Experiment: R2A with Bacitracin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS29870 and RR42_RS29875 are separated by 127 nucleotides RR42_RS29875 and RR42_RS29880 are separated by 10 nucleotides
RR42_RS29870: RR42_RS29870 - anhydrase, at 2,066,808 to 2,067,332
_RS29870
RR42_RS29875: RR42_RS29875 - glycolate oxidase, at 2,067,460 to 2,068,719
_RS29875
RR42_RS29880: RR42_RS29880 - glycolate oxidase, at 2,068,730 to 2,069,803
_RS29880
Position (kb)
2067
2068
2069 Strain fitness (log2 ratio)
-2
-1
0
1 at 2066.462 kb on + strand at 2066.489 kb on + strand at 2066.490 kb on - strand at 2066.537 kb on - strand at 2066.539 kb on - strand at 2066.570 kb on - strand at 2066.665 kb on + strand at 2066.809 kb on + strand at 2066.810 kb on - strand at 2066.810 kb on - strand at 2066.836 kb on + strand at 2066.836 kb on + strand at 2066.836 kb on + strand at 2066.852 kb on + strand at 2066.852 kb on + strand at 2066.853 kb on - strand at 2066.853 kb on - strand at 2066.853 kb on - strand at 2066.853 kb on - strand at 2066.853 kb on - strand at 2066.857 kb on + strand at 2066.858 kb on - strand at 2066.858 kb on - strand at 2066.902 kb on - strand, within RR42_RS29870 at 2066.902 kb on - strand, within RR42_RS29870 at 2066.902 kb on - strand, within RR42_RS29870 at 2066.910 kb on + strand, within RR42_RS29870 at 2067.050 kb on + strand, within RR42_RS29870 at 2067.051 kb on - strand, within RR42_RS29870 at 2067.051 kb on - strand, within RR42_RS29870 at 2067.051 kb on - strand, within RR42_RS29870 at 2067.111 kb on + strand, within RR42_RS29870 at 2067.112 kb on - strand, within RR42_RS29870 at 2067.276 kb on + strand, within RR42_RS29870 at 2067.277 kb on - strand, within RR42_RS29870 at 2067.280 kb on + strand at 2067.280 kb on + strand at 2067.280 kb on + strand at 2067.281 kb on - strand at 2067.281 kb on - strand at 2067.281 kb on - strand at 2067.281 kb on - strand at 2067.281 kb on - strand at 2067.323 kb on - strand at 2067.346 kb on + strand at 2067.347 kb on - strand at 2067.401 kb on + strand at 2067.401 kb on + strand at 2067.402 kb on - strand at 2067.461 kb on + strand at 2067.461 kb on + strand at 2067.465 kb on + strand at 2067.465 kb on + strand at 2067.465 kb on + strand at 2067.466 kb on - strand at 2067.469 kb on + strand at 2067.470 kb on - strand at 2067.514 kb on + strand at 2067.515 kb on - strand at 2067.515 kb on - strand at 2067.532 kb on + strand at 2067.533 kb on - strand at 2067.533 kb on - strand at 2067.672 kb on - strand, within RR42_RS29875 at 2067.672 kb on - strand, within RR42_RS29875 at 2067.672 kb on - strand, within RR42_RS29875 at 2067.937 kb on + strand, within RR42_RS29875 at 2067.937 kb on + strand, within RR42_RS29875 at 2067.937 kb on + strand, within RR42_RS29875 at 2067.937 kb on + strand, within RR42_RS29875 at 2067.937 kb on + strand, within RR42_RS29875 at 2067.938 kb on - strand, within RR42_RS29875 at 2067.938 kb on - strand, within RR42_RS29875 at 2067.938 kb on - strand, within RR42_RS29875 at 2067.938 kb on - strand, within RR42_RS29875 at 2067.951 kb on - strand, within RR42_RS29875 at 2068.050 kb on + strand, within RR42_RS29875 at 2068.051 kb on - strand, within RR42_RS29875 at 2068.051 kb on - strand, within RR42_RS29875 at 2068.123 kb on + strand, within RR42_RS29875 at 2068.269 kb on + strand, within RR42_RS29875 at 2068.270 kb on - strand, within RR42_RS29875 at 2068.298 kb on - strand, within RR42_RS29875 at 2068.351 kb on + strand, within RR42_RS29875 at 2068.352 kb on - strand, within RR42_RS29875 at 2068.352 kb on - strand, within RR42_RS29875 at 2068.352 kb on - strand, within RR42_RS29875 at 2068.352 kb on - strand, within RR42_RS29875 at 2068.438 kb on + strand, within RR42_RS29875 at 2068.438 kb on + strand, within RR42_RS29875 at 2068.438 kb on + strand, within RR42_RS29875 at 2068.439 kb on - strand, within RR42_RS29875 at 2068.439 kb on - strand, within RR42_RS29875 at 2068.439 kb on - strand, within RR42_RS29875 at 2068.449 kb on - strand, within RR42_RS29875 at 2068.562 kb on + strand, within RR42_RS29875 at 2068.562 kb on + strand, within RR42_RS29875 at 2068.562 kb on + strand, within RR42_RS29875 at 2068.563 kb on - strand, within RR42_RS29875 at 2068.563 kb on - strand, within RR42_RS29875 at 2068.584 kb on + strand, within RR42_RS29875 at 2068.584 kb on + strand, within RR42_RS29875 at 2068.608 kb on - strand at 2068.608 kb on - strand at 2068.608 kb on - strand at 2068.719 kb on + strand at 2068.720 kb on - strand at 2068.720 kb on - strand at 2068.720 kb on - strand at 2068.720 kb on - strand at 2068.720 kb on - strand at 2068.731 kb on + strand at 2068.732 kb on - strand at 2068.847 kb on - strand, within RR42_RS29880 at 2068.929 kb on + strand, within RR42_RS29880 at 2068.929 kb on + strand, within RR42_RS29880 at 2068.930 kb on - strand, within RR42_RS29880 at 2068.930 kb on - strand, within RR42_RS29880 at 2068.930 kb on - strand, within RR42_RS29880 at 2068.930 kb on - strand, within RR42_RS29880 at 2068.930 kb on - strand, within RR42_RS29880 at 2068.930 kb on - strand, within RR42_RS29880 at 2068.930 kb on - strand, within RR42_RS29880 at 2068.931 kb on + strand, within RR42_RS29880 at 2068.932 kb on - strand, within RR42_RS29880 at 2068.932 kb on - strand, within RR42_RS29880 at 2068.960 kb on + strand, within RR42_RS29880 at 2068.961 kb on - strand, within RR42_RS29880 at 2069.226 kb on - strand, within RR42_RS29880 at 2069.226 kb on - strand, within RR42_RS29880 at 2069.317 kb on + strand, within RR42_RS29880 at 2069.317 kb on + strand, within RR42_RS29880 at 2069.318 kb on - strand, within RR42_RS29880 at 2069.327 kb on + strand, within RR42_RS29880 at 2069.327 kb on + strand, within RR42_RS29880 at 2069.516 kb on - strand, within RR42_RS29880 at 2069.516 kb on - strand, within RR42_RS29880 at 2069.554 kb on - strand, within RR42_RS29880 at 2069.637 kb on + strand, within RR42_RS29880 at 2069.637 kb on + strand, within RR42_RS29880 at 2069.637 kb on + strand, within RR42_RS29880 at 2069.637 kb on + strand, within RR42_RS29880 at 2069.637 kb on + strand, within RR42_RS29880 at 2069.637 kb on + strand, within RR42_RS29880 at 2069.638 kb on - strand, within RR42_RS29880 at 2069.638 kb on - strand, within RR42_RS29880 at 2069.638 kb on - strand, within RR42_RS29880
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Bacitracin 0.5 mg/ml remove 2,066,462 + -0.2 2,066,489 + -0.2 2,066,490 - -0.5 2,066,537 - +0.9 2,066,539 - -1.0 2,066,570 - +0.9 2,066,665 + -0.3 2,066,809 + -0.8 2,066,810 - +0.4 2,066,810 - -1.0 2,066,836 + +0.4 2,066,836 + +0.1 2,066,836 + -0.9 2,066,852 + -0.4 2,066,852 + +0.2 2,066,853 - -1.7 2,066,853 - +1.7 2,066,853 - -0.9 2,066,853 - +0.4 2,066,853 - -0.4 2,066,857 + -0.1 2,066,858 - +0.2 2,066,858 - -0.2 2,066,902 - RR42_RS29870 0.18 +0.7 2,066,902 - RR42_RS29870 0.18 -0.4 2,066,902 - RR42_RS29870 0.18 +0.7 2,066,910 + RR42_RS29870 0.19 +0.6 2,067,050 + RR42_RS29870 0.46 -0.2 2,067,051 - RR42_RS29870 0.46 +0.0 2,067,051 - RR42_RS29870 0.46 -1.4 2,067,051 - RR42_RS29870 0.46 -1.0 2,067,111 + RR42_RS29870 0.58 -1.2 2,067,112 - RR42_RS29870 0.58 +1.3 2,067,276 + RR42_RS29870 0.89 -1.7 2,067,277 - RR42_RS29870 0.89 -1.0 2,067,280 + +0.1 2,067,280 + -0.1 2,067,280 + +0.3 2,067,281 - -0.1 2,067,281 - +0.4 2,067,281 - +0.5 2,067,281 - +0.2 2,067,281 - +0.7 2,067,323 - +0.3 2,067,346 + +0.0 2,067,347 - -0.4 2,067,401 + -1.1 2,067,401 + -0.4 2,067,402 - +0.2 2,067,461 + +0.5 2,067,461 + -0.5 2,067,465 + -1.4 2,067,465 + +0.3 2,067,465 + -0.2 2,067,466 - -0.7 2,067,469 + -0.2 2,067,470 - -1.0 2,067,514 + +0.0 2,067,515 - +1.4 2,067,515 - +0.6 2,067,532 + +0.1 2,067,533 - -0.6 2,067,533 - -0.2 2,067,672 - RR42_RS29875 0.17 +0.9 2,067,672 - RR42_RS29875 0.17 -0.6 2,067,672 - RR42_RS29875 0.17 -0.1 2,067,937 + RR42_RS29875 0.38 +0.0 2,067,937 + RR42_RS29875 0.38 -0.2 2,067,937 + RR42_RS29875 0.38 +0.2 2,067,937 + RR42_RS29875 0.38 +0.4 2,067,937 + RR42_RS29875 0.38 +0.5 2,067,938 - RR42_RS29875 0.38 +0.2 2,067,938 - RR42_RS29875 0.38 -0.1 2,067,938 - RR42_RS29875 0.38 -1.1 2,067,938 - RR42_RS29875 0.38 +0.5 2,067,951 - RR42_RS29875 0.39 +0.0 2,068,050 + RR42_RS29875 0.47 -1.3 2,068,051 - RR42_RS29875 0.47 -2.1 2,068,051 - RR42_RS29875 0.47 -0.9 2,068,123 + RR42_RS29875 0.53 -0.1 2,068,269 + RR42_RS29875 0.64 -0.4 2,068,270 - RR42_RS29875 0.64 +0.5 2,068,298 - RR42_RS29875 0.67 -0.6 2,068,351 + RR42_RS29875 0.71 -0.4 2,068,352 - RR42_RS29875 0.71 +0.0 2,068,352 - RR42_RS29875 0.71 +0.7 2,068,352 - RR42_RS29875 0.71 +0.8 2,068,352 - RR42_RS29875 0.71 -1.4 2,068,438 + RR42_RS29875 0.78 +1.4 2,068,438 + RR42_RS29875 0.78 -1.1 2,068,438 + RR42_RS29875 0.78 -0.0 2,068,439 - RR42_RS29875 0.78 -0.4 2,068,439 - RR42_RS29875 0.78 -1.0 2,068,439 - RR42_RS29875 0.78 +0.7 2,068,449 - RR42_RS29875 0.78 -0.1 2,068,562 + RR42_RS29875 0.87 +0.1 2,068,562 + RR42_RS29875 0.87 +0.4 2,068,562 + RR42_RS29875 0.87 +1.2 2,068,563 - RR42_RS29875 0.88 +1.3 2,068,563 - RR42_RS29875 0.88 +0.1 2,068,584 + RR42_RS29875 0.89 -0.2 2,068,584 + RR42_RS29875 0.89 -0.3 2,068,608 - -0.0 2,068,608 - +0.6 2,068,608 - +0.4 2,068,719 + +0.0 2,068,720 - +0.4 2,068,720 - +0.1 2,068,720 - +0.5 2,068,720 - -0.0 2,068,720 - +0.0 2,068,731 + +1.2 2,068,732 - -1.4 2,068,847 - RR42_RS29880 0.11 +0.1 2,068,929 + RR42_RS29880 0.19 -0.4 2,068,929 + RR42_RS29880 0.19 -0.8 2,068,930 - RR42_RS29880 0.19 -0.3 2,068,930 - RR42_RS29880 0.19 -0.1 2,068,930 - RR42_RS29880 0.19 +0.3 2,068,930 - RR42_RS29880 0.19 -0.6 2,068,930 - RR42_RS29880 0.19 +0.7 2,068,930 - RR42_RS29880 0.19 +0.1 2,068,930 - RR42_RS29880 0.19 +0.6 2,068,931 + RR42_RS29880 0.19 -0.3 2,068,932 - RR42_RS29880 0.19 -0.6 2,068,932 - RR42_RS29880 0.19 -0.0 2,068,960 + RR42_RS29880 0.21 -0.8 2,068,961 - RR42_RS29880 0.22 +0.4 2,069,226 - RR42_RS29880 0.46 +0.2 2,069,226 - RR42_RS29880 0.46 -0.8 2,069,317 + RR42_RS29880 0.55 -0.2 2,069,317 + RR42_RS29880 0.55 -0.6 2,069,318 - RR42_RS29880 0.55 -0.3 2,069,327 + RR42_RS29880 0.56 +0.6 2,069,327 + RR42_RS29880 0.56 -0.5 2,069,516 - RR42_RS29880 0.73 +0.1 2,069,516 - RR42_RS29880 0.73 -1.8 2,069,554 - RR42_RS29880 0.77 -0.4 2,069,637 + RR42_RS29880 0.84 -1.0 2,069,637 + RR42_RS29880 0.84 +0.7 2,069,637 + RR42_RS29880 0.84 -0.1 2,069,637 + RR42_RS29880 0.84 +0.3 2,069,637 + RR42_RS29880 0.84 -0.7 2,069,637 + RR42_RS29880 0.84 -0.7 2,069,638 - RR42_RS29880 0.85 +0.6 2,069,638 - RR42_RS29880 0.85 +0.1 2,069,638 - RR42_RS29880 0.85 +0.1
Or see this region's nucleotide sequence