Experiment: R2A with Bacitracin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS28295 and RR42_RS28300 overlap by 4 nucleotides RR42_RS28300 and RR42_RS28305 are separated by 474 nucleotides
RR42_RS28295: RR42_RS28295 - 2-amino-3-ketobutyrate coenzyme A ligase (EC 2.3.1.29) (from data) , at 1,708,250 to 1,709,452
_RS28295
RR42_RS28300: RR42_RS28300 - L-threonine 3-dehydrogenase (EC 1.1.1.103) (from data) , at 1,709,449 to 1,710,423
_RS28300
RR42_RS28305: RR42_RS28305 - L-threonine:H+ symporter (from data) , at 1,710,898 to 1,712,316
_RS28305
Position (kb)
1709
1710
1711 Strain fitness (log2 ratio)
-2
-1
0
1 at 1708.498 kb on + strand, within RR42_RS28295 at 1708.499 kb on - strand, within RR42_RS28295 at 1708.499 kb on - strand, within RR42_RS28295 at 1708.508 kb on - strand, within RR42_RS28295 at 1708.693 kb on + strand, within RR42_RS28295 at 1708.693 kb on + strand, within RR42_RS28295 at 1709.014 kb on + strand, within RR42_RS28295 at 1709.140 kb on + strand, within RR42_RS28295 at 1709.177 kb on + strand, within RR42_RS28295 at 1709.252 kb on + strand, within RR42_RS28295 at 1709.306 kb on + strand, within RR42_RS28295 at 1709.306 kb on + strand, within RR42_RS28295 at 1709.306 kb on + strand, within RR42_RS28295 at 1709.307 kb on - strand, within RR42_RS28295 at 1709.307 kb on - strand, within RR42_RS28295 at 1709.307 kb on - strand, within RR42_RS28295 at 1709.324 kb on + strand, within RR42_RS28295 at 1709.469 kb on - strand at 1709.530 kb on + strand at 1709.665 kb on + strand, within RR42_RS28300 at 1709.666 kb on - strand, within RR42_RS28300 at 1709.786 kb on + strand, within RR42_RS28300 at 1709.787 kb on - strand, within RR42_RS28300 at 1709.787 kb on - strand, within RR42_RS28300 at 1709.787 kb on - strand, within RR42_RS28300 at 1709.787 kb on - strand, within RR42_RS28300 at 1710.049 kb on + strand, within RR42_RS28300 at 1710.049 kb on + strand, within RR42_RS28300 at 1710.050 kb on - strand, within RR42_RS28300 at 1710.050 kb on - strand, within RR42_RS28300 at 1710.050 kb on - strand, within RR42_RS28300 at 1710.091 kb on + strand, within RR42_RS28300 at 1710.091 kb on + strand, within RR42_RS28300 at 1710.091 kb on + strand, within RR42_RS28300 at 1710.091 kb on + strand, within RR42_RS28300 at 1710.092 kb on - strand, within RR42_RS28300 at 1710.092 kb on - strand, within RR42_RS28300 at 1710.167 kb on - strand, within RR42_RS28300 at 1710.248 kb on - strand, within RR42_RS28300 at 1710.482 kb on + strand at 1710.483 kb on - strand at 1710.529 kb on - strand at 1710.530 kb on + strand at 1710.530 kb on + strand at 1710.531 kb on - strand at 1710.531 kb on - strand at 1710.553 kb on + strand at 1710.638 kb on + strand at 1710.675 kb on + strand at 1710.676 kb on - strand at 1710.676 kb on - strand at 1710.898 kb on - strand at 1710.898 kb on - strand at 1710.898 kb on - strand at 1710.909 kb on - strand at 1711.075 kb on + strand, within RR42_RS28305 at 1711.075 kb on + strand, within RR42_RS28305 at 1711.136 kb on - strand, within RR42_RS28305 at 1711.165 kb on + strand, within RR42_RS28305 at 1711.270 kb on + strand, within RR42_RS28305 at 1711.271 kb on - strand, within RR42_RS28305 at 1711.337 kb on - strand, within RR42_RS28305
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Bacitracin 0.5 mg/ml remove 1,708,498 + RR42_RS28295 0.21 -0.3 1,708,499 - RR42_RS28295 0.21 +0.7 1,708,499 - RR42_RS28295 0.21 -0.7 1,708,508 - RR42_RS28295 0.21 +0.2 1,708,693 + RR42_RS28295 0.37 -0.1 1,708,693 + RR42_RS28295 0.37 +0.8 1,709,014 + RR42_RS28295 0.64 -0.5 1,709,140 + RR42_RS28295 0.74 +0.7 1,709,177 + RR42_RS28295 0.77 -0.1 1,709,252 + RR42_RS28295 0.83 -2.3 1,709,306 + RR42_RS28295 0.88 -0.7 1,709,306 + RR42_RS28295 0.88 -0.0 1,709,306 + RR42_RS28295 0.88 -0.3 1,709,307 - RR42_RS28295 0.88 +1.0 1,709,307 - RR42_RS28295 0.88 +0.2 1,709,307 - RR42_RS28295 0.88 -2.7 1,709,324 + RR42_RS28295 0.89 -0.6 1,709,469 - +0.4 1,709,530 + +0.7 1,709,665 + RR42_RS28300 0.22 -0.2 1,709,666 - RR42_RS28300 0.22 +1.5 1,709,786 + RR42_RS28300 0.35 -0.9 1,709,787 - RR42_RS28300 0.35 -0.2 1,709,787 - RR42_RS28300 0.35 +0.3 1,709,787 - RR42_RS28300 0.35 +0.8 1,709,787 - RR42_RS28300 0.35 +1.1 1,710,049 + RR42_RS28300 0.62 -0.8 1,710,049 + RR42_RS28300 0.62 +0.5 1,710,050 - RR42_RS28300 0.62 +0.1 1,710,050 - RR42_RS28300 0.62 +0.7 1,710,050 - RR42_RS28300 0.62 +1.3 1,710,091 + RR42_RS28300 0.66 +0.7 1,710,091 + RR42_RS28300 0.66 +0.4 1,710,091 + RR42_RS28300 0.66 +1.1 1,710,091 + RR42_RS28300 0.66 +0.6 1,710,092 - RR42_RS28300 0.66 +0.4 1,710,092 - RR42_RS28300 0.66 +1.0 1,710,167 - RR42_RS28300 0.74 -0.6 1,710,248 - RR42_RS28300 0.82 +0.3 1,710,482 + -1.1 1,710,483 - +0.7 1,710,529 - +0.1 1,710,530 + -0.2 1,710,530 + -0.5 1,710,531 - +0.5 1,710,531 - +1.1 1,710,553 + +0.2 1,710,638 + +0.4 1,710,675 + +0.1 1,710,676 - -1.7 1,710,676 - -0.9 1,710,898 - +0.4 1,710,898 - -1.3 1,710,898 - -0.8 1,710,909 - +0.1 1,711,075 + RR42_RS28305 0.12 +0.5 1,711,075 + RR42_RS28305 0.12 +1.4 1,711,136 - RR42_RS28305 0.17 +0.2 1,711,165 + RR42_RS28305 0.19 +1.0 1,711,270 + RR42_RS28305 0.26 +1.0 1,711,271 - RR42_RS28305 0.26 -0.5 1,711,337 - RR42_RS28305 0.31 -1.9
Or see this region's nucleotide sequence