Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS18325

Experiment: D-Lactate (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS18305 and BWI76_RS18310 are separated by 190 nucleotidesBWI76_RS18310 and BWI76_RS18315 are separated by 13 nucleotidesBWI76_RS18315 and BWI76_RS18320 are separated by 38 nucleotidesBWI76_RS18320 and BWI76_RS18325 are separated by 151 nucleotidesBWI76_RS18325 and BWI76_RS18330 are separated by 57 nucleotides BWI76_RS18305: BWI76_RS18305 - dihydrodipicolinate synthase family protein, at 3,803,453 to 3,804,340 _RS18305 BWI76_RS18310: BWI76_RS18310 - tRNA-Leu, at 3,804,531 to 3,804,617 _RS18310 BWI76_RS18315: BWI76_RS18315 - tRNA-Cys, at 3,804,631 to 3,804,704 _RS18315 BWI76_RS18320: BWI76_RS18320 - tRNA-Gly, at 3,804,743 to 3,804,818 _RS18320 BWI76_RS18325: BWI76_RS18325 - CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase, at 3,804,970 to 3,805,518 _RS18325 BWI76_RS18330: BWI76_RS18330 - excinuclease ABC subunit C, at 3,805,576 to 3,807,408 _RS18330 Position (kb) 3804 3805 3806Strain fitness (log2 ratio) -1 0 1at 3803.975 kb on - strand, within BWI76_RS18305at 3804.128 kb on + strand, within BWI76_RS18305at 3805.581 kb on + strandat 3805.828 kb on - strand, within BWI76_RS18330at 3805.859 kb on - strand, within BWI76_RS18330at 3805.859 kb on - strand, within BWI76_RS18330at 3806.088 kb on + strand, within BWI76_RS18330

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Lactate (C)
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3,803,975 - BWI76_RS18305 0.59 -0.1
3,804,128 + BWI76_RS18305 0.76 -1.0
3,805,581 + +0.1
3,805,828 - BWI76_RS18330 0.14 +0.6
3,805,859 - BWI76_RS18330 0.15 -0.0
3,805,859 - BWI76_RS18330 0.15 -0.3
3,806,088 + BWI76_RS18330 0.28 +0.4

Or see this region's nucleotide sequence