Experiment: R2A with Bacitracin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS32685 and RR42_RS32690 are separated by 77 nucleotides RR42_RS32690 and RR42_RS32695 are separated by 409 nucleotides RR42_RS32695 and RR42_RS32700 are separated by 174 nucleotides
RR42_RS32685: RR42_RS32685 - phenol hydroxylase, at 2,727,729 to 2,728,724
_RS32685
RR42_RS32690: RR42_RS32690 - phenol hydroxylase, at 2,728,802 to 2,729,020
_RS32690
RR42_RS32695: RR42_RS32695 - Fis family transcriptional regulator, at 2,729,430 to 2,731,145
_RS32695
RR42_RS32700: RR42_RS32700 - alpha/beta hydrolase, at 2,731,320 to 2,732,282
_RS32700
Position (kb)
2729
2730
2731
2732 Strain fitness (log2 ratio)
-2
-1
0
1 at 2728.474 kb on + strand, within RR42_RS32685 at 2728.489 kb on + strand, within RR42_RS32685 at 2728.552 kb on + strand, within RR42_RS32685 at 2728.615 kb on + strand, within RR42_RS32685 at 2728.616 kb on - strand, within RR42_RS32685 at 2728.676 kb on - strand at 2728.753 kb on + strand at 2728.754 kb on - strand at 2728.754 kb on - strand at 2729.045 kb on + strand at 2729.045 kb on + strand at 2729.054 kb on + strand at 2729.055 kb on - strand at 2729.353 kb on + strand at 2729.353 kb on + strand at 2729.354 kb on - strand at 2729.394 kb on - strand at 2729.522 kb on + strand at 2729.522 kb on + strand at 2729.523 kb on - strand at 2729.600 kb on + strand at 2729.601 kb on - strand at 2729.803 kb on + strand, within RR42_RS32695 at 2729.963 kb on - strand, within RR42_RS32695 at 2729.963 kb on - strand, within RR42_RS32695 at 2730.072 kb on + strand, within RR42_RS32695 at 2730.072 kb on + strand, within RR42_RS32695 at 2730.072 kb on + strand, within RR42_RS32695 at 2730.532 kb on + strand, within RR42_RS32695 at 2730.775 kb on + strand, within RR42_RS32695 at 2730.776 kb on - strand, within RR42_RS32695 at 2730.776 kb on - strand, within RR42_RS32695 at 2730.977 kb on + strand at 2731.017 kb on - strand at 2731.194 kb on + strand at 2731.211 kb on + strand at 2731.270 kb on + strand at 2731.299 kb on + strand at 2731.300 kb on - strand at 2731.461 kb on + strand, within RR42_RS32700 at 2731.461 kb on + strand, within RR42_RS32700 at 2731.462 kb on - strand, within RR42_RS32700 at 2731.462 kb on - strand, within RR42_RS32700 at 2731.641 kb on - strand, within RR42_RS32700 at 2731.672 kb on - strand, within RR42_RS32700 at 2732.050 kb on - strand, within RR42_RS32700 at 2732.055 kb on + strand, within RR42_RS32700
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Bacitracin 0.5 mg/ml remove 2,728,474 + RR42_RS32685 0.75 -0.0 2,728,489 + RR42_RS32685 0.76 +0.1 2,728,552 + RR42_RS32685 0.83 +0.6 2,728,615 + RR42_RS32685 0.89 -2.2 2,728,616 - RR42_RS32685 0.89 +0.3 2,728,676 - +0.3 2,728,753 + +0.1 2,728,754 - +0.1 2,728,754 - +0.4 2,729,045 + -1.0 2,729,045 + +1.2 2,729,054 + -1.1 2,729,055 - -0.3 2,729,353 + -0.1 2,729,353 + -1.8 2,729,354 - -2.1 2,729,394 - +1.2 2,729,522 + -0.2 2,729,522 + +1.1 2,729,523 - -0.1 2,729,600 + +0.7 2,729,601 - +0.1 2,729,803 + RR42_RS32695 0.22 -0.9 2,729,963 - RR42_RS32695 0.31 -1.0 2,729,963 - RR42_RS32695 0.31 -0.1 2,730,072 + RR42_RS32695 0.37 +0.3 2,730,072 + RR42_RS32695 0.37 -0.3 2,730,072 + RR42_RS32695 0.37 +0.2 2,730,532 + RR42_RS32695 0.64 +0.0 2,730,775 + RR42_RS32695 0.78 -0.1 2,730,776 - RR42_RS32695 0.78 -0.1 2,730,776 - RR42_RS32695 0.78 -0.2 2,730,977 + -0.3 2,731,017 - +0.9 2,731,194 + -0.2 2,731,211 + +0.8 2,731,270 + +0.6 2,731,299 + -0.7 2,731,300 - +0.4 2,731,461 + RR42_RS32700 0.15 +0.9 2,731,461 + RR42_RS32700 0.15 +1.7 2,731,462 - RR42_RS32700 0.15 +0.2 2,731,462 - RR42_RS32700 0.15 -0.9 2,731,641 - RR42_RS32700 0.33 -0.1 2,731,672 - RR42_RS32700 0.37 +0.5 2,732,050 - RR42_RS32700 0.76 -0.0 2,732,055 + RR42_RS32700 0.76 +0.3
Or see this region's nucleotide sequence