Experiment: R2A with Dimethyl Sulfoxide 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS00605 and RR42_RS00610 are separated by 169 nucleotides RR42_RS00610 and RR42_RS00615 are separated by 35 nucleotides RR42_RS00615 and RR42_RS00620 overlap by 4 nucleotides
RR42_RS00605: RR42_RS00605 - glutamyl-tRNA amidotransferase, at 158,952 to 160,412
_RS00605
RR42_RS00610: RR42_RS00610 - hypothetical protein, at 160,582 to 161,250
_RS00610
RR42_RS00615: RR42_RS00615 - recombinase XerC, at 161,286 to 162,410
_RS00615
RR42_RS00620: RR42_RS00620 - XRE family transcriptional regulator, at 162,407 to 163,366
_RS00620
Position (kb) 161
162
163 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 160.410 kb on + strand at 160.420 kb on - strand at 160.457 kb on + strand at 160.458 kb on - strand at 160.550 kb on - strand at 160.606 kb on + strand at 160.606 kb on + strand at 160.607 kb on - strand at 160.861 kb on + strand at 160.862 kb on - strand, within RR42_RS00610 at 160.939 kb on + strand, within RR42_RS00610 at 160.939 kb on + strand, within RR42_RS00610 at 160.940 kb on - strand, within RR42_RS00610 at 160.940 kb on - strand, within RR42_RS00610 at 160.940 kb on - strand, within RR42_RS00610 at 160.940 kb on - strand, within RR42_RS00610 at 161.011 kb on + strand, within RR42_RS00610 at 161.195 kb on - strand at 161.422 kb on - strand, within RR42_RS00615 at 161.505 kb on - strand, within RR42_RS00615 at 161.772 kb on + strand, within RR42_RS00615 at 161.772 kb on + strand, within RR42_RS00615 at 161.820 kb on + strand, within RR42_RS00615 at 161.839 kb on - strand, within RR42_RS00615 at 162.051 kb on - strand, within RR42_RS00615 at 162.219 kb on + strand, within RR42_RS00615 at 162.219 kb on + strand, within RR42_RS00615 at 162.219 kb on + strand, within RR42_RS00615 at 162.219 kb on + strand, within RR42_RS00615 at 162.220 kb on - strand, within RR42_RS00615 at 162.220 kb on - strand, within RR42_RS00615 at 162.255 kb on + strand, within RR42_RS00615 at 162.255 kb on + strand, within RR42_RS00615 at 162.479 kb on + strand at 162.479 kb on + strand at 162.479 kb on + strand at 162.480 kb on - strand at 162.480 kb on - strand at 162.519 kb on + strand, within RR42_RS00620 at 162.519 kb on + strand, within RR42_RS00620 at 162.538 kb on + strand, within RR42_RS00620 at 162.538 kb on + strand, within RR42_RS00620 at 162.538 kb on + strand, within RR42_RS00620 at 162.538 kb on + strand, within RR42_RS00620 at 162.545 kb on + strand, within RR42_RS00620 at 162.545 kb on + strand, within RR42_RS00620 at 162.545 kb on + strand, within RR42_RS00620 at 162.545 kb on + strand, within RR42_RS00620 at 162.546 kb on - strand, within RR42_RS00620 at 162.546 kb on - strand, within RR42_RS00620 at 162.546 kb on - strand, within RR42_RS00620 at 162.546 kb on - strand, within RR42_RS00620 at 162.588 kb on + strand, within RR42_RS00620 at 162.588 kb on + strand, within RR42_RS00620 at 162.588 kb on + strand, within RR42_RS00620 at 162.849 kb on + strand, within RR42_RS00620 at 162.849 kb on + strand, within RR42_RS00620 at 162.849 kb on + strand, within RR42_RS00620 at 162.849 kb on + strand, within RR42_RS00620 at 162.850 kb on - strand, within RR42_RS00620 at 162.850 kb on - strand, within RR42_RS00620 at 162.850 kb on - strand, within RR42_RS00620 at 162.850 kb on - strand, within RR42_RS00620 at 162.850 kb on - strand, within RR42_RS00620
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Dimethyl Sulfoxide 5 vol% remove 160,410 + -1.4 160,420 - -1.8 160,457 + +0.1 160,458 - -1.7 160,550 - +0.7 160,606 + -1.1 160,606 + -0.8 160,607 - -0.2 160,861 + +1.5 160,862 - RR42_RS00610 0.42 -1.0 160,939 + RR42_RS00610 0.53 +0.1 160,939 + RR42_RS00610 0.53 -1.4 160,940 - RR42_RS00610 0.54 -1.2 160,940 - RR42_RS00610 0.54 -0.6 160,940 - RR42_RS00610 0.54 +0.4 160,940 - RR42_RS00610 0.54 -1.7 161,011 + RR42_RS00610 0.64 -0.8 161,195 - +0.1 161,422 - RR42_RS00615 0.12 -0.8 161,505 - RR42_RS00615 0.19 -0.3 161,772 + RR42_RS00615 0.43 -0.6 161,772 + RR42_RS00615 0.43 -0.7 161,820 + RR42_RS00615 0.47 -1.4 161,839 - RR42_RS00615 0.49 -3.4 162,051 - RR42_RS00615 0.68 -0.5 162,219 + RR42_RS00615 0.83 -0.6 162,219 + RR42_RS00615 0.83 -0.9 162,219 + RR42_RS00615 0.83 -0.1 162,219 + RR42_RS00615 0.83 -1.6 162,220 - RR42_RS00615 0.83 +0.0 162,220 - RR42_RS00615 0.83 -0.6 162,255 + RR42_RS00615 0.86 +1.1 162,255 + RR42_RS00615 0.86 -1.5 162,479 + -0.6 162,479 + +0.5 162,479 + -0.4 162,480 - +2.1 162,480 - -0.5 162,519 + RR42_RS00620 0.12 -0.5 162,519 + RR42_RS00620 0.12 -0.2 162,538 + RR42_RS00620 0.14 +0.3 162,538 + RR42_RS00620 0.14 -0.8 162,538 + RR42_RS00620 0.14 -0.1 162,538 + RR42_RS00620 0.14 -0.1 162,545 + RR42_RS00620 0.14 -1.8 162,545 + RR42_RS00620 0.14 -1.4 162,545 + RR42_RS00620 0.14 +0.3 162,545 + RR42_RS00620 0.14 -0.4 162,546 - RR42_RS00620 0.14 -0.0 162,546 - RR42_RS00620 0.14 -0.8 162,546 - RR42_RS00620 0.14 -1.3 162,546 - RR42_RS00620 0.14 +0.2 162,588 + RR42_RS00620 0.19 +0.5 162,588 + RR42_RS00620 0.19 -1.4 162,588 + RR42_RS00620 0.19 -1.3 162,849 + RR42_RS00620 0.46 +0.8 162,849 + RR42_RS00620 0.46 -0.4 162,849 + RR42_RS00620 0.46 +0.5 162,849 + RR42_RS00620 0.46 +1.6 162,850 - RR42_RS00620 0.46 -0.3 162,850 - RR42_RS00620 0.46 -0.5 162,850 - RR42_RS00620 0.46 +0.0 162,850 - RR42_RS00620 0.46 -0.1 162,850 - RR42_RS00620 0.46 -0.8
Or see this region's nucleotide sequence