Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS28300

Experiment: R2A with Bacitracin 0.5 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS28295 and RR42_RS28300 overlap by 4 nucleotidesRR42_RS28300 and RR42_RS28305 are separated by 474 nucleotides RR42_RS28295: RR42_RS28295 - 2-amino-3-ketobutyrate coenzyme A ligase (EC 2.3.1.29) (from data), at 1,708,250 to 1,709,452 _RS28295 RR42_RS28300: RR42_RS28300 - L-threonine 3-dehydrogenase (EC 1.1.1.103) (from data), at 1,709,449 to 1,710,423 _RS28300 RR42_RS28305: RR42_RS28305 - L-threonine:H+ symporter (from data), at 1,710,898 to 1,712,316 _RS28305 Position (kb) 1709 1710 1711Strain fitness (log2 ratio) -2 -1 0 1at 1708.498 kb on + strand, within RR42_RS28295at 1708.499 kb on - strand, within RR42_RS28295at 1708.499 kb on - strand, within RR42_RS28295at 1708.508 kb on - strand, within RR42_RS28295at 1708.693 kb on + strand, within RR42_RS28295at 1708.693 kb on + strand, within RR42_RS28295at 1709.014 kb on + strand, within RR42_RS28295at 1709.140 kb on + strand, within RR42_RS28295at 1709.177 kb on + strand, within RR42_RS28295at 1709.252 kb on + strand, within RR42_RS28295at 1709.306 kb on + strand, within RR42_RS28295at 1709.306 kb on + strand, within RR42_RS28295at 1709.306 kb on + strand, within RR42_RS28295at 1709.307 kb on - strand, within RR42_RS28295at 1709.307 kb on - strand, within RR42_RS28295at 1709.307 kb on - strand, within RR42_RS28295at 1709.324 kb on + strand, within RR42_RS28295at 1709.469 kb on - strandat 1709.530 kb on + strandat 1709.665 kb on + strand, within RR42_RS28300at 1709.666 kb on - strand, within RR42_RS28300at 1709.786 kb on + strand, within RR42_RS28300at 1709.787 kb on - strand, within RR42_RS28300at 1709.787 kb on - strand, within RR42_RS28300at 1709.787 kb on - strand, within RR42_RS28300at 1709.787 kb on - strand, within RR42_RS28300at 1710.049 kb on + strand, within RR42_RS28300at 1710.049 kb on + strand, within RR42_RS28300at 1710.050 kb on - strand, within RR42_RS28300at 1710.050 kb on - strand, within RR42_RS28300at 1710.050 kb on - strand, within RR42_RS28300at 1710.091 kb on + strand, within RR42_RS28300at 1710.091 kb on + strand, within RR42_RS28300at 1710.091 kb on + strand, within RR42_RS28300at 1710.091 kb on + strand, within RR42_RS28300at 1710.092 kb on - strand, within RR42_RS28300at 1710.092 kb on - strand, within RR42_RS28300at 1710.167 kb on - strand, within RR42_RS28300at 1710.248 kb on - strand, within RR42_RS28300at 1710.482 kb on + strandat 1710.483 kb on - strandat 1710.529 kb on - strandat 1710.530 kb on + strandat 1710.530 kb on + strandat 1710.531 kb on - strandat 1710.531 kb on - strandat 1710.553 kb on + strandat 1710.638 kb on + strandat 1710.675 kb on + strandat 1710.676 kb on - strandat 1710.676 kb on - strandat 1710.898 kb on - strandat 1710.898 kb on - strandat 1710.898 kb on - strandat 1710.909 kb on - strandat 1711.075 kb on + strand, within RR42_RS28305at 1711.075 kb on + strand, within RR42_RS28305at 1711.136 kb on - strand, within RR42_RS28305at 1711.165 kb on + strand, within RR42_RS28305at 1711.270 kb on + strand, within RR42_RS28305at 1711.271 kb on - strand, within RR42_RS28305at 1711.337 kb on - strand, within RR42_RS28305

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Bacitracin 0.5 mg/ml
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1,708,498 + RR42_RS28295 0.21 -0.3
1,708,499 - RR42_RS28295 0.21 +0.7
1,708,499 - RR42_RS28295 0.21 -0.7
1,708,508 - RR42_RS28295 0.21 +0.2
1,708,693 + RR42_RS28295 0.37 -0.1
1,708,693 + RR42_RS28295 0.37 +0.8
1,709,014 + RR42_RS28295 0.64 -0.5
1,709,140 + RR42_RS28295 0.74 +0.7
1,709,177 + RR42_RS28295 0.77 -0.1
1,709,252 + RR42_RS28295 0.83 -2.3
1,709,306 + RR42_RS28295 0.88 -0.7
1,709,306 + RR42_RS28295 0.88 -0.0
1,709,306 + RR42_RS28295 0.88 -0.3
1,709,307 - RR42_RS28295 0.88 +1.0
1,709,307 - RR42_RS28295 0.88 +0.2
1,709,307 - RR42_RS28295 0.88 -2.7
1,709,324 + RR42_RS28295 0.89 -0.6
1,709,469 - +0.4
1,709,530 + +0.7
1,709,665 + RR42_RS28300 0.22 -0.2
1,709,666 - RR42_RS28300 0.22 +1.5
1,709,786 + RR42_RS28300 0.35 -0.9
1,709,787 - RR42_RS28300 0.35 -0.2
1,709,787 - RR42_RS28300 0.35 +0.3
1,709,787 - RR42_RS28300 0.35 +0.8
1,709,787 - RR42_RS28300 0.35 +1.1
1,710,049 + RR42_RS28300 0.62 -0.8
1,710,049 + RR42_RS28300 0.62 +0.5
1,710,050 - RR42_RS28300 0.62 +0.1
1,710,050 - RR42_RS28300 0.62 +0.7
1,710,050 - RR42_RS28300 0.62 +1.3
1,710,091 + RR42_RS28300 0.66 +0.7
1,710,091 + RR42_RS28300 0.66 +0.4
1,710,091 + RR42_RS28300 0.66 +1.1
1,710,091 + RR42_RS28300 0.66 +0.6
1,710,092 - RR42_RS28300 0.66 +0.4
1,710,092 - RR42_RS28300 0.66 +1.0
1,710,167 - RR42_RS28300 0.74 -0.6
1,710,248 - RR42_RS28300 0.82 +0.3
1,710,482 + -1.1
1,710,483 - +0.7
1,710,529 - +0.1
1,710,530 + -0.2
1,710,530 + -0.5
1,710,531 - +0.5
1,710,531 - +1.1
1,710,553 + +0.2
1,710,638 + +0.4
1,710,675 + +0.1
1,710,676 - -1.7
1,710,676 - -0.9
1,710,898 - +0.4
1,710,898 - -1.3
1,710,898 - -0.8
1,710,909 - +0.1
1,711,075 + RR42_RS28305 0.12 +0.5
1,711,075 + RR42_RS28305 0.12 +1.4
1,711,136 - RR42_RS28305 0.17 +0.2
1,711,165 + RR42_RS28305 0.19 +1.0
1,711,270 + RR42_RS28305 0.26 +1.0
1,711,271 - RR42_RS28305 0.26 -0.5
1,711,337 - RR42_RS28305 0.31 -1.9

Or see this region's nucleotide sequence