Experiment: R2A with Bacitracin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS32745 and RR42_RS32750 are separated by 46 nucleotides RR42_RS32750 and RR42_RS32755 are separated by 134 nucleotides
RR42_RS32745: RR42_RS32745 - acyl-CoA dehydrogenase, at 2,741,477 to 2,742,637
_RS32745
RR42_RS32750: RR42_RS32750 - acetyl-CoA carboxylase, at 2,742,684 to 2,744,303
_RS32750
RR42_RS32755: RR42_RS32755 - IclR family transcriptional regulator, at 2,744,438 to 2,745,196
_RS32755
Position (kb)
2742
2743
2744
2745 Strain fitness (log2 ratio)
-2
-1
0
1 at 2741.712 kb on + strand, within RR42_RS32745 at 2741.712 kb on + strand, within RR42_RS32745 at 2741.712 kb on + strand, within RR42_RS32745 at 2741.712 kb on + strand, within RR42_RS32745 at 2741.713 kb on - strand, within RR42_RS32745 at 2741.713 kb on - strand, within RR42_RS32745 at 2741.730 kb on + strand, within RR42_RS32745 at 2741.925 kb on + strand, within RR42_RS32745 at 2741.930 kb on - strand, within RR42_RS32745 at 2741.930 kb on - strand, within RR42_RS32745 at 2742.124 kb on + strand, within RR42_RS32745 at 2742.125 kb on - strand, within RR42_RS32745 at 2742.195 kb on + strand, within RR42_RS32745 at 2742.196 kb on - strand, within RR42_RS32745 at 2742.356 kb on - strand, within RR42_RS32745 at 2742.408 kb on + strand, within RR42_RS32745 at 2742.408 kb on + strand, within RR42_RS32745 at 2742.409 kb on - strand, within RR42_RS32745 at 2742.465 kb on + strand, within RR42_RS32745 at 2742.631 kb on - strand at 2742.631 kb on - strand at 2742.632 kb on + strand at 2742.632 kb on + strand at 2742.633 kb on - strand at 2742.633 kb on - strand at 2743.067 kb on - strand, within RR42_RS32750 at 2743.163 kb on + strand, within RR42_RS32750 at 2743.277 kb on + strand, within RR42_RS32750 at 2743.277 kb on + strand, within RR42_RS32750 at 2743.278 kb on - strand, within RR42_RS32750 at 2743.278 kb on - strand, within RR42_RS32750 at 2743.334 kb on - strand, within RR42_RS32750 at 2743.700 kb on - strand, within RR42_RS32750 at 2743.720 kb on + strand, within RR42_RS32750 at 2743.720 kb on + strand, within RR42_RS32750 at 2743.720 kb on + strand, within RR42_RS32750 at 2743.720 kb on + strand, within RR42_RS32750 at 2743.720 kb on + strand, within RR42_RS32750 at 2743.720 kb on + strand, within RR42_RS32750 at 2743.720 kb on + strand, within RR42_RS32750 at 2743.721 kb on - strand, within RR42_RS32750 at 2743.721 kb on - strand, within RR42_RS32750 at 2743.721 kb on - strand, within RR42_RS32750 at 2743.721 kb on - strand, within RR42_RS32750 at 2743.799 kb on - strand, within RR42_RS32750 at 2743.799 kb on - strand, within RR42_RS32750 at 2743.837 kb on + strand at 2743.838 kb on - strand, within RR42_RS32750 at 2743.903 kb on - strand, within RR42_RS32750 at 2744.776 kb on + strand, within RR42_RS32755 at 2744.813 kb on + strand, within RR42_RS32755 at 2744.814 kb on - strand, within RR42_RS32755 at 2744.951 kb on + strand, within RR42_RS32755 at 2744.972 kb on + strand, within RR42_RS32755 at 2744.972 kb on + strand, within RR42_RS32755 at 2744.989 kb on + strand, within RR42_RS32755 at 2745.039 kb on - strand, within RR42_RS32755 at 2745.119 kb on - strand, within RR42_RS32755 at 2745.128 kb on - strand at 2745.128 kb on - strand at 2745.214 kb on + strand at 2745.280 kb on + strand at 2745.301 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Bacitracin 0.5 mg/ml remove 2,741,712 + RR42_RS32745 0.20 +0.3 2,741,712 + RR42_RS32745 0.20 +0.0 2,741,712 + RR42_RS32745 0.20 +1.3 2,741,712 + RR42_RS32745 0.20 +0.6 2,741,713 - RR42_RS32745 0.20 -1.5 2,741,713 - RR42_RS32745 0.20 -1.1 2,741,730 + RR42_RS32745 0.22 +0.3 2,741,925 + RR42_RS32745 0.39 +0.1 2,741,930 - RR42_RS32745 0.39 +1.0 2,741,930 - RR42_RS32745 0.39 -1.3 2,742,124 + RR42_RS32745 0.56 +1.7 2,742,125 - RR42_RS32745 0.56 -0.5 2,742,195 + RR42_RS32745 0.62 +0.4 2,742,196 - RR42_RS32745 0.62 +0.5 2,742,356 - RR42_RS32745 0.76 -2.3 2,742,408 + RR42_RS32745 0.80 +0.4 2,742,408 + RR42_RS32745 0.80 +0.4 2,742,409 - RR42_RS32745 0.80 -0.4 2,742,465 + RR42_RS32745 0.85 -0.0 2,742,631 - +1.7 2,742,631 - -0.8 2,742,632 + -0.6 2,742,632 + -0.1 2,742,633 - -0.1 2,742,633 - +0.7 2,743,067 - RR42_RS32750 0.24 -0.1 2,743,163 + RR42_RS32750 0.30 -0.6 2,743,277 + RR42_RS32750 0.37 -0.0 2,743,277 + RR42_RS32750 0.37 -1.5 2,743,278 - RR42_RS32750 0.37 -0.7 2,743,278 - RR42_RS32750 0.37 -0.1 2,743,334 - RR42_RS32750 0.40 -0.2 2,743,700 - RR42_RS32750 0.63 +0.0 2,743,720 + RR42_RS32750 0.64 +0.8 2,743,720 + RR42_RS32750 0.64 -0.4 2,743,720 + RR42_RS32750 0.64 +0.1 2,743,720 + RR42_RS32750 0.64 -0.3 2,743,720 + RR42_RS32750 0.64 -2.1 2,743,720 + RR42_RS32750 0.64 -0.4 2,743,720 + RR42_RS32750 0.64 +0.1 2,743,721 - RR42_RS32750 0.64 +0.9 2,743,721 - RR42_RS32750 0.64 -1.0 2,743,721 - RR42_RS32750 0.64 -0.2 2,743,721 - RR42_RS32750 0.64 +0.6 2,743,799 - RR42_RS32750 0.69 +0.6 2,743,799 - RR42_RS32750 0.69 +0.3 2,743,837 + -0.1 2,743,838 - RR42_RS32750 0.71 -0.3 2,743,903 - RR42_RS32750 0.75 -1.1 2,744,776 + RR42_RS32755 0.45 +0.9 2,744,813 + RR42_RS32755 0.49 +0.4 2,744,814 - RR42_RS32755 0.50 -0.5 2,744,951 + RR42_RS32755 0.68 -0.0 2,744,972 + RR42_RS32755 0.70 +0.9 2,744,972 + RR42_RS32755 0.70 +0.2 2,744,989 + RR42_RS32755 0.73 +0.5 2,745,039 - RR42_RS32755 0.79 +0.2 2,745,119 - RR42_RS32755 0.90 +0.6 2,745,128 - -0.5 2,745,128 - -0.4 2,745,214 + -0.3 2,745,280 + +0.8 2,745,301 + -0.3
Or see this region's nucleotide sequence