Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO353_20660 and AO353_20665 are separated by 63 nucleotides AO353_20665 and AO353_20670 are separated by 218 nucleotides AO353_20670 and AO353_20675 overlap by 4 nucleotides
AO353_20660: AO353_20660 - pseudouridine synthase, at 4,533,888 to 4,534,712
_20660
AO353_20665: AO353_20665 - N-(5'-phosphoribosyl)anthranilate isomerase, at 4,534,776 to 4,535,399
_20665
AO353_20670: AO353_20670 - acetyl-CoA carboxylase subunit beta, at 4,535,618 to 4,536,538
_20670
AO353_20675: AO353_20675 - bifunctional folylpolyglutamate synthase/dihydrofolate synthase, at 4,536,535 to 4,537,842
_20675
Position (kb)
4535
4536
4537 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 4534.618 kb on - strand, within AO353_20660 at 4534.618 kb on - strand, within AO353_20660 at 4534.618 kb on - strand, within AO353_20660 at 4534.618 kb on - strand, within AO353_20660 at 4534.635 kb on + strand at 4534.665 kb on + strand at 4534.665 kb on + strand at 4534.665 kb on + strand at 4534.666 kb on - strand at 4534.666 kb on - strand at 4534.666 kb on - strand at 4534.666 kb on - strand at 4534.666 kb on - strand at 4534.872 kb on + strand, within AO353_20665 at 4534.872 kb on + strand, within AO353_20665 at 4534.872 kb on + strand, within AO353_20665 at 4534.872 kb on + strand, within AO353_20665 at 4534.872 kb on + strand, within AO353_20665 at 4534.873 kb on - strand, within AO353_20665 at 4534.873 kb on - strand, within AO353_20665 at 4534.873 kb on - strand, within AO353_20665 at 4534.873 kb on - strand, within AO353_20665 at 4535.062 kb on - strand, within AO353_20665 at 4535.073 kb on + strand, within AO353_20665 at 4535.073 kb on + strand, within AO353_20665 at 4535.073 kb on + strand, within AO353_20665 at 4535.073 kb on + strand, within AO353_20665 at 4535.073 kb on + strand, within AO353_20665 at 4535.073 kb on + strand, within AO353_20665 at 4535.073 kb on + strand, within AO353_20665 at 4535.074 kb on - strand, within AO353_20665 at 4535.074 kb on - strand, within AO353_20665 at 4535.074 kb on - strand, within AO353_20665 at 4535.074 kb on - strand, within AO353_20665 at 4535.074 kb on - strand, within AO353_20665 at 4535.077 kb on + strand, within AO353_20665 at 4535.077 kb on + strand, within AO353_20665 at 4535.077 kb on + strand, within AO353_20665 at 4535.077 kb on + strand, within AO353_20665 at 4535.077 kb on + strand, within AO353_20665 at 4535.077 kb on + strand, within AO353_20665 at 4535.077 kb on + strand, within AO353_20665 at 4535.078 kb on - strand, within AO353_20665 at 4535.078 kb on - strand, within AO353_20665 at 4535.160 kb on - strand, within AO353_20665 at 4535.160 kb on - strand, within AO353_20665 at 4535.160 kb on - strand, within AO353_20665 at 4535.163 kb on + strand, within AO353_20665 at 4535.163 kb on + strand, within AO353_20665 at 4535.163 kb on + strand, within AO353_20665 at 4535.163 kb on + strand, within AO353_20665 at 4535.163 kb on + strand, within AO353_20665 at 4535.163 kb on + strand, within AO353_20665 at 4535.163 kb on + strand, within AO353_20665 at 4535.163 kb on + strand, within AO353_20665 at 4535.163 kb on + strand, within AO353_20665 at 4535.164 kb on - strand, within AO353_20665 at 4535.164 kb on - strand, within AO353_20665 at 4535.164 kb on - strand, within AO353_20665 at 4535.164 kb on - strand, within AO353_20665 at 4535.164 kb on - strand, within AO353_20665 at 4535.164 kb on - strand, within AO353_20665 at 4535.164 kb on - strand, within AO353_20665 at 4535.164 kb on - strand, within AO353_20665 at 4535.164 kb on - strand, within AO353_20665 at 4535.164 kb on - strand, within AO353_20665 at 4535.326 kb on + strand, within AO353_20665 at 4535.374 kb on + strand at 4535.374 kb on + strand at 4535.374 kb on + strand at 4535.374 kb on + strand at 4535.390 kb on + strand at 4535.390 kb on + strand at 4535.397 kb on + strand at 4535.441 kb on + strand at 4535.454 kb on + strand at 4535.454 kb on + strand at 4535.454 kb on + strand at 4535.454 kb on + strand at 4535.454 kb on + strand at 4535.454 kb on + strand at 4535.454 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 4,534,618 - AO353_20660 0.88 -1.2 4,534,618 - AO353_20660 0.88 -0.6 4,534,618 - AO353_20660 0.88 -3.2 4,534,618 - AO353_20660 0.88 -1.5 4,534,635 + -0.4 4,534,665 + -0.7 4,534,665 + -1.0 4,534,665 + -1.3 4,534,666 - +0.0 4,534,666 - -1.2 4,534,666 - -2.1 4,534,666 - -1.5 4,534,666 - -2.0 4,534,872 + AO353_20665 0.15 -2.1 4,534,872 + AO353_20665 0.15 +0.3 4,534,872 + AO353_20665 0.15 -0.6 4,534,872 + AO353_20665 0.15 -1.3 4,534,872 + AO353_20665 0.15 -2.4 4,534,873 - AO353_20665 0.16 -0.7 4,534,873 - AO353_20665 0.16 -2.3 4,534,873 - AO353_20665 0.16 -1.1 4,534,873 - AO353_20665 0.16 -2.3 4,535,062 - AO353_20665 0.46 -1.1 4,535,073 + AO353_20665 0.48 -1.6 4,535,073 + AO353_20665 0.48 -1.7 4,535,073 + AO353_20665 0.48 -1.1 4,535,073 + AO353_20665 0.48 -2.4 4,535,073 + AO353_20665 0.48 -1.7 4,535,073 + AO353_20665 0.48 -1.6 4,535,073 + AO353_20665 0.48 -0.7 4,535,074 - AO353_20665 0.48 -2.3 4,535,074 - AO353_20665 0.48 -2.1 4,535,074 - AO353_20665 0.48 -2.2 4,535,074 - AO353_20665 0.48 -1.2 4,535,074 - AO353_20665 0.48 -0.4 4,535,077 + AO353_20665 0.48 -1.4 4,535,077 + AO353_20665 0.48 -1.8 4,535,077 + AO353_20665 0.48 -2.6 4,535,077 + AO353_20665 0.48 -1.5 4,535,077 + AO353_20665 0.48 -1.0 4,535,077 + AO353_20665 0.48 -1.4 4,535,077 + AO353_20665 0.48 -0.8 4,535,078 - AO353_20665 0.48 -0.7 4,535,078 - AO353_20665 0.48 -1.9 4,535,160 - AO353_20665 0.62 -1.0 4,535,160 - AO353_20665 0.62 -2.7 4,535,160 - AO353_20665 0.62 -1.3 4,535,163 + AO353_20665 0.62 -0.4 4,535,163 + AO353_20665 0.62 -1.3 4,535,163 + AO353_20665 0.62 -2.0 4,535,163 + AO353_20665 0.62 -3.1 4,535,163 + AO353_20665 0.62 -0.7 4,535,163 + AO353_20665 0.62 +0.0 4,535,163 + AO353_20665 0.62 -2.0 4,535,163 + AO353_20665 0.62 -2.3 4,535,163 + AO353_20665 0.62 -1.5 4,535,164 - AO353_20665 0.62 -1.0 4,535,164 - AO353_20665 0.62 -1.8 4,535,164 - AO353_20665 0.62 -2.1 4,535,164 - AO353_20665 0.62 -0.8 4,535,164 - AO353_20665 0.62 -2.9 4,535,164 - AO353_20665 0.62 -2.3 4,535,164 - AO353_20665 0.62 -2.9 4,535,164 - AO353_20665 0.62 -0.4 4,535,164 - AO353_20665 0.62 +0.2 4,535,164 - AO353_20665 0.62 -3.2 4,535,326 + AO353_20665 0.88 -1.7 4,535,374 + +0.3 4,535,374 + -0.6 4,535,374 + -2.3 4,535,374 + -1.5 4,535,390 + +0.7 4,535,390 + +0.3 4,535,397 + -0.1 4,535,441 + -0.3 4,535,454 + -0.6 4,535,454 + -0.6 4,535,454 + +1.4 4,535,454 + -0.8 4,535,454 + +0.0 4,535,454 + -0.5 4,535,454 + -1.2
Or see this region's nucleotide sequence