Experiment: R2A with Dimethyl Sulfoxide 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS00695 and RR42_RS00700 overlap by 4 nucleotides RR42_RS00700 and RR42_RS00705 are separated by 57 nucleotides RR42_RS00705 and RR42_RS00710 are separated by 28 nucleotides
RR42_RS00695: RR42_RS00695 - biotin--[acetyl-CoA-carboxylase] synthetase, at 176,821 to 177,705
_RS00695
RR42_RS00700: RR42_RS00700 - pantothenate kinase, at 177,702 to 178,544
_RS00700
RR42_RS00705: RR42_RS00705 - hypothetical protein, at 178,602 to 179,330
_RS00705
RR42_RS00710: RR42_RS00710 - ADP-heptose synthase, at 179,359 to 179,847
_RS00710
Position (kb) 178
179
180 Strain fitness (log2 ratio) -2
-1
0
1
2 at 178.567 kb on + strand at 178.567 kb on + strand at 178.568 kb on - strand at 178.621 kb on + strand at 179.069 kb on + strand, within RR42_RS00705 at 179.070 kb on - strand, within RR42_RS00705 at 179.080 kb on + strand, within RR42_RS00705 at 179.080 kb on + strand, within RR42_RS00705 at 179.080 kb on + strand, within RR42_RS00705 at 179.080 kb on + strand, within RR42_RS00705 at 179.081 kb on - strand, within RR42_RS00705 at 179.081 kb on - strand, within RR42_RS00705 at 179.081 kb on - strand, within RR42_RS00705 at 179.112 kb on + strand, within RR42_RS00705 at 179.112 kb on + strand, within RR42_RS00705 at 179.113 kb on - strand, within RR42_RS00705 at 179.113 kb on - strand, within RR42_RS00705 at 179.113 kb on - strand, within RR42_RS00705 at 179.113 kb on - strand, within RR42_RS00705 at 179.113 kb on - strand, within RR42_RS00705 at 179.114 kb on + strand, within RR42_RS00705 at 179.115 kb on - strand, within RR42_RS00705 at 179.115 kb on - strand, within RR42_RS00705 at 179.937 kb on + strand at 179.937 kb on + strand at 179.937 kb on + strand at 179.937 kb on + strand at 179.938 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Dimethyl Sulfoxide 5 vol% remove 178,567 + -0.8 178,567 + +0.3 178,568 - +1.4 178,621 + +0.0 179,069 + RR42_RS00705 0.64 -0.4 179,070 - RR42_RS00705 0.64 -0.2 179,080 + RR42_RS00705 0.66 -0.3 179,080 + RR42_RS00705 0.66 +2.2 179,080 + RR42_RS00705 0.66 -0.6 179,080 + RR42_RS00705 0.66 +0.1 179,081 - RR42_RS00705 0.66 -0.7 179,081 - RR42_RS00705 0.66 -0.0 179,081 - RR42_RS00705 0.66 +0.7 179,112 + RR42_RS00705 0.70 -0.5 179,112 + RR42_RS00705 0.70 -0.4 179,113 - RR42_RS00705 0.70 +0.0 179,113 - RR42_RS00705 0.70 -0.9 179,113 - RR42_RS00705 0.70 +1.5 179,113 - RR42_RS00705 0.70 -1.4 179,113 - RR42_RS00705 0.70 -0.2 179,114 + RR42_RS00705 0.70 +0.0 179,115 - RR42_RS00705 0.70 -0.4 179,115 - RR42_RS00705 0.70 +0.6 179,937 + -2.6 179,937 + -0.3 179,937 + -2.1 179,937 + -1.1 179,938 - -0.2
Or see this region's nucleotide sequence