Experiment: Formate with Nalidixic 6.25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS04175 and MMP_RS08975 are separated by 258 nucleotides MMP_RS08975 and MMP_RS04180 are separated by 196 nucleotides MMP_RS04180 and MMP_RS04185 are separated by 11 nucleotides
MMP_RS04175: MMP_RS04175 - permease, at 793,433 to 794,335
_RS04175
MMP_RS08975: MMP_RS08975 - hypothetical protein, at 794,594 to 794,749
_RS08975
MMP_RS04180: MMP_RS04180 - iron-containing alcohol dehydrogenase, at 794,946 to 796,115
_RS04180
MMP_RS04185: MMP_RS04185 - aldo/keto reductase, at 796,127 to 797,239
_RS04185
Position (kb)
794
795
796
797 Strain fitness (log2 ratio)
-1
0
1 at 793.967 kb on + strand, within MMP_RS04175 at 793.986 kb on + strand, within MMP_RS04175 at 794.027 kb on - strand, within MMP_RS04175 at 794.027 kb on - strand, within MMP_RS04175 at 794.028 kb on + strand, within MMP_RS04175 at 794.028 kb on + strand, within MMP_RS04175 at 794.113 kb on + strand, within MMP_RS04175 at 794.114 kb on - strand, within MMP_RS04175 at 794.131 kb on - strand, within MMP_RS04175 at 794.146 kb on - strand, within MMP_RS04175 at 794.147 kb on + strand, within MMP_RS04175 at 794.274 kb on + strand at 794.285 kb on + strand at 794.291 kb on + strand at 794.291 kb on + strand at 794.293 kb on + strand at 794.333 kb on - strand at 794.333 kb on - strand at 794.334 kb on + strand at 794.368 kb on - strand at 794.373 kb on + strand at 794.395 kb on + strand at 794.399 kb on + strand at 794.409 kb on - strand at 794.450 kb on - strand at 794.451 kb on + strand at 794.459 kb on - strand at 794.460 kb on + strand at 794.460 kb on + strand at 794.460 kb on + strand at 794.460 kb on + strand at 794.460 kb on + strand at 794.460 kb on + strand at 794.461 kb on - strand at 794.461 kb on - strand at 794.461 kb on - strand at 794.462 kb on + strand at 794.462 kb on + strand at 794.462 kb on + strand at 794.462 kb on + strand at 794.463 kb on - strand at 794.463 kb on - strand at 794.463 kb on - strand at 794.463 kb on - strand at 794.463 kb on - strand at 794.463 kb on - strand at 794.464 kb on + strand at 794.464 kb on + strand at 794.464 kb on + strand at 794.465 kb on - strand at 794.465 kb on - strand at 794.469 kb on + strand at 794.518 kb on + strand at 794.590 kb on - strand at 794.597 kb on - strand at 794.597 kb on - strand at 794.597 kb on - strand at 794.597 kb on - strand at 794.598 kb on + strand at 794.598 kb on + strand at 794.606 kb on - strand at 794.644 kb on + strand, within MMP_RS08975 at 794.668 kb on + strand, within MMP_RS08975 at 794.690 kb on + strand, within MMP_RS08975 at 794.690 kb on + strand, within MMP_RS08975 at 794.819 kb on - strand at 794.840 kb on - strand at 794.841 kb on + strand at 794.844 kb on - strand at 794.849 kb on - strand at 794.850 kb on + strand at 794.850 kb on + strand at 794.850 kb on + strand at 794.850 kb on + strand at 794.851 kb on - strand at 794.851 kb on - strand at 794.852 kb on + strand at 794.853 kb on - strand at 794.853 kb on - strand at 794.853 kb on - strand at 794.853 kb on - strand at 794.853 kb on - strand at 794.853 kb on - strand at 794.854 kb on + strand at 794.854 kb on + strand at 794.854 kb on + strand at 794.854 kb on + strand at 794.854 kb on + strand at 794.854 kb on + strand at 794.854 kb on + strand at 794.854 kb on + strand at 794.854 kb on + strand at 794.854 kb on + strand at 794.856 kb on + strand at 794.908 kb on - strand at 794.908 kb on - strand at 794.911 kb on - strand at 794.945 kb on - strand at 794.961 kb on - strand at 794.961 kb on - strand at 795.122 kb on + strand, within MMP_RS04180 at 795.133 kb on - strand, within MMP_RS04180 at 795.134 kb on + strand, within MMP_RS04180 at 795.371 kb on - strand, within MMP_RS04180 at 795.372 kb on + strand, within MMP_RS04180 at 795.565 kb on + strand, within MMP_RS04180 at 795.565 kb on + strand, within MMP_RS04180 at 795.568 kb on - strand, within MMP_RS04180 at 795.600 kb on - strand, within MMP_RS04180 at 795.600 kb on - strand, within MMP_RS04180 at 795.600 kb on - strand, within MMP_RS04180 at 795.601 kb on + strand, within MMP_RS04180 at 795.601 kb on + strand, within MMP_RS04180 at 795.850 kb on - strand, within MMP_RS04180 at 795.851 kb on + strand, within MMP_RS04180 at 795.883 kb on - strand, within MMP_RS04180 at 795.940 kb on - strand, within MMP_RS04180 at 795.968 kb on - strand, within MMP_RS04180 at 795.969 kb on + strand, within MMP_RS04180 at 795.982 kb on + strand, within MMP_RS04180 at 795.989 kb on - strand, within MMP_RS04180 at 796.050 kb on + strand at 796.069 kb on - strand at 796.070 kb on + strand at 796.108 kb on + strand at 796.169 kb on + strand at 796.236 kb on + strand at 796.278 kb on + strand, within MMP_RS04185 at 796.304 kb on + strand, within MMP_RS04185 at 796.333 kb on - strand, within MMP_RS04185 at 796.333 kb on - strand, within MMP_RS04185 at 796.347 kb on - strand, within MMP_RS04185 at 796.347 kb on - strand, within MMP_RS04185 at 796.445 kb on + strand, within MMP_RS04185 at 796.448 kb on + strand, within MMP_RS04185 at 796.600 kb on - strand, within MMP_RS04185 at 796.635 kb on - strand, within MMP_RS04185 at 796.861 kb on + strand, within MMP_RS04185 at 796.881 kb on - strand, within MMP_RS04185 at 796.916 kb on - strand, within MMP_RS04185 at 796.919 kb on - strand, within MMP_RS04185 at 797.002 kb on - strand, within MMP_RS04185 at 797.047 kb on + strand, within MMP_RS04185 at 797.055 kb on + strand, within MMP_RS04185 at 797.099 kb on - strand, within MMP_RS04185 at 797.100 kb on + strand, within MMP_RS04185
Per-strain Table
Position Strand Gene LocusTag Fraction Formate with Nalidixic 6.25 mM remove 793,967 + MMP_RS04175 0.59 +0.1 793,986 + MMP_RS04175 0.61 -0.2 794,027 - MMP_RS04175 0.66 -0.0 794,027 - MMP_RS04175 0.66 +0.4 794,028 + MMP_RS04175 0.66 +0.1 794,028 + MMP_RS04175 0.66 -0.3 794,113 + MMP_RS04175 0.75 +0.1 794,114 - MMP_RS04175 0.75 +0.2 794,131 - MMP_RS04175 0.77 -0.0 794,146 - MMP_RS04175 0.79 -0.1 794,147 + MMP_RS04175 0.79 -0.2 794,274 + -0.0 794,285 + -0.6 794,291 + -0.4 794,291 + -0.0 794,293 + +0.1 794,333 - -0.3 794,333 - -0.1 794,334 + +0.2 794,368 - -0.1 794,373 + -0.2 794,395 + -0.5 794,399 + +0.1 794,409 - -0.1 794,450 - +0.1 794,451 + +0.3 794,459 - -0.2 794,460 + +0.2 794,460 + +0.2 794,460 + +0.3 794,460 + +0.4 794,460 + +0.3 794,460 + -0.5 794,461 - +0.2 794,461 - +0.3 794,461 - +0.0 794,462 + +0.4 794,462 + +0.6 794,462 + -0.4 794,462 + +0.4 794,463 - -0.0 794,463 - +0.0 794,463 - -0.0 794,463 - +0.2 794,463 - -0.0 794,463 - +0.0 794,464 + -0.7 794,464 + +0.0 794,464 + +0.8 794,465 - +0.6 794,465 - +0.5 794,469 + +0.0 794,518 + -0.2 794,590 - -0.6 794,597 - +0.3 794,597 - -0.2 794,597 - +0.2 794,597 - -0.3 794,598 + +0.3 794,598 + +0.1 794,606 - +0.7 794,644 + MMP_RS08975 0.32 -0.0 794,668 + MMP_RS08975 0.47 +0.2 794,690 + MMP_RS08975 0.62 -0.3 794,690 + MMP_RS08975 0.62 +0.0 794,819 - -0.6 794,840 - +0.4 794,841 + -0.0 794,844 - +0.0 794,849 - +0.0 794,850 + -0.0 794,850 + +0.3 794,850 + +0.2 794,850 + +0.2 794,851 - -0.3 794,851 - +0.2 794,852 + +0.1 794,853 - -0.1 794,853 - +0.3 794,853 - -0.2 794,853 - -0.1 794,853 - +0.0 794,853 - -0.3 794,854 + -0.2 794,854 + -0.2 794,854 + -0.5 794,854 + -0.2 794,854 + +0.2 794,854 + +0.3 794,854 + -0.4 794,854 + +0.4 794,854 + -0.3 794,854 + +0.1 794,856 + -0.2 794,908 - +0.4 794,908 - +0.3 794,911 - +0.1 794,945 - -0.1 794,961 - +0.2 794,961 - +0.4 795,122 + MMP_RS04180 0.15 +0.5 795,133 - MMP_RS04180 0.16 +0.2 795,134 + MMP_RS04180 0.16 -0.1 795,371 - MMP_RS04180 0.36 +0.0 795,372 + MMP_RS04180 0.36 +0.5 795,565 + MMP_RS04180 0.53 +0.4 795,565 + MMP_RS04180 0.53 +0.7 795,568 - MMP_RS04180 0.53 +0.1 795,600 - MMP_RS04180 0.56 -0.2 795,600 - MMP_RS04180 0.56 +0.2 795,600 - MMP_RS04180 0.56 -0.3 795,601 + MMP_RS04180 0.56 +0.1 795,601 + MMP_RS04180 0.56 +0.5 795,850 - MMP_RS04180 0.77 +0.4 795,851 + MMP_RS04180 0.77 -0.4 795,883 - MMP_RS04180 0.80 +0.3 795,940 - MMP_RS04180 0.85 -0.0 795,968 - MMP_RS04180 0.87 +0.2 795,969 + MMP_RS04180 0.87 +0.2 795,982 + MMP_RS04180 0.89 +0.2 795,989 - MMP_RS04180 0.89 +0.2 796,050 + +0.1 796,069 - -0.2 796,070 + -0.7 796,108 + +0.2 796,169 + +0.2 796,236 + -0.4 796,278 + MMP_RS04185 0.14 -0.1 796,304 + MMP_RS04185 0.16 -0.3 796,333 - MMP_RS04185 0.19 +0.2 796,333 - MMP_RS04185 0.19 +0.2 796,347 - MMP_RS04185 0.20 +0.1 796,347 - MMP_RS04185 0.20 -0.6 796,445 + MMP_RS04185 0.29 -0.0 796,448 + MMP_RS04185 0.29 -0.1 796,600 - MMP_RS04185 0.42 -0.4 796,635 - MMP_RS04185 0.46 -0.1 796,861 + MMP_RS04185 0.66 +0.1 796,881 - MMP_RS04185 0.68 -0.1 796,916 - MMP_RS04185 0.71 +0.3 796,919 - MMP_RS04185 0.71 +0.6 797,002 - MMP_RS04185 0.79 +0.2 797,047 + MMP_RS04185 0.83 -0.4 797,055 + MMP_RS04185 0.83 +0.6 797,099 - MMP_RS04185 0.87 +0.1 797,100 + MMP_RS04185 0.87 -0.4
Or see this region's nucleotide sequence