Experiment: R2A with Dimethyl Sulfoxide 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS00745 and RR42_RS00750 are separated by 58 nucleotides RR42_RS00750 and RR42_RS00755 are separated by 123 nucleotides
RR42_RS00745: RR42_RS00745 - IclR family transcriptional regulator, at 187,547 to 188,383
_RS00745
RR42_RS00750: RR42_RS00750 - hypothetical protein, at 188,442 to 189,422
_RS00750
RR42_RS00755: RR42_RS00755 - 3-oxoadipate enol-lactonase, at 189,546 to 190,388
_RS00755
Position (kb) 188
189
190 Strain fitness (log2 ratio) -1
0
1
2 at 187.444 kb on + strand at 187.445 kb on - strand at 187.445 kb on - strand at 187.446 kb on + strand at 187.450 kb on - strand at 187.450 kb on - strand at 187.498 kb on - strand at 187.498 kb on - strand at 187.507 kb on + strand at 187.507 kb on + strand at 187.508 kb on - strand at 187.508 kb on - strand at 187.706 kb on + strand, within RR42_RS00745 at 187.764 kb on - strand, within RR42_RS00745 at 188.015 kb on + strand, within RR42_RS00745 at 188.016 kb on - strand, within RR42_RS00745 at 188.016 kb on - strand, within RR42_RS00745 at 188.081 kb on - strand, within RR42_RS00745 at 188.081 kb on - strand, within RR42_RS00745 at 188.081 kb on - strand, within RR42_RS00745 at 188.081 kb on - strand, within RR42_RS00745 at 188.111 kb on + strand at 188.111 kb on + strand, within RR42_RS00745 at 188.112 kb on - strand, within RR42_RS00745 at 188.112 kb on - strand, within RR42_RS00745 at 188.259 kb on + strand, within RR42_RS00745 at 188.259 kb on + strand, within RR42_RS00745 at 188.260 kb on - strand, within RR42_RS00745 at 188.336 kb on + strand at 188.336 kb on + strand at 188.468 kb on + strand at 188.468 kb on + strand at 188.469 kb on - strand at 188.469 kb on - strand at 188.883 kb on + strand, within RR42_RS00750 at 189.145 kb on - strand, within RR42_RS00750 at 189.145 kb on - strand, within RR42_RS00750 at 189.216 kb on + strand, within RR42_RS00750 at 189.216 kb on + strand, within RR42_RS00750 at 189.216 kb on + strand, within RR42_RS00750 at 189.216 kb on + strand, within RR42_RS00750 at 189.216 kb on + strand, within RR42_RS00750 at 189.216 kb on + strand, within RR42_RS00750 at 189.216 kb on + strand, within RR42_RS00750 at 189.216 kb on + strand, within RR42_RS00750 at 189.216 kb on + strand, within RR42_RS00750 at 189.217 kb on - strand, within RR42_RS00750 at 189.217 kb on - strand, within RR42_RS00750 at 189.217 kb on - strand, within RR42_RS00750 at 189.239 kb on + strand, within RR42_RS00750 at 189.450 kb on - strand at 189.727 kb on - strand, within RR42_RS00755 at 189.772 kb on - strand, within RR42_RS00755 at 189.772 kb on - strand, within RR42_RS00755 at 190.184 kb on + strand, within RR42_RS00755 at 190.184 kb on + strand, within RR42_RS00755 at 190.305 kb on - strand at 190.381 kb on + strand at 190.381 kb on + strand at 190.381 kb on + strand at 190.381 kb on + strand at 190.382 kb on - strand at 190.382 kb on - strand at 190.382 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Dimethyl Sulfoxide 5 vol% remove 187,444 + -0.8 187,445 - +1.0 187,445 - -0.1 187,446 + -1.1 187,450 - -0.4 187,450 - +0.5 187,498 - -0.2 187,498 - -0.9 187,507 + -1.4 187,507 + +1.0 187,508 - -0.7 187,508 - -1.0 187,706 + RR42_RS00745 0.19 -0.4 187,764 - RR42_RS00745 0.26 -1.1 188,015 + RR42_RS00745 0.56 -0.4 188,016 - RR42_RS00745 0.56 -0.5 188,016 - RR42_RS00745 0.56 -1.3 188,081 - RR42_RS00745 0.64 -0.1 188,081 - RR42_RS00745 0.64 -1.0 188,081 - RR42_RS00745 0.64 +0.2 188,081 - RR42_RS00745 0.64 -0.3 188,111 + +1.5 188,111 + RR42_RS00745 0.67 -0.0 188,112 - RR42_RS00745 0.68 +0.9 188,112 - RR42_RS00745 0.68 -0.8 188,259 + RR42_RS00745 0.85 -1.2 188,259 + RR42_RS00745 0.85 -0.1 188,260 - RR42_RS00745 0.85 -0.4 188,336 + +0.3 188,336 + -0.4 188,468 + -1.5 188,468 + +1.5 188,469 - +0.4 188,469 - -1.0 188,883 + RR42_RS00750 0.45 -0.1 189,145 - RR42_RS00750 0.72 -0.1 189,145 - RR42_RS00750 0.72 -0.8 189,216 + RR42_RS00750 0.79 -1.1 189,216 + RR42_RS00750 0.79 -0.6 189,216 + RR42_RS00750 0.79 -0.9 189,216 + RR42_RS00750 0.79 -0.7 189,216 + RR42_RS00750 0.79 -0.9 189,216 + RR42_RS00750 0.79 +0.2 189,216 + RR42_RS00750 0.79 -0.9 189,216 + RR42_RS00750 0.79 +0.2 189,216 + RR42_RS00750 0.79 -0.1 189,217 - RR42_RS00750 0.79 -0.2 189,217 - RR42_RS00750 0.79 -0.2 189,217 - RR42_RS00750 0.79 -1.6 189,239 + RR42_RS00750 0.81 +1.8 189,450 - +0.3 189,727 - RR42_RS00755 0.21 +0.2 189,772 - RR42_RS00755 0.27 -0.3 189,772 - RR42_RS00755 0.27 -0.4 190,184 + RR42_RS00755 0.76 +1.1 190,184 + RR42_RS00755 0.76 +0.5 190,305 - -1.6 190,381 + -0.1 190,381 + +0.1 190,381 + -0.3 190,381 + +0.4 190,382 - -0.0 190,382 - -0.8 190,382 - -1.6
Or see this region's nucleotide sequence