Experiment: R2A with Bacitracin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS16050 and RR42_RS16055 are separated by 138 nucleotides RR42_RS16055 and RR42_RS16060 are separated by 502 nucleotides
RR42_RS16050: RR42_RS16050 - segregation and condensation protein A, at 3,476,773 to 3,477,654
_RS16050
RR42_RS16055: RR42_RS16055 - pantothenate synthetase, at 3,477,793 to 3,478,641
_RS16055
RR42_RS16060: RR42_RS16060 - membrane protein, at 3,479,144 to 3,480,319
_RS16060
Position (kb)
3477
3478
3479 Strain fitness (log2 ratio)
-1
0
1 at 3477.640 kb on + strand at 3477.641 kb on - strand at 3477.694 kb on + strand at 3477.694 kb on + strand at 3477.694 kb on + strand at 3477.695 kb on - strand at 3477.721 kb on + strand at 3477.721 kb on + strand at 3477.769 kb on + strand at 3477.769 kb on + strand at 3478.683 kb on + strand at 3478.683 kb on + strand at 3478.683 kb on + strand at 3478.683 kb on + strand at 3478.684 kb on - strand at 3478.684 kb on - strand at 3478.684 kb on - strand at 3478.684 kb on - strand at 3478.684 kb on - strand at 3478.684 kb on - strand at 3478.684 kb on - strand at 3478.684 kb on - strand at 3478.851 kb on - strand at 3478.967 kb on + strand at 3479.033 kb on + strand at 3479.033 kb on + strand at 3479.257 kb on + strand at 3479.294 kb on - strand, within RR42_RS16060 at 3479.294 kb on - strand, within RR42_RS16060 at 3479.305 kb on - strand, within RR42_RS16060 at 3479.305 kb on - strand, within RR42_RS16060 at 3479.375 kb on - strand, within RR42_RS16060 at 3479.502 kb on + strand, within RR42_RS16060 at 3479.503 kb on - strand, within RR42_RS16060 at 3479.540 kb on + strand, within RR42_RS16060 at 3479.540 kb on + strand, within RR42_RS16060 at 3479.540 kb on + strand, within RR42_RS16060 at 3479.540 kb on + strand, within RR42_RS16060 at 3479.541 kb on - strand, within RR42_RS16060 at 3479.541 kb on - strand, within RR42_RS16060 at 3479.541 kb on - strand, within RR42_RS16060 at 3479.541 kb on - strand, within RR42_RS16060 at 3479.541 kb on - strand, within RR42_RS16060
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Bacitracin 0.5 mg/ml remove 3,477,640 + -0.9 3,477,641 - -0.2 3,477,694 + -1.7 3,477,694 + -0.6 3,477,694 + -0.8 3,477,695 - -1.5 3,477,721 + -0.6 3,477,721 + -1.4 3,477,769 + -0.8 3,477,769 + -0.4 3,478,683 + +0.3 3,478,683 + -0.7 3,478,683 + +0.2 3,478,683 + -0.5 3,478,684 - -0.1 3,478,684 - +0.3 3,478,684 - -0.4 3,478,684 - -0.3 3,478,684 - -1.0 3,478,684 - +0.6 3,478,684 - +0.7 3,478,684 - +0.3 3,478,851 - -1.2 3,478,967 + +1.1 3,479,033 + -1.3 3,479,033 + -0.2 3,479,257 + -1.0 3,479,294 - RR42_RS16060 0.13 +0.6 3,479,294 - RR42_RS16060 0.13 -0.6 3,479,305 - RR42_RS16060 0.14 +1.1 3,479,305 - RR42_RS16060 0.14 -0.1 3,479,375 - RR42_RS16060 0.20 -1.5 3,479,502 + RR42_RS16060 0.30 -0.8 3,479,503 - RR42_RS16060 0.31 -1.0 3,479,540 + RR42_RS16060 0.34 -0.1 3,479,540 + RR42_RS16060 0.34 -0.1 3,479,540 + RR42_RS16060 0.34 -0.3 3,479,540 + RR42_RS16060 0.34 -0.2 3,479,541 - RR42_RS16060 0.34 +1.2 3,479,541 - RR42_RS16060 0.34 -0.0 3,479,541 - RR42_RS16060 0.34 +0.3 3,479,541 - RR42_RS16060 0.34 -0.7 3,479,541 - RR42_RS16060 0.34 +0.2
Or see this region's nucleotide sequence