Experiment: R2A with Bacitracin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS32705 and RR42_RS32710 are separated by 129 nucleotides RR42_RS32710 and RR42_RS32715 are separated by 162 nucleotides RR42_RS32715 and RR42_RS32720 are separated by 344 nucleotides RR42_RS32720 and RR42_RS32725 are separated by 45 nucleotides
RR42_RS32705: RR42_RS32705 - CoA-transferase, at 2,732,364 to 2,733,581
_RS32705
RR42_RS32710: RR42_RS32710 - hypothetical protein, at 2,733,711 to 2,733,995
_RS32710
RR42_RS32715: RR42_RS32715 - peroxidase, at 2,734,158 to 2,734,811
_RS32715
RR42_RS32720: RR42_RS32720 - glyoxalase, at 2,735,156 to 2,735,536
_RS32720
RR42_RS32725: RR42_RS32725 - MFS transporter, at 2,735,582 to 2,736,544
_RS32725
Position (kb)
2734
2735 Strain fitness (log2 ratio)
-2
-1
0
1 at 2733.178 kb on + strand, within RR42_RS32705 at 2733.178 kb on + strand, within RR42_RS32705 at 2733.178 kb on + strand, within RR42_RS32705 at 2733.403 kb on + strand, within RR42_RS32705 at 2733.504 kb on - strand at 2733.579 kb on + strand at 2733.579 kb on + strand at 2733.662 kb on + strand at 2733.663 kb on - strand at 2733.677 kb on + strand at 2733.678 kb on - strand at 2733.685 kb on + strand at 2733.685 kb on + strand at 2733.686 kb on - strand at 2733.686 kb on - strand at 2733.686 kb on - strand at 2733.846 kb on + strand, within RR42_RS32710 at 2733.847 kb on - strand, within RR42_RS32710 at 2734.072 kb on + strand at 2734.073 kb on - strand at 2734.084 kb on + strand at 2734.092 kb on + strand at 2734.093 kb on - strand at 2734.159 kb on + strand at 2734.216 kb on + strand at 2734.217 kb on - strand at 2734.489 kb on + strand, within RR42_RS32715 at 2734.490 kb on - strand, within RR42_RS32715 at 2734.658 kb on + strand, within RR42_RS32715 at 2734.659 kb on - strand, within RR42_RS32715 at 2734.660 kb on + strand, within RR42_RS32715 at 2734.660 kb on + strand, within RR42_RS32715 at 2734.661 kb on - strand, within RR42_RS32715 at 2734.661 kb on - strand, within RR42_RS32715 at 2734.661 kb on - strand, within RR42_RS32715 at 2734.661 kb on - strand, within RR42_RS32715 at 2734.811 kb on + strand at 2734.812 kb on - strand at 2734.812 kb on - strand at 2734.812 kb on - strand at 2734.914 kb on + strand at 2734.914 kb on + strand at 2734.914 kb on + strand at 2734.914 kb on + strand at 2734.914 kb on + strand at 2734.942 kb on + strand at 2734.942 kb on + strand at 2735.106 kb on + strand at 2735.106 kb on + strand at 2735.106 kb on + strand at 2735.106 kb on + strand at 2735.106 kb on + strand at 2735.107 kb on - strand at 2735.107 kb on - strand at 2735.107 kb on - strand at 2735.112 kb on - strand at 2735.112 kb on - strand at 2735.222 kb on + strand, within RR42_RS32720 at 2735.222 kb on + strand, within RR42_RS32720 at 2735.223 kb on - strand, within RR42_RS32720 at 2735.223 kb on - strand, within RR42_RS32720 at 2735.288 kb on + strand, within RR42_RS32720 at 2735.288 kb on + strand, within RR42_RS32720 at 2735.288 kb on + strand, within RR42_RS32720 at 2735.334 kb on + strand, within RR42_RS32720
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Bacitracin 0.5 mg/ml remove 2,733,178 + RR42_RS32705 0.67 -0.9 2,733,178 + RR42_RS32705 0.67 -0.3 2,733,178 + RR42_RS32705 0.67 +0.7 2,733,403 + RR42_RS32705 0.85 +0.5 2,733,504 - -0.2 2,733,579 + +0.9 2,733,579 + -0.1 2,733,662 + -1.0 2,733,663 - -0.1 2,733,677 + +0.1 2,733,678 - +0.4 2,733,685 + -0.2 2,733,685 + -1.9 2,733,686 - -0.8 2,733,686 - -0.8 2,733,686 - +0.2 2,733,846 + RR42_RS32710 0.47 -1.5 2,733,847 - RR42_RS32710 0.48 -1.2 2,734,072 + +0.3 2,734,073 - +1.1 2,734,084 + +0.3 2,734,092 + +0.7 2,734,093 - -0.5 2,734,159 + +0.8 2,734,216 + +0.8 2,734,217 - -0.5 2,734,489 + RR42_RS32715 0.51 +0.5 2,734,490 - RR42_RS32715 0.51 +0.3 2,734,658 + RR42_RS32715 0.76 +0.5 2,734,659 - RR42_RS32715 0.77 -1.1 2,734,660 + RR42_RS32715 0.77 -0.1 2,734,660 + RR42_RS32715 0.77 +1.0 2,734,661 - RR42_RS32715 0.77 -1.3 2,734,661 - RR42_RS32715 0.77 -0.4 2,734,661 - RR42_RS32715 0.77 -0.6 2,734,661 - RR42_RS32715 0.77 -1.2 2,734,811 + +0.2 2,734,812 - -0.2 2,734,812 - -0.1 2,734,812 - -0.5 2,734,914 + -1.3 2,734,914 + +0.4 2,734,914 + -1.0 2,734,914 + -0.2 2,734,914 + +0.2 2,734,942 + +0.5 2,734,942 + -0.6 2,735,106 + -0.8 2,735,106 + -0.3 2,735,106 + +0.3 2,735,106 + -0.5 2,735,106 + -0.5 2,735,107 - -0.5 2,735,107 - -0.2 2,735,107 - +0.0 2,735,112 - -0.1 2,735,112 - +0.3 2,735,222 + RR42_RS32720 0.17 +0.1 2,735,222 + RR42_RS32720 0.17 +0.3 2,735,223 - RR42_RS32720 0.18 -0.3 2,735,223 - RR42_RS32720 0.18 +0.0 2,735,288 + RR42_RS32720 0.35 -0.6 2,735,288 + RR42_RS32720 0.35 -1.4 2,735,288 + RR42_RS32720 0.35 -0.6 2,735,334 + RR42_RS32720 0.47 -0.5
Or see this region's nucleotide sequence