Experiment: R2A with Bacitracin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS29240 and RR42_RS29245 overlap by 4 nucleotides RR42_RS29245 and RR42_RS29250 overlap by 8 nucleotides RR42_RS29250 and RR42_RS29255 are separated by 174 nucleotides
RR42_RS29240: RR42_RS29240 - nitrile hydratase subunit alpha, at 1,914,971 to 1,915,675
_RS29240
RR42_RS29245: RR42_RS29245 - nitrile hydratase, at 1,915,672 to 1,916,343
_RS29245
RR42_RS29250: RR42_RS29250 - nitrile hydratase, at 1,916,336 to 1,916,722
_RS29250
RR42_RS29255: RR42_RS29255 - GTP cyclohydrolase, at 1,916,897 to 1,917,493
_RS29255
Position (kb)
1915
1916
1917 Strain fitness (log2 ratio)
-2
-1
0
1 at 1914.675 kb on + strand at 1914.675 kb on + strand at 1914.675 kb on + strand at 1914.676 kb on - strand at 1914.676 kb on - strand at 1914.676 kb on - strand at 1914.763 kb on + strand at 1914.763 kb on + strand at 1914.764 kb on - strand at 1914.764 kb on - strand at 1914.764 kb on - strand at 1914.912 kb on - strand at 1915.130 kb on + strand, within RR42_RS29240 at 1915.169 kb on + strand, within RR42_RS29240 at 1915.169 kb on + strand, within RR42_RS29240 at 1915.169 kb on + strand, within RR42_RS29240 at 1915.169 kb on + strand, within RR42_RS29240 at 1915.169 kb on + strand, within RR42_RS29240 at 1915.170 kb on - strand, within RR42_RS29240 at 1915.170 kb on - strand, within RR42_RS29240 at 1915.170 kb on - strand, within RR42_RS29240 at 1915.235 kb on + strand, within RR42_RS29240 at 1915.235 kb on + strand, within RR42_RS29240 at 1915.235 kb on + strand, within RR42_RS29240 at 1915.236 kb on - strand, within RR42_RS29240 at 1915.236 kb on - strand, within RR42_RS29240 at 1915.236 kb on - strand, within RR42_RS29240 at 1915.374 kb on - strand, within RR42_RS29240 at 1915.374 kb on - strand, within RR42_RS29240 at 1915.409 kb on + strand, within RR42_RS29240 at 1915.409 kb on + strand, within RR42_RS29240 at 1915.410 kb on - strand, within RR42_RS29240 at 1915.410 kb on - strand, within RR42_RS29240 at 1915.532 kb on + strand, within RR42_RS29240 at 1915.532 kb on + strand, within RR42_RS29240 at 1915.533 kb on - strand, within RR42_RS29240 at 1915.533 kb on - strand, within RR42_RS29240 at 1915.533 kb on - strand, within RR42_RS29240 at 1915.596 kb on + strand, within RR42_RS29240 at 1915.596 kb on + strand, within RR42_RS29240 at 1915.597 kb on - strand, within RR42_RS29240 at 1915.742 kb on + strand, within RR42_RS29245 at 1915.812 kb on + strand, within RR42_RS29245 at 1915.858 kb on + strand, within RR42_RS29245 at 1915.858 kb on + strand, within RR42_RS29245 at 1915.858 kb on + strand, within RR42_RS29245 at 1915.859 kb on - strand, within RR42_RS29245 at 1915.859 kb on - strand, within RR42_RS29245 at 1915.859 kb on - strand, within RR42_RS29245 at 1915.859 kb on - strand, within RR42_RS29245 at 1915.873 kb on + strand, within RR42_RS29245 at 1915.876 kb on + strand, within RR42_RS29245 at 1915.877 kb on - strand, within RR42_RS29245 at 1916.131 kb on + strand, within RR42_RS29245 at 1916.131 kb on + strand, within RR42_RS29245 at 1916.132 kb on - strand, within RR42_RS29245 at 1916.230 kb on + strand at 1916.230 kb on + strand, within RR42_RS29245 at 1916.231 kb on - strand, within RR42_RS29245 at 1916.311 kb on + strand at 1916.312 kb on - strand at 1916.312 kb on - strand at 1916.312 kb on - strand at 1916.579 kb on + strand, within RR42_RS29250 at 1916.579 kb on + strand, within RR42_RS29250 at 1916.579 kb on + strand, within RR42_RS29250 at 1916.579 kb on + strand, within RR42_RS29250 at 1916.580 kb on - strand, within RR42_RS29250 at 1916.580 kb on - strand, within RR42_RS29250 at 1916.580 kb on - strand, within RR42_RS29250 at 1916.582 kb on + strand, within RR42_RS29250 at 1916.583 kb on - strand, within RR42_RS29250 at 1916.583 kb on - strand, within RR42_RS29250 at 1916.744 kb on + strand at 1916.825 kb on - strand at 1916.837 kb on + strand at 1916.845 kb on - strand at 1916.960 kb on - strand, within RR42_RS29255 at 1916.960 kb on - strand, within RR42_RS29255 at 1917.164 kb on + strand, within RR42_RS29255 at 1917.164 kb on + strand, within RR42_RS29255 at 1917.218 kb on + strand, within RR42_RS29255 at 1917.219 kb on - strand, within RR42_RS29255 at 1917.219 kb on - strand, within RR42_RS29255 at 1917.245 kb on - strand, within RR42_RS29255 at 1917.293 kb on + strand, within RR42_RS29255 at 1917.294 kb on - strand, within RR42_RS29255 at 1917.294 kb on - strand, within RR42_RS29255
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Bacitracin 0.5 mg/ml remove 1,914,675 + +0.9 1,914,675 + -0.4 1,914,675 + +0.1 1,914,676 - -0.3 1,914,676 - -0.8 1,914,676 - +0.4 1,914,763 + -1.3 1,914,763 + -0.1 1,914,764 - -1.4 1,914,764 - -0.1 1,914,764 - -0.4 1,914,912 - +0.4 1,915,130 + RR42_RS29240 0.23 -1.3 1,915,169 + RR42_RS29240 0.28 +0.0 1,915,169 + RR42_RS29240 0.28 +0.4 1,915,169 + RR42_RS29240 0.28 -0.1 1,915,169 + RR42_RS29240 0.28 +0.7 1,915,169 + RR42_RS29240 0.28 -0.7 1,915,170 - RR42_RS29240 0.28 -0.2 1,915,170 - RR42_RS29240 0.28 -0.3 1,915,170 - RR42_RS29240 0.28 +0.0 1,915,235 + RR42_RS29240 0.37 -0.4 1,915,235 + RR42_RS29240 0.37 -1.5 1,915,235 + RR42_RS29240 0.37 -0.0 1,915,236 - RR42_RS29240 0.38 -0.4 1,915,236 - RR42_RS29240 0.38 -0.3 1,915,236 - RR42_RS29240 0.38 +0.6 1,915,374 - RR42_RS29240 0.57 -0.3 1,915,374 - RR42_RS29240 0.57 +0.1 1,915,409 + RR42_RS29240 0.62 -0.2 1,915,409 + RR42_RS29240 0.62 +0.5 1,915,410 - RR42_RS29240 0.62 +0.9 1,915,410 - RR42_RS29240 0.62 +0.7 1,915,532 + RR42_RS29240 0.80 -0.2 1,915,532 + RR42_RS29240 0.80 -0.4 1,915,533 - RR42_RS29240 0.80 -0.9 1,915,533 - RR42_RS29240 0.80 +0.6 1,915,533 - RR42_RS29240 0.80 -0.0 1,915,596 + RR42_RS29240 0.89 +0.2 1,915,596 + RR42_RS29240 0.89 -2.4 1,915,597 - RR42_RS29240 0.89 -0.3 1,915,742 + RR42_RS29245 0.10 +0.1 1,915,812 + RR42_RS29245 0.21 -1.0 1,915,858 + RR42_RS29245 0.28 -0.7 1,915,858 + RR42_RS29245 0.28 -0.2 1,915,858 + RR42_RS29245 0.28 -0.6 1,915,859 - RR42_RS29245 0.28 +0.6 1,915,859 - RR42_RS29245 0.28 +0.1 1,915,859 - RR42_RS29245 0.28 +0.4 1,915,859 - RR42_RS29245 0.28 +0.1 1,915,873 + RR42_RS29245 0.30 -0.6 1,915,876 + RR42_RS29245 0.30 -0.4 1,915,877 - RR42_RS29245 0.31 -0.0 1,916,131 + RR42_RS29245 0.68 +0.5 1,916,131 + RR42_RS29245 0.68 +0.2 1,916,132 - RR42_RS29245 0.68 +0.5 1,916,230 + +0.6 1,916,230 + RR42_RS29245 0.83 -0.2 1,916,231 - RR42_RS29245 0.83 -0.5 1,916,311 + -1.0 1,916,312 - -0.3 1,916,312 - -0.9 1,916,312 - -0.4 1,916,579 + RR42_RS29250 0.63 -1.4 1,916,579 + RR42_RS29250 0.63 +0.1 1,916,579 + RR42_RS29250 0.63 -0.5 1,916,579 + RR42_RS29250 0.63 +0.5 1,916,580 - RR42_RS29250 0.63 -0.3 1,916,580 - RR42_RS29250 0.63 -0.4 1,916,580 - RR42_RS29250 0.63 -0.3 1,916,582 + RR42_RS29250 0.64 -0.3 1,916,583 - RR42_RS29250 0.64 -1.8 1,916,583 - RR42_RS29250 0.64 +0.3 1,916,744 + +0.1 1,916,825 - +0.7 1,916,837 + +0.1 1,916,845 - -0.3 1,916,960 - RR42_RS29255 0.11 -1.4 1,916,960 - RR42_RS29255 0.11 -0.6 1,917,164 + RR42_RS29255 0.45 -0.7 1,917,164 + RR42_RS29255 0.45 -0.3 1,917,218 + RR42_RS29255 0.54 +1.3 1,917,219 - RR42_RS29255 0.54 -0.4 1,917,219 - RR42_RS29255 0.54 -1.7 1,917,245 - RR42_RS29255 0.58 -1.6 1,917,293 + RR42_RS29255 0.66 -0.1 1,917,294 - RR42_RS29255 0.66 -0.7 1,917,294 - RR42_RS29255 0.66 -0.4
Or see this region's nucleotide sequence