Experiment: R2A with Bacitracin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS23590 and RR42_RS23595 are separated by 187 nucleotides RR42_RS23595 and RR42_RS23600 overlap by 4 nucleotides RR42_RS23600 and RR42_RS23605 overlap by 4 nucleotides
RR42_RS23590: RR42_RS23590 - FkbM family methyltransferase, at 617,882 to 618,952
_RS23590
RR42_RS23595: RR42_RS23595 - dTDP-4-dehydrorhamnose 3,5-epimerase, at 619,140 to 619,685
_RS23595
RR42_RS23600: RR42_RS23600 - SAM-dependent methyltransferase, at 619,682 to 620,965
_RS23600
RR42_RS23605: RR42_RS23605 - CDP-glucose 4,6-dehydratase, at 620,962 to 622,020
_RS23605
Position (kb) 619
620
621 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 618.783 kb on + strand, within RR42_RS23590 at 618.783 kb on + strand, within RR42_RS23590 at 618.783 kb on + strand, within RR42_RS23590 at 618.784 kb on - strand, within RR42_RS23590 at 618.784 kb on - strand, within RR42_RS23590 at 619.120 kb on + strand at 619.291 kb on + strand, within RR42_RS23595 at 619.292 kb on - strand, within RR42_RS23595 at 619.292 kb on - strand, within RR42_RS23595 at 619.351 kb on + strand, within RR42_RS23595 at 619.402 kb on + strand, within RR42_RS23595 at 619.415 kb on - strand, within RR42_RS23595 at 619.798 kb on - strand at 619.896 kb on + strand, within RR42_RS23600 at 619.897 kb on - strand, within RR42_RS23600 at 619.897 kb on - strand, within RR42_RS23600 at 619.927 kb on - strand, within RR42_RS23600 at 619.939 kb on + strand, within RR42_RS23600 at 619.939 kb on + strand, within RR42_RS23600 at 619.940 kb on - strand, within RR42_RS23600 at 619.963 kb on - strand, within RR42_RS23600 at 620.250 kb on + strand, within RR42_RS23600 at 620.250 kb on + strand, within RR42_RS23600 at 620.251 kb on - strand, within RR42_RS23600 at 620.256 kb on + strand, within RR42_RS23600 at 620.568 kb on + strand, within RR42_RS23600 at 620.569 kb on - strand, within RR42_RS23600 at 620.655 kb on + strand, within RR42_RS23600 at 620.688 kb on + strand, within RR42_RS23600 at 620.700 kb on + strand, within RR42_RS23600 at 620.707 kb on - strand, within RR42_RS23600 at 620.754 kb on - strand, within RR42_RS23600 at 621.036 kb on - strand at 621.314 kb on + strand, within RR42_RS23605 at 621.555 kb on - strand, within RR42_RS23605 at 621.555 kb on - strand, within RR42_RS23605 at 621.555 kb on - strand, within RR42_RS23605 at 621.618 kb on - strand, within RR42_RS23605 at 621.911 kb on + strand, within RR42_RS23605 at 621.947 kb on - strand at 621.947 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Bacitracin 0.5 mg/ml remove 618,783 + RR42_RS23590 0.84 -0.4 618,783 + RR42_RS23590 0.84 -1.4 618,783 + RR42_RS23590 0.84 +0.2 618,784 - RR42_RS23590 0.84 +0.4 618,784 - RR42_RS23590 0.84 +0.8 619,120 + +0.6 619,291 + RR42_RS23595 0.28 -0.6 619,292 - RR42_RS23595 0.28 -0.3 619,292 - RR42_RS23595 0.28 -0.1 619,351 + RR42_RS23595 0.39 +1.0 619,402 + RR42_RS23595 0.48 +0.6 619,415 - RR42_RS23595 0.50 -0.9 619,798 - +0.6 619,896 + RR42_RS23600 0.17 +0.7 619,897 - RR42_RS23600 0.17 +0.7 619,897 - RR42_RS23600 0.17 -3.6 619,927 - RR42_RS23600 0.19 -0.0 619,939 + RR42_RS23600 0.20 -0.6 619,939 + RR42_RS23600 0.20 -1.1 619,940 - RR42_RS23600 0.20 +0.2 619,963 - RR42_RS23600 0.22 +0.2 620,250 + RR42_RS23600 0.44 +1.2 620,250 + RR42_RS23600 0.44 +0.4 620,251 - RR42_RS23600 0.44 -0.7 620,256 + RR42_RS23600 0.45 +1.2 620,568 + RR42_RS23600 0.69 +0.1 620,569 - RR42_RS23600 0.69 +0.7 620,655 + RR42_RS23600 0.76 -0.2 620,688 + RR42_RS23600 0.78 +0.1 620,700 + RR42_RS23600 0.79 +0.8 620,707 - RR42_RS23600 0.80 +0.0 620,754 - RR42_RS23600 0.83 +0.7 621,036 - -2.3 621,314 + RR42_RS23605 0.33 +0.3 621,555 - RR42_RS23605 0.56 +1.0 621,555 - RR42_RS23605 0.56 +0.7 621,555 - RR42_RS23605 0.56 -1.6 621,618 - RR42_RS23605 0.62 -0.2 621,911 + RR42_RS23605 0.90 -0.2 621,947 - +0.5 621,947 - -1.1
Or see this region's nucleotide sequence