - RR42_RS01830 0.86 -0.9 416,116 - RR42_RS01830 0.86 +0.4 416,172 + -0.7 416,173 - +0.2 416,173 - -2.0 416,306 + -1.2 416,306 + -0.2 416,306 + -2.0 416,306 + +1.0 416,306 + +0.2 416,306 + -0.0 416,307 - +0.3 416,311 + +0.2 416,312 - -0.1 416,321 + RR42_RS01835 0.11 +0.0 416,321 + RR42_RS01835 0.11 -0.2 416,322 - RR42_RS01835 0.11 -1.3 416,322 - RR42_RS01835 0.11 -1.6 416,464 + RR42_RS01835 0.66 -0.3 416,464 + RR42_RS01835 0.66 +0.4 416,596 + -0.1 416,596 + -0.2 416,596 + -0.5 416,597 - +0.7 416,597 - -0.0 416,597 - +0.5 416,597 - +0.1 416,601 + +0.3 416,601 + -1.0 416,601 + -0.5 416,601 + +0.4 416,601 + -2.1 416,601 + -0.3 416,601 + -1.0 416,603 + +0.4
Or see this region's nucleotide sequence