Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO353_16470 and AO353_16475 overlap by 4 nucleotides AO353_16475 and AO353_16480 are separated by 17 nucleotides AO353_16480 and AO353_16485 overlap by 1 nucleotides AO353_16485 and AO353_16490 overlap by 4 nucleotides
AO353_16470: AO353_16470 - uridylate kinase, at 3,657,780 to 3,658,523
_16470
AO353_16475: AO353_16475 - ribosome-recycling factor, at 3,658,520 to 3,659,077
_16475
AO353_16480: AO353_16480 - farnesyl-diphosphate synthase, at 3,659,095 to 3,659,850
_16480
AO353_16485: AO353_16485 - phosphatidate cytidylyltransferase, at 3,659,850 to 3,660,656
_16485
AO353_16490: AO353_16490 - 1-deoxy-D-xylulose 5-phosphate reductoisomerase, at 3,660,653 to 3,661,843
_16490
Position (kb)
3659
3660 Strain fitness (log2 ratio)
-1
0
1 at 3659.073 kb on + strand at 3659.073 kb on + strand at 3659.073 kb on + strand at 3659.073 kb on + strand at 3659.073 kb on + strand at 3659.073 kb on + strand at 3659.073 kb on + strand at 3659.073 kb on + strand at 3659.073 kb on + strand at 3659.073 kb on + strand at 3659.073 kb on + strand at 3659.073 kb on + strand at 3659.073 kb on + strand at 3659.075 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 3,659,073 + -0.4 3,659,073 + +1.0 3,659,073 + +0.9 3,659,073 + +1.4 3,659,073 + -1.4 3,659,073 + +1.6 3,659,073 + -0.3 3,659,073 + +0.5 3,659,073 + -1.0 3,659,073 + +0.4 3,659,073 + -0.2 3,659,073 + -0.9 3,659,073 + +0.2 3,659,075 + -1.1
Or see this region's nucleotide sequence