Experiment: R2A with Bacitracin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS18845 and RR42_RS18850 are separated by 247 nucleotides RR42_RS18850 and RR42_RS18855 are separated by 130 nucleotides RR42_RS18855 and RR42_RS18860 are separated by 151 nucleotides
RR42_RS18845: RR42_RS18845 - membrane protein, at 4,086,024 to 4,087,124
_RS18845
RR42_RS18850: RR42_RS18850 - preprotein translocase subunit TatC, at 4,087,372 to 4,088,169
_RS18850
RR42_RS18855: RR42_RS18855 - preprotein translocase, at 4,088,300 to 4,088,809
_RS18855
RR42_RS18860: RR42_RS18860 - protein translocase TatA, at 4,088,961 to 4,089,197
_RS18860
Position (kb) 4087
4088
4089 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 4087.298 kb on + strand at 4087.299 kb on - strand at 4087.333 kb on - strand at 4087.455 kb on - strand, within RR42_RS18850 at 4087.548 kb on - strand, within RR42_RS18850 at 4087.548 kb on - strand, within RR42_RS18850 at 4087.670 kb on + strand, within RR42_RS18850 at 4087.758 kb on - strand, within RR42_RS18850 at 4088.219 kb on + strand at 4088.220 kb on - strand at 4088.220 kb on - strand at 4088.220 kb on - strand at 4088.381 kb on + strand, within RR42_RS18855 at 4088.381 kb on + strand, within RR42_RS18855 at 4088.382 kb on - strand, within RR42_RS18855 at 4088.400 kb on + strand, within RR42_RS18855 at 4088.683 kb on + strand, within RR42_RS18855 at 4088.683 kb on + strand, within RR42_RS18855 at 4088.684 kb on - strand, within RR42_RS18855 at 4088.684 kb on - strand, within RR42_RS18855 at 4088.963 kb on - strand at 4088.963 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Bacitracin 0.5 mg/ml remove 4,087,298 + -4.1 4,087,299 - -2.1 4,087,333 - +0.3 4,087,455 - RR42_RS18850 0.10 -2.9 4,087,548 - RR42_RS18850 0.22 -2.7 4,087,548 - RR42_RS18850 0.22 -3.4 4,087,670 + RR42_RS18850 0.37 -2.9 4,087,758 - RR42_RS18850 0.48 -0.9 4,088,219 + -2.5 4,088,220 - +0.4 4,088,220 - +0.1 4,088,220 - -0.8 4,088,381 + RR42_RS18855 0.16 -4.1 4,088,381 + RR42_RS18855 0.16 -2.2 4,088,382 - RR42_RS18855 0.16 -0.5 4,088,400 + RR42_RS18855 0.20 -1.4 4,088,683 + RR42_RS18855 0.75 -3.3 4,088,683 + RR42_RS18855 0.75 -0.1 4,088,684 - RR42_RS18855 0.75 -1.4 4,088,684 - RR42_RS18855 0.75 -1.4 4,088,963 - +0.4 4,088,963 - +2.5
Or see this region's nucleotide sequence