Experiment: R2A with Bacitracin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS34270 and RR42_RS34275 are separated by 216 nucleotides RR42_RS34275 and RR42_RS34280 are separated by 25 nucleotides RR42_RS34280 and RR42_RS34285 are separated by 219 nucleotides
RR42_RS34270: RR42_RS34270 - Fis family transcriptional regulator, at 3,116,377 to 3,118,302
_RS34270
RR42_RS34275: RR42_RS34275 - virulence protein, at 3,118,519 to 3,118,926
_RS34275
RR42_RS34280: RR42_RS34280 - hypothetical protein, at 3,118,952 to 3,119,224
_RS34280
RR42_RS34285: RR42_RS34285 - alpha/beta hydrolase, at 3,119,444 to 3,120,400
_RS34285
Position (kb)
3118
3119
3120 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3118.121 kb on + strand at 3118.121 kb on + strand at 3118.121 kb on + strand at 3118.121 kb on + strand at 3118.121 kb on + strand at 3118.122 kb on - strand at 3118.122 kb on - strand at 3118.122 kb on - strand at 3118.122 kb on - strand at 3118.179 kb on - strand at 3118.399 kb on + strand at 3118.400 kb on - strand at 3118.449 kb on - strand at 3118.449 kb on - strand at 3118.532 kb on + strand at 3118.548 kb on + strand at 3118.548 kb on + strand at 3118.549 kb on - strand at 3118.549 kb on - strand at 3118.759 kb on - strand, within RR42_RS34275 at 3118.870 kb on + strand, within RR42_RS34275 at 3118.870 kb on + strand, within RR42_RS34275 at 3118.870 kb on + strand, within RR42_RS34275 at 3118.871 kb on - strand, within RR42_RS34275 at 3119.174 kb on + strand, within RR42_RS34280 at 3119.174 kb on + strand, within RR42_RS34280 at 3119.175 kb on - strand, within RR42_RS34280 at 3119.175 kb on - strand, within RR42_RS34280 at 3119.175 kb on - strand, within RR42_RS34280 at 3119.273 kb on + strand at 3119.273 kb on + strand at 3119.274 kb on - strand at 3119.274 kb on - strand at 3119.278 kb on + strand at 3119.279 kb on - strand at 3119.279 kb on - strand at 3119.414 kb on - strand at 3119.414 kb on - strand at 3119.516 kb on + strand at 3119.517 kb on - strand at 3119.633 kb on + strand, within RR42_RS34285 at 3119.633 kb on + strand, within RR42_RS34285 at 3119.634 kb on - strand, within RR42_RS34285 at 3119.634 kb on - strand, within RR42_RS34285 at 3119.717 kb on + strand, within RR42_RS34285 at 3119.718 kb on - strand, within RR42_RS34285 at 3119.742 kb on + strand, within RR42_RS34285 at 3119.742 kb on + strand, within RR42_RS34285 at 3119.743 kb on - strand, within RR42_RS34285 at 3119.759 kb on + strand, within RR42_RS34285 at 3119.759 kb on + strand, within RR42_RS34285 at 3119.759 kb on + strand, within RR42_RS34285 at 3119.759 kb on + strand, within RR42_RS34285 at 3119.760 kb on - strand, within RR42_RS34285 at 3119.760 kb on - strand, within RR42_RS34285 at 3119.760 kb on - strand, within RR42_RS34285 at 3119.943 kb on + strand, within RR42_RS34285 at 3119.944 kb on - strand, within RR42_RS34285
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Bacitracin 0.5 mg/ml remove 3,118,121 + -0.4 3,118,121 + +0.2 3,118,121 + -0.7 3,118,121 + -0.7 3,118,121 + +1.0 3,118,122 - -0.1 3,118,122 - -1.0 3,118,122 - -0.6 3,118,122 - +0.5 3,118,179 - +0.3 3,118,399 + +0.5 3,118,400 - -1.5 3,118,449 - +0.4 3,118,449 - -1.1 3,118,532 + -0.4 3,118,548 + -0.1 3,118,548 + -0.7 3,118,549 - +0.2 3,118,549 - +0.1 3,118,759 - RR42_RS34275 0.59 +0.7 3,118,870 + RR42_RS34275 0.86 -0.7 3,118,870 + RR42_RS34275 0.86 -0.3 3,118,870 + RR42_RS34275 0.86 -0.4 3,118,871 - RR42_RS34275 0.86 -0.5 3,119,174 + RR42_RS34280 0.81 -0.3 3,119,174 + RR42_RS34280 0.81 -0.4 3,119,175 - RR42_RS34280 0.82 -0.9 3,119,175 - RR42_RS34280 0.82 -0.6 3,119,175 - RR42_RS34280 0.82 -0.6 3,119,273 + +0.9 3,119,273 + -0.1 3,119,274 - -0.8 3,119,274 - -0.3 3,119,278 + +0.8 3,119,279 - -0.5 3,119,279 - -0.3 3,119,414 - -0.2 3,119,414 - -2.1 3,119,516 + -0.4 3,119,517 - +0.5 3,119,633 + RR42_RS34285 0.20 -0.8 3,119,633 + RR42_RS34285 0.20 -0.3 3,119,634 - RR42_RS34285 0.20 -0.2 3,119,634 - RR42_RS34285 0.20 +1.4 3,119,717 + RR42_RS34285 0.29 +0.2 3,119,718 - RR42_RS34285 0.29 -0.4 3,119,742 + RR42_RS34285 0.31 -1.4 3,119,742 + RR42_RS34285 0.31 -0.0 3,119,743 - RR42_RS34285 0.31 -1.7 3,119,759 + RR42_RS34285 0.33 +0.0 3,119,759 + RR42_RS34285 0.33 -2.5 3,119,759 + RR42_RS34285 0.33 +2.2 3,119,759 + RR42_RS34285 0.33 -0.5 3,119,760 - RR42_RS34285 0.33 -0.7 3,119,760 - RR42_RS34285 0.33 -0.3 3,119,760 - RR42_RS34285 0.33 +0.0 3,119,943 + RR42_RS34285 0.52 -0.3 3,119,944 - RR42_RS34285 0.52 -0.3
Or see this region's nucleotide sequence