Experiment: R2A with Bacitracin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS36520 and RR42_RS36525 are separated by 146 nucleotides RR42_RS36525 and RR42_RS36530 are separated by 36 nucleotides RR42_RS36530 and RR42_RS36535 are separated by 277 nucleotides RR42_RS36535 and RR42_RS36540 are separated by 84 nucleotides
RR42_RS36520: RR42_RS36520 - AraC family transcriptional regulator, at 3,650,678 to 3,651,814
_RS36520
RR42_RS36525: RR42_RS36525 - universal stress protein UspA, at 3,651,961 to 3,652,395
_RS36525
RR42_RS36530: RR42_RS36530 - hypothetical protein, at 3,652,432 to 3,652,650
_RS36530
RR42_RS36535: RR42_RS36535 - hypothetical protein, at 3,652,928 to 3,653,176
_RS36535
RR42_RS36540: RR42_RS36540 - CoA-transferase, at 3,653,261 to 3,654,484
_RS36540
Position (kb)
3652
3653 Strain fitness (log2 ratio)
-2
-1
0
1 at 3651.985 kb on - strand at 3651.985 kb on - strand at 3651.990 kb on + strand at 3651.991 kb on - strand at 3651.991 kb on - strand at 3651.991 kb on - strand at 3652.289 kb on + strand, within RR42_RS36525 at 3652.289 kb on + strand, within RR42_RS36525 at 3652.289 kb on + strand, within RR42_RS36525 at 3652.289 kb on + strand, within RR42_RS36525 at 3652.290 kb on - strand, within RR42_RS36525 at 3652.290 kb on - strand, within RR42_RS36525 at 3652.290 kb on - strand, within RR42_RS36525 at 3652.463 kb on + strand, within RR42_RS36530 at 3652.464 kb on - strand, within RR42_RS36530 at 3652.624 kb on + strand, within RR42_RS36530 at 3652.625 kb on - strand, within RR42_RS36530 at 3652.728 kb on + strand at 3652.728 kb on + strand at 3652.729 kb on - strand at 3652.859 kb on + strand at 3652.866 kb on + strand at 3652.893 kb on - strand at 3652.895 kb on - strand at 3653.244 kb on + strand at 3653.443 kb on + strand, within RR42_RS36540 at 3653.464 kb on - strand, within RR42_RS36540 at 3653.496 kb on + strand, within RR42_RS36540 at 3653.497 kb on - strand, within RR42_RS36540 at 3653.573 kb on - strand, within RR42_RS36540
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Bacitracin 0.5 mg/ml remove 3,651,985 - +0.1 3,651,985 - -1.8 3,651,990 + +0.0 3,651,991 - -0.9 3,651,991 - -0.9 3,651,991 - +0.2 3,652,289 + RR42_RS36525 0.75 -0.4 3,652,289 + RR42_RS36525 0.75 +0.6 3,652,289 + RR42_RS36525 0.75 -0.0 3,652,289 + RR42_RS36525 0.75 -2.6 3,652,290 - RR42_RS36525 0.76 -0.4 3,652,290 - RR42_RS36525 0.76 -0.5 3,652,290 - RR42_RS36525 0.76 -0.5 3,652,463 + RR42_RS36530 0.14 -0.9 3,652,464 - RR42_RS36530 0.15 -0.4 3,652,624 + RR42_RS36530 0.88 -0.7 3,652,625 - RR42_RS36530 0.88 +0.3 3,652,728 + +1.5 3,652,728 + -0.6 3,652,729 - +0.2 3,652,859 + -1.5 3,652,866 + -0.4 3,652,893 - -0.4 3,652,895 - -0.1 3,653,244 + +0.0 3,653,443 + RR42_RS36540 0.15 +1.4 3,653,464 - RR42_RS36540 0.17 -1.1 3,653,496 + RR42_RS36540 0.19 +0.2 3,653,497 - RR42_RS36540 0.19 -1.3 3,653,573 - RR42_RS36540 0.25 +0.8
Or see this region's nucleotide sequence