Experiment: R2A with Bacitracin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS32695 and RR42_RS32700 are separated by 174 nucleotides RR42_RS32700 and RR42_RS32705 are separated by 81 nucleotides
RR42_RS32695: RR42_RS32695 - Fis family transcriptional regulator, at 2,729,430 to 2,731,145
_RS32695
RR42_RS32700: RR42_RS32700 - alpha/beta hydrolase, at 2,731,320 to 2,732,282
_RS32700
RR42_RS32705: RR42_RS32705 - CoA-transferase, at 2,732,364 to 2,733,581
_RS32705
Position (kb)
2731
2732
2733 Strain fitness (log2 ratio)
-1
0
1 at 2730.532 kb on + strand, within RR42_RS32695 at 2730.775 kb on + strand, within RR42_RS32695 at 2730.776 kb on - strand, within RR42_RS32695 at 2730.776 kb on - strand, within RR42_RS32695 at 2730.977 kb on + strand at 2731.017 kb on - strand at 2731.194 kb on + strand at 2731.211 kb on + strand at 2731.270 kb on + strand at 2731.299 kb on + strand at 2731.300 kb on - strand at 2731.461 kb on + strand, within RR42_RS32700 at 2731.461 kb on + strand, within RR42_RS32700 at 2731.462 kb on - strand, within RR42_RS32700 at 2731.462 kb on - strand, within RR42_RS32700 at 2731.641 kb on - strand, within RR42_RS32700 at 2731.672 kb on - strand, within RR42_RS32700 at 2732.050 kb on - strand, within RR42_RS32700 at 2732.055 kb on + strand, within RR42_RS32700 at 2732.258 kb on + strand at 2732.259 kb on - strand at 2732.280 kb on + strand at 2732.393 kb on - strand at 2732.393 kb on - strand at 2732.463 kb on + strand at 2732.464 kb on - strand at 2732.464 kb on - strand at 2732.559 kb on + strand, within RR42_RS32705 at 2732.601 kb on - strand, within RR42_RS32705 at 2732.601 kb on - strand, within RR42_RS32705 at 2732.670 kb on + strand, within RR42_RS32705 at 2732.671 kb on - strand, within RR42_RS32705 at 2732.671 kb on - strand, within RR42_RS32705 at 2732.694 kb on + strand, within RR42_RS32705 at 2732.695 kb on - strand, within RR42_RS32705 at 2732.695 kb on - strand, within RR42_RS32705 at 2732.742 kb on + strand, within RR42_RS32705 at 2732.742 kb on + strand, within RR42_RS32705 at 2732.743 kb on - strand, within RR42_RS32705 at 2732.778 kb on + strand, within RR42_RS32705 at 2732.796 kb on + strand, within RR42_RS32705 at 2732.976 kb on + strand, within RR42_RS32705 at 2733.037 kb on - strand, within RR42_RS32705 at 2733.178 kb on + strand, within RR42_RS32705 at 2733.178 kb on + strand, within RR42_RS32705 at 2733.178 kb on + strand, within RR42_RS32705
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Bacitracin 0.5 mg/ml remove 2,730,532 + RR42_RS32695 0.64 +0.0 2,730,775 + RR42_RS32695 0.78 -0.1 2,730,776 - RR42_RS32695 0.78 -0.1 2,730,776 - RR42_RS32695 0.78 -0.2 2,730,977 + -0.3 2,731,017 - +0.9 2,731,194 + -0.2 2,731,211 + +0.8 2,731,270 + +0.6 2,731,299 + -0.7 2,731,300 - +0.4 2,731,461 + RR42_RS32700 0.15 +0.9 2,731,461 + RR42_RS32700 0.15 +1.7 2,731,462 - RR42_RS32700 0.15 +0.2 2,731,462 - RR42_RS32700 0.15 -0.9 2,731,641 - RR42_RS32700 0.33 -0.1 2,731,672 - RR42_RS32700 0.37 +0.5 2,732,050 - RR42_RS32700 0.76 -0.0 2,732,055 + RR42_RS32700 0.76 +0.3 2,732,258 + +1.3 2,732,259 - -0.2 2,732,280 + -0.6 2,732,393 - -0.5 2,732,393 - +0.9 2,732,463 + +0.5 2,732,464 - -0.1 2,732,464 - -0.4 2,732,559 + RR42_RS32705 0.16 -0.3 2,732,601 - RR42_RS32705 0.19 +0.2 2,732,601 - RR42_RS32705 0.19 +0.3 2,732,670 + RR42_RS32705 0.25 -1.0 2,732,671 - RR42_RS32705 0.25 -0.3 2,732,671 - RR42_RS32705 0.25 +0.1 2,732,694 + RR42_RS32705 0.27 +0.7 2,732,695 - RR42_RS32705 0.27 +0.8 2,732,695 - RR42_RS32705 0.27 -1.5 2,732,742 + RR42_RS32705 0.31 -0.3 2,732,742 + RR42_RS32705 0.31 -0.5 2,732,743 - RR42_RS32705 0.31 +0.1 2,732,778 + RR42_RS32705 0.34 -1.1 2,732,796 + RR42_RS32705 0.35 +0.2 2,732,976 + RR42_RS32705 0.50 +0.3 2,733,037 - RR42_RS32705 0.55 +0.2 2,733,178 + RR42_RS32705 0.67 -0.9 2,733,178 + RR42_RS32705 0.67 -0.3 2,733,178 + RR42_RS32705 0.67 +0.7
Or see this region's nucleotide sequence