Experiment: R2A with Bacitracin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS22135 and RR42_RS22140 are separated by 1 nucleotides RR42_RS22140 and RR42_RS22145 overlap by 4 nucleotides RR42_RS22145 and RR42_RS22150 are separated by 37 nucleotides RR42_RS22150 and RR42_RS22155 are separated by 7 nucleotides
RR42_RS22135: RR42_RS22135 - cytochrome C, at 306,970 to 307,455
_RS22135
RR42_RS22140: RR42_RS22140 - cytochrome C, at 307,457 to 307,912
_RS22140
RR42_RS22145: RR42_RS22145 - amine dehydrogenase, at 307,909 to 308,430
_RS22145
RR42_RS22150: RR42_RS22150 - thiol-disulfide isomerase, at 308,468 to 309,055
_RS22150
RR42_RS22155: RR42_RS22155 - hypothetical protein, at 309,063 to 309,686
_RS22155
Position (kb) 307
308
309 Strain fitness (log2 ratio) -1
0
1 at 307.331 kb on + strand, within RR42_RS22135 at 307.331 kb on + strand, within RR42_RS22135 at 307.332 kb on - strand, within RR42_RS22135 at 307.458 kb on + strand at 307.458 kb on + strand at 307.459 kb on - strand at 308.079 kb on - strand, within RR42_RS22145 at 308.090 kb on + strand, within RR42_RS22145 at 308.216 kb on + strand, within RR42_RS22145 at 308.216 kb on + strand, within RR42_RS22145 at 308.217 kb on - strand, within RR42_RS22145 at 308.510 kb on - strand at 308.655 kb on + strand, within RR42_RS22150 at 308.988 kb on + strand, within RR42_RS22150 at 308.988 kb on + strand, within RR42_RS22150 at 309.031 kb on + strand at 309.084 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Bacitracin 0.5 mg/ml remove 307,331 + RR42_RS22135 0.74 -0.8 307,331 + RR42_RS22135 0.74 +0.5 307,332 - RR42_RS22135 0.74 +0.7 307,458 + -0.3 307,458 + -1.6 307,459 - +0.3 308,079 - RR42_RS22145 0.33 +0.6 308,090 + RR42_RS22145 0.35 +0.9 308,216 + RR42_RS22145 0.59 +0.5 308,216 + RR42_RS22145 0.59 +0.3 308,217 - RR42_RS22145 0.59 +0.2 308,510 - -0.2 308,655 + RR42_RS22150 0.32 +0.2 308,988 + RR42_RS22150 0.88 -0.1 308,988 + RR42_RS22150 0.88 -1.1 309,031 + -0.2 309,084 - +0.8
Or see this region's nucleotide sequence