Experiment: R2A with Bacitracin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS23445 and RR42_RS23450 are separated by 32 nucleotides RR42_RS23450 and RR42_RS23455 are separated by 3 nucleotides RR42_RS23455 and RR42_RS23460 overlap by 62 nucleotides RR42_RS23460 and RR42_RS23465 are separated by 230 nucleotides
RR42_RS23445: RR42_RS23445 - LacI family transcriptional regulator, at 586,418 to 587,410
_RS23445
RR42_RS23450: RR42_RS23450 - enoyl-CoA hydratase, at 587,443 to 588,237
_RS23450
RR42_RS23455: RR42_RS23455 - 6-carboxyhexanoate--CoA ligase, at 588,241 to 590,331
_RS23455
RR42_RS23460: RR42_RS23460 - alpha/beta hydrolase, at 590,270 to 590,644
_RS23460
RR42_RS23465: RR42_RS23465 - 2-methylcitrate dehydratase, at 590,875 to 592,209
_RS23465
Position (kb) 588
589
590
591 Strain fitness (log2 ratio) -1
0
1 at 587.345 kb on + strand at 587.346 kb on - strand at 587.350 kb on - strand at 587.409 kb on - strand at 587.453 kb on - strand at 587.474 kb on - strand at 587.646 kb on - strand, within RR42_RS23450 at 587.646 kb on - strand, within RR42_RS23450 at 587.725 kb on + strand, within RR42_RS23450 at 587.726 kb on - strand, within RR42_RS23450 at 587.745 kb on + strand, within RR42_RS23450 at 587.746 kb on - strand, within RR42_RS23450 at 587.746 kb on - strand, within RR42_RS23450 at 587.746 kb on - strand, within RR42_RS23450 at 587.746 kb on - strand, within RR42_RS23450 at 587.863 kb on + strand, within RR42_RS23450 at 587.863 kb on + strand, within RR42_RS23450 at 587.902 kb on + strand, within RR42_RS23450 at 587.902 kb on + strand, within RR42_RS23450 at 587.903 kb on - strand, within RR42_RS23450 at 587.952 kb on + strand, within RR42_RS23450 at 588.172 kb on + strand at 588.172 kb on + strand at 588.249 kb on + strand at 588.249 kb on + strand at 588.364 kb on - strand at 588.364 kb on - strand at 588.441 kb on - strand at 588.459 kb on - strand, within RR42_RS23455 at 588.606 kb on + strand, within RR42_RS23455 at 588.837 kb on + strand, within RR42_RS23455 at 588.844 kb on + strand, within RR42_RS23455 at 588.883 kb on + strand, within RR42_RS23455 at 589.167 kb on + strand, within RR42_RS23455 at 589.378 kb on + strand, within RR42_RS23455 at 589.486 kb on + strand, within RR42_RS23455 at 590.204 kb on + strand at 590.574 kb on + strand, within RR42_RS23460 at 590.575 kb on - strand, within RR42_RS23460 at 590.661 kb on + strand at 590.662 kb on - strand at 590.662 kb on - strand at 590.783 kb on + strand at 590.783 kb on + strand at 590.843 kb on - strand at 591.166 kb on + strand, within RR42_RS23465 at 591.167 kb on - strand, within RR42_RS23465 at 591.167 kb on - strand, within RR42_RS23465 at 591.167 kb on - strand, within RR42_RS23465 at 591.184 kb on - strand, within RR42_RS23465 at 591.219 kb on - strand, within RR42_RS23465 at 591.219 kb on - strand, within RR42_RS23465 at 591.273 kb on + strand, within RR42_RS23465 at 591.314 kb on - strand, within RR42_RS23465
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Bacitracin 0.5 mg/ml remove 587,345 + +1.6 587,346 - -0.3 587,350 - +0.4 587,409 - -0.9 587,453 - -0.1 587,474 - +0.2 587,646 - RR42_RS23450 0.26 -0.7 587,646 - RR42_RS23450 0.26 +1.1 587,725 + RR42_RS23450 0.35 +0.2 587,726 - RR42_RS23450 0.36 +0.2 587,745 + RR42_RS23450 0.38 -0.6 587,746 - RR42_RS23450 0.38 -0.4 587,746 - RR42_RS23450 0.38 -0.4 587,746 - RR42_RS23450 0.38 -0.5 587,746 - RR42_RS23450 0.38 +1.0 587,863 + RR42_RS23450 0.53 +0.2 587,863 + RR42_RS23450 0.53 +1.7 587,902 + RR42_RS23450 0.58 +0.8 587,902 + RR42_RS23450 0.58 +0.3 587,903 - RR42_RS23450 0.58 +0.0 587,952 + RR42_RS23450 0.64 +0.4 588,172 + +0.5 588,172 + +1.4 588,249 + +0.8 588,249 + -0.4 588,364 - +0.0 588,364 - -0.5 588,441 - +0.3 588,459 - RR42_RS23455 0.10 +0.5 588,606 + RR42_RS23455 0.17 +1.3 588,837 + RR42_RS23455 0.29 +0.3 588,844 + RR42_RS23455 0.29 +0.7 588,883 + RR42_RS23455 0.31 -0.0 589,167 + RR42_RS23455 0.44 +0.3 589,378 + RR42_RS23455 0.54 +0.9 589,486 + RR42_RS23455 0.60 +0.1 590,204 + +1.1 590,574 + RR42_RS23460 0.81 -0.5 590,575 - RR42_RS23460 0.81 -0.3 590,661 + +0.3 590,662 - -0.1 590,662 - -0.8 590,783 + +0.4 590,783 + +0.6 590,843 - +1.3 591,166 + RR42_RS23465 0.22 -0.0 591,167 - RR42_RS23465 0.22 +0.4 591,167 - RR42_RS23465 0.22 -0.0 591,167 - RR42_RS23465 0.22 -0.2 591,184 - RR42_RS23465 0.23 -0.5 591,219 - RR42_RS23465 0.26 -1.2 591,219 - RR42_RS23465 0.26 -0.1 591,273 + RR42_RS23465 0.30 -1.0 591,314 - RR42_RS23465 0.33 -0.2
Or see this region's nucleotide sequence