Experiment: R2A with Bacitracin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS31905 and RR42_RS31910 are separated by 11 nucleotides RR42_RS31910 and RR42_RS31915 are separated by 14 nucleotides RR42_RS31915 and RR42_RS31920 are separated by 50 nucleotides
RR42_RS31905: RR42_RS31905 - acyl-CoA dehydrogenase, at 2,535,045 to 2,536,271
_RS31905
RR42_RS31910: RR42_RS31910 - acyl-CoA dehydrogenase, at 2,536,283 to 2,537,527
_RS31910
RR42_RS31915: RR42_RS31915 - short-chain dehydrogenase, at 2,537,542 to 2,538,417
_RS31915
RR42_RS31920: RR42_RS31920 - acetyl-CoA acetyltransferase, at 2,538,468 to 2,539,619
_RS31920
Position (kb)
2536
2537
2538 Strain fitness (log2 ratio)
-1
0
1 at 2535.367 kb on + strand, within RR42_RS31905 at 2535.368 kb on - strand, within RR42_RS31905 at 2535.981 kb on - strand, within RR42_RS31905 at 2536.284 kb on + strand at 2536.411 kb on - strand, within RR42_RS31910 at 2536.420 kb on + strand, within RR42_RS31910 at 2536.712 kb on - strand, within RR42_RS31910 at 2537.553 kb on - strand at 2537.553 kb on - strand at 2537.771 kb on + strand, within RR42_RS31915 at 2537.771 kb on + strand, within RR42_RS31915 at 2537.772 kb on - strand, within RR42_RS31915 at 2537.774 kb on + strand, within RR42_RS31915 at 2537.936 kb on + strand, within RR42_RS31915 at 2537.936 kb on + strand, within RR42_RS31915
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Bacitracin 0.5 mg/ml remove 2,535,367 + RR42_RS31905 0.26 -0.3 2,535,368 - RR42_RS31905 0.26 -0.3 2,535,981 - RR42_RS31905 0.76 -0.1 2,536,284 + -1.2 2,536,411 - RR42_RS31910 0.10 +1.4 2,536,420 + RR42_RS31910 0.11 +1.7 2,536,712 - RR42_RS31910 0.34 -0.5 2,537,553 - +0.0 2,537,553 - -1.1 2,537,771 + RR42_RS31915 0.26 -0.3 2,537,771 + RR42_RS31915 0.26 +0.8 2,537,772 - RR42_RS31915 0.26 +1.0 2,537,774 + RR42_RS31915 0.26 +1.3 2,537,936 + RR42_RS31915 0.45 +0.1 2,537,936 + RR42_RS31915 0.45 +0.5
Or see this region's nucleotide sequence