Experiment: R2A with Dimethyl Sulfoxide 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS01125 and RR42_RS01130 are separated by 55 nucleotides RR42_RS01130 and RR42_RS01135 overlap by 4 nucleotides RR42_RS01135 and RR42_RS01140 are separated by 50 nucleotides RR42_RS01140 and RR42_RS01145 are separated by 16 nucleotides
RR42_RS01125: RR42_RS01125 - acetylglutamate kinase, at 259,367 to 260,284
_RS01125
RR42_RS01130: RR42_RS01130 - haloacid dehalogenase, at 260,340 to 261,320
_RS01130
RR42_RS01135: RR42_RS01135 - dihydroorotate oxidase, at 261,317 to 261,976
_RS01135
RR42_RS01140: RR42_RS01140 - hypothetical protein, at 262,027 to 262,260
_RS01140
RR42_RS01145: RR42_RS01145 - membrane protein, at 262,277 to 263,710
_RS01145
Position (kb) 260
261
262 Strain fitness (log2 ratio) -2
-1
0
1
2 at 260.282 kb on + strand at 260.293 kb on + strand at 260.294 kb on - strand at 260.294 kb on - strand at 260.349 kb on - strand at 260.349 kb on - strand at 260.349 kb on - strand at 260.505 kb on + strand, within RR42_RS01130 at 260.505 kb on + strand, within RR42_RS01130 at 260.506 kb on - strand, within RR42_RS01130 at 260.614 kb on + strand, within RR42_RS01130 at 260.615 kb on - strand, within RR42_RS01130 at 260.751 kb on + strand, within RR42_RS01130 at 260.986 kb on - strand, within RR42_RS01130 at 260.986 kb on - strand, within RR42_RS01130 at 261.265 kb on + strand at 261.265 kb on + strand at 261.265 kb on + strand at 261.272 kb on - strand at 261.974 kb on + strand at 261.975 kb on - strand at 262.028 kb on + strand at 262.250 kb on + strand at 262.250 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Dimethyl Sulfoxide 5 vol% remove 260,282 + +0.3 260,293 + -0.5 260,294 - +0.3 260,294 - +0.8 260,349 - +0.2 260,349 - +0.4 260,349 - +0.8 260,505 + RR42_RS01130 0.17 +0.1 260,505 + RR42_RS01130 0.17 +0.4 260,506 - RR42_RS01130 0.17 -0.2 260,614 + RR42_RS01130 0.28 +1.5 260,615 - RR42_RS01130 0.28 +1.9 260,751 + RR42_RS01130 0.42 +2.5 260,986 - RR42_RS01130 0.66 -0.2 260,986 - RR42_RS01130 0.66 -1.0 261,265 + +0.0 261,265 + +1.0 261,265 + -1.0 261,272 - +0.4 261,974 + -0.2 261,975 - +1.5 262,028 + -0.4 262,250 + -0.6 262,250 + -2.2
Or see this region's nucleotide sequence