Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS20160

Experiment: D-Lactate (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS20150 and BWI76_RS20155 are separated by 10 nucleotidesBWI76_RS20155 and BWI76_RS20160 are separated by 14 nucleotidesBWI76_RS20160 and BWI76_RS20165 overlap by 4 nucleotides BWI76_RS20150: BWI76_RS20150 - NADH:ubiquinone oxidoreductase subunit J, at 4,222,275 to 4,222,829 _RS20150 BWI76_RS20155: BWI76_RS20155 - NADH-quinone oxidoreductase subunit I, at 4,222,840 to 4,223,382 _RS20155 BWI76_RS20160: BWI76_RS20160 - NADH-quinone oxidoreductase subunit H, at 4,223,397 to 4,224,374 _RS20160 BWI76_RS20165: BWI76_RS20165 - NADH-quinone oxidoreductase subunit G, at 4,224,371 to 4,227,097 _RS20165 Position (kb) 4223 4224 4225Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 4222.604 kb on + strand, within BWI76_RS20150at 4222.645 kb on - strand, within BWI76_RS20150at 4222.756 kb on + strand, within BWI76_RS20150at 4222.891 kb on + strandat 4222.899 kb on - strand, within BWI76_RS20155at 4223.157 kb on + strand, within BWI76_RS20155at 4223.190 kb on + strand, within BWI76_RS20155at 4223.388 kb on + strandat 4223.447 kb on + strandat 4223.659 kb on + strand, within BWI76_RS20160at 4223.804 kb on - strand, within BWI76_RS20160at 4224.021 kb on + strand, within BWI76_RS20160at 4224.218 kb on - strand, within BWI76_RS20160at 4224.587 kb on - strandat 4225.133 kb on - strand, within BWI76_RS20165

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Lactate (C)
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4,222,604 + BWI76_RS20150 0.59 +0.8
4,222,645 - BWI76_RS20150 0.67 -2.9
4,222,756 + BWI76_RS20150 0.87 -1.9
4,222,891 + -1.4
4,222,899 - BWI76_RS20155 0.11 -1.6
4,223,157 + BWI76_RS20155 0.58 -1.4
4,223,190 + BWI76_RS20155 0.64 -1.1
4,223,388 + -1.6
4,223,447 + -2.6
4,223,659 + BWI76_RS20160 0.27 -4.3
4,223,804 - BWI76_RS20160 0.42 -1.8
4,224,021 + BWI76_RS20160 0.64 +0.2
4,224,218 - BWI76_RS20160 0.84 -3.3
4,224,587 - -3.1
4,225,133 - BWI76_RS20165 0.28 -0.6

Or see this region's nucleotide sequence