Experiment: Formate with Nalidixic 6.25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS05170 and MMP_RS05175 overlap by 4 nucleotides MMP_RS05175 and MMP_RS05180 are separated by 213 nucleotides MMP_RS05180 and MMP_RS05185 are separated by 26 nucleotides MMP_RS05185 and MMP_RS05190 are separated by 0 nucleotides
MMP_RS05170: MMP_RS05170 - lysine exporter LysO family protein, at 984,278 to 984,865
_RS05170
MMP_RS05175: MMP_RS05175 - hypothetical protein, at 984,862 to 985,110
_RS05175
MMP_RS05180: MMP_RS05180 - Nre family DNA repair protein, at 985,324 to 986,544
_RS05180
MMP_RS05185: MMP_RS05185 - tryptophan synthase subunit alpha, at 986,571 to 987,347
_RS05185
MMP_RS05190: MMP_RS05190 - tryptophan synthase subunit beta, at 987,348 to 988,532
_RS05190
Position (kb)
985
986
987 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 984.362 kb on - strand, within MMP_RS05170 at 984.478 kb on - strand, within MMP_RS05170 at 984.479 kb on + strand, within MMP_RS05170 at 984.479 kb on + strand, within MMP_RS05170 at 984.540 kb on - strand, within MMP_RS05170 at 984.667 kb on - strand, within MMP_RS05170 at 984.667 kb on - strand, within MMP_RS05170 at 984.766 kb on + strand, within MMP_RS05170 at 984.766 kb on + strand, within MMP_RS05170 at 984.805 kb on - strand, within MMP_RS05170 at 984.820 kb on - strand at 984.821 kb on + strand at 984.873 kb on - strand at 984.906 kb on - strand, within MMP_RS05175 at 984.914 kb on + strand, within MMP_RS05175 at 984.940 kb on - strand, within MMP_RS05175 at 984.941 kb on + strand, within MMP_RS05175 at 984.941 kb on + strand, within MMP_RS05175 at 985.079 kb on + strand, within MMP_RS05175 at 985.096 kb on + strand at 985.097 kb on - strand at 985.098 kb on + strand at 985.098 kb on + strand at 985.110 kb on - strand at 985.142 kb on - strand at 985.158 kb on + strand at 985.159 kb on - strand at 985.162 kb on - strand at 985.162 kb on - strand at 985.164 kb on - strand at 985.165 kb on + strand at 985.165 kb on + strand at 985.166 kb on - strand at 985.167 kb on + strand at 985.175 kb on + strand at 985.175 kb on + strand at 985.186 kb on - strand at 985.187 kb on + strand at 985.189 kb on + strand at 985.244 kb on + strand at 985.244 kb on + strand at 985.247 kb on - strand at 985.247 kb on - strand at 985.247 kb on - strand at 985.247 kb on - strand at 985.251 kb on - strand at 985.252 kb on + strand at 985.254 kb on - strand at 985.254 kb on - strand at 985.323 kb on - strand at 985.400 kb on - strand at 985.401 kb on + strand at 985.446 kb on - strand, within MMP_RS05180 at 985.801 kb on + strand, within MMP_RS05180 at 985.852 kb on - strand, within MMP_RS05180 at 985.852 kb on - strand, within MMP_RS05180 at 985.921 kb on - strand, within MMP_RS05180 at 985.924 kb on - strand, within MMP_RS05180 at 985.925 kb on + strand, within MMP_RS05180 at 985.925 kb on + strand, within MMP_RS05180 at 985.994 kb on - strand, within MMP_RS05180 at 986.075 kb on + strand, within MMP_RS05180 at 986.097 kb on - strand, within MMP_RS05180 at 986.101 kb on - strand, within MMP_RS05180 at 986.102 kb on + strand, within MMP_RS05180 at 986.128 kb on - strand, within MMP_RS05180 at 986.270 kb on - strand, within MMP_RS05180 at 986.321 kb on - strand, within MMP_RS05180 at 986.321 kb on - strand, within MMP_RS05180 at 986.533 kb on + strand at 986.572 kb on - strand at 986.632 kb on + strand at 986.679 kb on - strand at 987.015 kb on + strand at 987.183 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Formate with Nalidixic 6.25 mM remove 984,362 - MMP_RS05170 0.14 +0.1 984,478 - MMP_RS05170 0.34 -0.1 984,479 + MMP_RS05170 0.34 -0.3 984,479 + MMP_RS05170 0.34 -0.4 984,540 - MMP_RS05170 0.45 +1.0 984,667 - MMP_RS05170 0.66 +0.2 984,667 - MMP_RS05170 0.66 +0.1 984,766 + MMP_RS05170 0.83 +0.3 984,766 + MMP_RS05170 0.83 +0.3 984,805 - MMP_RS05170 0.90 -0.0 984,820 - -0.0 984,821 + +0.2 984,873 - +0.1 984,906 - MMP_RS05175 0.18 +0.2 984,914 + MMP_RS05175 0.21 +0.9 984,940 - MMP_RS05175 0.31 -0.1 984,941 + MMP_RS05175 0.32 +0.3 984,941 + MMP_RS05175 0.32 +0.2 985,079 + MMP_RS05175 0.87 +0.3 985,096 + +0.1 985,097 - +0.3 985,098 + +0.1 985,098 + +0.1 985,110 - +0.1 985,142 - +0.2 985,158 + +0.5 985,159 - +0.5 985,162 - +0.2 985,162 - -0.1 985,164 - -0.1 985,165 + -0.1 985,165 + +0.2 985,166 - +0.0 985,167 + +0.1 985,175 + +0.2 985,175 + +0.2 985,186 - +0.2 985,187 + -0.2 985,189 + +0.4 985,244 + -0.2 985,244 + +0.2 985,247 - -0.7 985,247 - -0.7 985,247 - -0.1 985,247 - -0.1 985,251 - +0.8 985,252 + +0.3 985,254 - +0.2 985,254 - +0.9 985,323 - -0.1 985,400 - +0.7 985,401 + +0.1 985,446 - MMP_RS05180 0.10 +0.5 985,801 + MMP_RS05180 0.39 -0.1 985,852 - MMP_RS05180 0.43 +0.6 985,852 - MMP_RS05180 0.43 +0.7 985,921 - MMP_RS05180 0.49 -0.3 985,924 - MMP_RS05180 0.49 -0.2 985,925 + MMP_RS05180 0.49 +0.0 985,925 + MMP_RS05180 0.49 +0.5 985,994 - MMP_RS05180 0.55 +0.2 986,075 + MMP_RS05180 0.62 -0.2 986,097 - MMP_RS05180 0.63 +0.3 986,101 - MMP_RS05180 0.64 -0.1 986,102 + MMP_RS05180 0.64 +0.9 986,128 - MMP_RS05180 0.66 +0.1 986,270 - MMP_RS05180 0.77 +0.2 986,321 - MMP_RS05180 0.82 +0.2 986,321 - MMP_RS05180 0.82 -0.1 986,533 + +0.1 986,572 - +0.3 986,632 + -3.0 986,679 - -2.8 987,015 + -0.2 987,183 - +0.2
Or see this region's nucleotide sequence