Experiment: R2A with Dimethyl Sulfoxide 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS00030 and RR42_RS00035 are separated by 136 nucleotides RR42_RS00035 and RR42_RS00040 are separated by 213 nucleotides RR42_RS00040 and RR42_RS00045 overlap by 4 nucleotides
RR42_RS00030: RR42_RS00030 - hypothetical protein, at 10,744 to 13,032
_RS00030
RR42_RS00035: RR42_RS00035 - hypothetical protein, at 13,169 to 13,756
_RS00035
RR42_RS00040: RR42_RS00040 - recombinase, at 13,970 to 14,593
_RS00040
RR42_RS00045: RR42_RS00045 - lactate dehydrogenase, at 14,590 to 17,382
_RS00045
Position (kb) 13
14 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 12.313 kb on + strand, within RR42_RS00030 at 12.346 kb on + strand, within RR42_RS00030 at 12.346 kb on + strand, within RR42_RS00030 at 12.346 kb on + strand, within RR42_RS00030 at 12.346 kb on + strand, within RR42_RS00030 at 12.347 kb on - strand, within RR42_RS00030 at 12.390 kb on + strand, within RR42_RS00030 at 12.401 kb on - strand, within RR42_RS00030 at 12.401 kb on - strand, within RR42_RS00030 at 12.450 kb on + strand, within RR42_RS00030 at 12.450 kb on + strand, within RR42_RS00030 at 12.450 kb on + strand, within RR42_RS00030 at 12.463 kb on - strand, within RR42_RS00030 at 12.512 kb on + strand, within RR42_RS00030 at 12.512 kb on + strand, within RR42_RS00030 at 12.512 kb on + strand, within RR42_RS00030 at 12.512 kb on + strand, within RR42_RS00030 at 12.513 kb on - strand, within RR42_RS00030 at 12.565 kb on + strand, within RR42_RS00030 at 12.566 kb on - strand, within RR42_RS00030 at 12.566 kb on - strand, within RR42_RS00030 at 12.681 kb on + strand, within RR42_RS00030 at 12.681 kb on + strand, within RR42_RS00030 at 12.681 kb on + strand, within RR42_RS00030 at 12.682 kb on - strand, within RR42_RS00030 at 12.688 kb on + strand, within RR42_RS00030 at 12.689 kb on - strand, within RR42_RS00030 at 12.689 kb on - strand, within RR42_RS00030 at 12.689 kb on - strand, within RR42_RS00030 at 12.740 kb on + strand, within RR42_RS00030 at 12.741 kb on - strand, within RR42_RS00030 at 12.743 kb on + strand, within RR42_RS00030 at 12.744 kb on - strand, within RR42_RS00030 at 12.744 kb on - strand, within RR42_RS00030 at 12.744 kb on - strand, within RR42_RS00030 at 12.758 kb on + strand, within RR42_RS00030 at 12.758 kb on + strand, within RR42_RS00030 at 12.759 kb on - strand, within RR42_RS00030 at 12.759 kb on - strand, within RR42_RS00030 at 12.759 kb on - strand, within RR42_RS00030 at 12.759 kb on - strand, within RR42_RS00030 at 12.766 kb on + strand, within RR42_RS00030 at 12.821 kb on + strand at 12.824 kb on + strand at 12.825 kb on - strand at 12.874 kb on + strand at 12.874 kb on + strand at 12.874 kb on + strand at 12.875 kb on - strand at 12.884 kb on + strand at 12.884 kb on + strand at 12.885 kb on - strand at 12.885 kb on - strand at 12.885 kb on - strand at 12.954 kb on + strand at 12.973 kb on + strand at 12.973 kb on + strand at 12.973 kb on + strand at 12.974 kb on - strand at 13.079 kb on + strand at 13.080 kb on - strand at 13.127 kb on + strand at 13.127 kb on + strand at 13.128 kb on - strand at 13.128 kb on - strand at 13.583 kb on + strand, within RR42_RS00035 at 13.639 kb on + strand, within RR42_RS00035 at 13.640 kb on - strand, within RR42_RS00035 at 13.684 kb on - strand, within RR42_RS00035 at 13.686 kb on + strand, within RR42_RS00035 at 13.686 kb on + strand, within RR42_RS00035 at 13.686 kb on + strand, within RR42_RS00035 at 13.687 kb on - strand, within RR42_RS00035 at 13.792 kb on - strand at 13.792 kb on - strand at 13.879 kb on - strand at 13.879 kb on - strand at 13.879 kb on - strand at 13.901 kb on + strand at 13.901 kb on + strand at 13.902 kb on - strand at 13.902 kb on - strand at 13.902 kb on - strand at 13.902 kb on - strand at 14.067 kb on - strand, within RR42_RS00040 at 14.067 kb on - strand, within RR42_RS00040 at 14.130 kb on + strand, within RR42_RS00040 at 14.131 kb on - strand, within RR42_RS00040 at 14.131 kb on - strand, within RR42_RS00040 at 14.184 kb on + strand, within RR42_RS00040 at 14.184 kb on + strand, within RR42_RS00040 at 14.184 kb on + strand, within RR42_RS00040 at 14.185 kb on - strand, within RR42_RS00040 at 14.185 kb on - strand, within RR42_RS00040 at 14.504 kb on + strand, within RR42_RS00040 at 14.505 kb on - strand, within RR42_RS00040 at 14.573 kb on + strand at 14.574 kb on - strand at 14.575 kb on + strand at 14.601 kb on - strand at 14.617 kb on - strand at 14.617 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Dimethyl Sulfoxide 5 vol% remove 12,313 + RR42_RS00030 0.69 +0.1 12,346 + RR42_RS00030 0.70 -0.7 12,346 + RR42_RS00030 0.70 -0.5 12,346 + RR42_RS00030 0.70 +0.8 12,346 + RR42_RS00030 0.70 +0.2 12,347 - RR42_RS00030 0.70 -0.1 12,390 + RR42_RS00030 0.72 -0.8 12,401 - RR42_RS00030 0.72 -0.1 12,401 - RR42_RS00030 0.72 +0.8 12,450 + RR42_RS00030 0.75 -0.3 12,450 + RR42_RS00030 0.75 -0.1 12,450 + RR42_RS00030 0.75 -0.3 12,463 - RR42_RS00030 0.75 +0.0 12,512 + RR42_RS00030 0.77 -1.6 12,512 + RR42_RS00030 0.77 +0.1 12,512 + RR42_RS00030 0.77 +0.2 12,512 + RR42_RS00030 0.77 +1.1 12,513 - RR42_RS00030 0.77 -0.4 12,565 + RR42_RS00030 0.80 +0.9 12,566 - RR42_RS00030 0.80 -0.0 12,566 - RR42_RS00030 0.80 +0.7 12,681 + RR42_RS00030 0.85 -0.0 12,681 + RR42_RS00030 0.85 +1.1 12,681 + RR42_RS00030 0.85 +0.1 12,682 - RR42_RS00030 0.85 +0.5 12,688 + RR42_RS00030 0.85 +0.9 12,689 - RR42_RS00030 0.85 +0.6 12,689 - RR42_RS00030 0.85 +0.9 12,689 - RR42_RS00030 0.85 -0.4 12,740 + RR42_RS00030 0.87 -1.0 12,741 - RR42_RS00030 0.87 -0.6 12,743 + RR42_RS00030 0.87 +1.7 12,744 - RR42_RS00030 0.87 -0.1 12,744 - RR42_RS00030 0.87 +1.1 12,744 - RR42_RS00030 0.87 +0.2 12,758 + RR42_RS00030 0.88 +0.8 12,758 + RR42_RS00030 0.88 +0.5 12,759 - RR42_RS00030 0.88 +0.2 12,759 - RR42_RS00030 0.88 -0.1 12,759 - RR42_RS00030 0.88 -0.6 12,759 - RR42_RS00030 0.88 +0.9 12,766 + RR42_RS00030 0.88 +0.1 12,821 + +0.8 12,824 + -0.8 12,825 - +0.1 12,874 + +1.5 12,874 + +0.3 12,874 + +1.1 12,875 - +0.2 12,884 + -0.3 12,884 + +0.6 12,885 - -0.5 12,885 - -1.7 12,885 - -0.2 12,954 + +0.5 12,973 + -1.1 12,973 + -0.2 12,973 + +0.4 12,974 - -0.1 13,079 + -1.6 13,080 - +0.0 13,127 + +0.4 13,127 + -0.0 13,128 - +0.6 13,128 - +0.2 13,583 + RR42_RS00035 0.70 -0.3 13,639 + RR42_RS00035 0.80 -1.2 13,640 - RR42_RS00035 0.80 +0.2 13,684 - RR42_RS00035 0.88 -1.9 13,686 + RR42_RS00035 0.88 +1.5 13,686 + RR42_RS00035 0.88 -0.9 13,686 + RR42_RS00035 0.88 -3.2 13,687 - RR42_RS00035 0.88 +0.9 13,792 - +0.5 13,792 - +1.0 13,879 - -2.1 13,879 - +0.1 13,879 - +0.3 13,901 + -0.8 13,901 + +0.9 13,902 - -0.4 13,902 - -0.2 13,902 - +0.1 13,902 - -0.6 14,067 - RR42_RS00040 0.16 -0.9 14,067 - RR42_RS00040 0.16 -1.2 14,130 + RR42_RS00040 0.26 -0.7 14,131 - RR42_RS00040 0.26 -1.7 14,131 - RR42_RS00040 0.26 +0.8 14,184 + RR42_RS00040 0.34 +0.5 14,184 + RR42_RS00040 0.34 -0.6 14,184 + RR42_RS00040 0.34 -0.2 14,185 - RR42_RS00040 0.34 -0.7 14,185 - RR42_RS00040 0.34 +0.6 14,504 + RR42_RS00040 0.86 -0.1 14,505 - RR42_RS00040 0.86 -1.4 14,573 + +0.7 14,574 - -1.2 14,575 + -0.6 14,601 - +0.5 14,617 - +0.3 14,617 - -0.9
Or see this region's nucleotide sequence