Experiment: D-Lactate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS20150 and BWI76_RS20155 are separated by 10 nucleotides BWI76_RS20155 and BWI76_RS20160 are separated by 14 nucleotides BWI76_RS20160 and BWI76_RS20165 overlap by 4 nucleotides
BWI76_RS20150: BWI76_RS20150 - NADH:ubiquinone oxidoreductase subunit J, at 4,222,275 to 4,222,829
_RS20150
BWI76_RS20155: BWI76_RS20155 - NADH-quinone oxidoreductase subunit I, at 4,222,840 to 4,223,382
_RS20155
BWI76_RS20160: BWI76_RS20160 - NADH-quinone oxidoreductase subunit H, at 4,223,397 to 4,224,374
_RS20160
BWI76_RS20165: BWI76_RS20165 - NADH-quinone oxidoreductase subunit G, at 4,224,371 to 4,227,097
_RS20165
Position (kb)
4223
4224
4225 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 4222.604 kb on + strand, within BWI76_RS20150 at 4222.645 kb on - strand, within BWI76_RS20150 at 4222.756 kb on + strand, within BWI76_RS20150 at 4222.891 kb on + strand at 4222.899 kb on - strand, within BWI76_RS20155 at 4223.157 kb on + strand, within BWI76_RS20155 at 4223.190 kb on + strand, within BWI76_RS20155 at 4223.388 kb on + strand at 4223.447 kb on + strand at 4223.659 kb on + strand, within BWI76_RS20160 at 4223.804 kb on - strand, within BWI76_RS20160 at 4224.021 kb on + strand, within BWI76_RS20160 at 4224.218 kb on - strand, within BWI76_RS20160 at 4224.587 kb on - strand at 4225.133 kb on - strand, within BWI76_RS20165
Per-strain Table
Position Strand Gene LocusTag Fraction D-Lactate (C) remove 4,222,604 + BWI76_RS20150 0.59 +0.8 4,222,645 - BWI76_RS20150 0.67 -2.9 4,222,756 + BWI76_RS20150 0.87 -1.9 4,222,891 + -1.4 4,222,899 - BWI76_RS20155 0.11 -1.6 4,223,157 + BWI76_RS20155 0.58 -1.4 4,223,190 + BWI76_RS20155 0.64 -1.1 4,223,388 + -1.6 4,223,447 + -2.6 4,223,659 + BWI76_RS20160 0.27 -4.3 4,223,804 - BWI76_RS20160 0.42 -1.8 4,224,021 + BWI76_RS20160 0.64 +0.2 4,224,218 - BWI76_RS20160 0.84 -3.3 4,224,587 - -3.1 4,225,133 - BWI76_RS20165 0.28 -0.6
Or see this region's nucleotide sequence