Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS04880

Experiment: D-Lactate (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS04875 and BWI76_RS04880 are separated by 119 nucleotidesBWI76_RS04880 and BWI76_RS04885 are separated by 14 nucleotides BWI76_RS04875: BWI76_RS04875 - transcriptional regulator PdhR, at 1,019,107 to 1,019,886 _RS04875 BWI76_RS04880: BWI76_RS04880 - pyruvate dehydrogenase (acetyl-transferring), homodimeric type, at 1,020,006 to 1,022,669 _RS04880 BWI76_RS04885: BWI76_RS04885 - pyruvate dehydrogenase complex dihydrolipoyllysine-residue acetyltransferase, at 1,022,684 to 1,024,573 _RS04885 Position (kb) 1020 1021 1022 1023Strain fitness (log2 ratio) -1 0 1 2at 1019.425 kb on + strand, within BWI76_RS04875at 1019.425 kb on + strand, within BWI76_RS04875at 1019.433 kb on - strand, within BWI76_RS04875at 1019.621 kb on + strand, within BWI76_RS04875at 1019.818 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Lactate (C)
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1,019,425 + BWI76_RS04875 0.41 +1.8
1,019,425 + BWI76_RS04875 0.41 +1.9
1,019,433 - BWI76_RS04875 0.42 -1.6
1,019,621 + BWI76_RS04875 0.66 +0.9
1,019,818 + +2.0

Or see this region's nucleotide sequence