Experiment: R2A with Bacitracin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS15220 and RR42_RS15225 are separated by 4 nucleotides RR42_RS15225 and RR42_RS15230 are separated by 79 nucleotides RR42_RS15230 and RR42_RS15235 are separated by 11 nucleotides RR42_RS15235 and RR42_RS15240 are separated by 145 nucleotides RR42_RS15240 and RR42_RS15245 are separated by 99 nucleotides
RR42_RS15220: RR42_RS15220 - glutaminyl-tRNA synthetase, at 3,296,908 to 3,298,686
_RS15220
RR42_RS15225: RR42_RS15225 - RNA-binding protein, at 3,298,691 to 3,298,909
_RS15225
RR42_RS15230: RR42_RS15230 - membrane protein, at 3,298,989 to 3,299,372
_RS15230
RR42_RS15235: RR42_RS15235 - hypothetical protein, at 3,299,384 to 3,299,986
_RS15235
RR42_RS15240: RR42_RS15240 - membrane protein, at 3,300,132 to 3,300,443
_RS15240
RR42_RS15245: RR42_RS15245 - alpha/beta hydrolase, at 3,300,543 to 3,300,941
_RS15245
Position (kb)
3299
3300 Strain fitness (log2 ratio)
-1
0
1
2 at 3298.685 kb on - strand at 3298.685 kb on - strand at 3298.726 kb on + strand, within RR42_RS15225 at 3299.265 kb on + strand, within RR42_RS15230 at 3299.266 kb on - strand, within RR42_RS15230 at 3299.266 kb on - strand, within RR42_RS15230 at 3299.277 kb on + strand, within RR42_RS15230 at 3299.277 kb on + strand, within RR42_RS15230 at 3299.343 kb on - strand at 3299.343 kb on - strand at 3299.498 kb on + strand, within RR42_RS15235 at 3299.498 kb on + strand, within RR42_RS15235 at 3299.499 kb on - strand, within RR42_RS15235 at 3299.499 kb on - strand, within RR42_RS15235 at 3299.499 kb on - strand, within RR42_RS15235 at 3299.922 kb on + strand, within RR42_RS15235 at 3300.062 kb on - strand at 3300.252 kb on + strand, within RR42_RS15240 at 3300.252 kb on + strand, within RR42_RS15240 at 3300.253 kb on - strand, within RR42_RS15240 at 3300.253 kb on - strand, within RR42_RS15240 at 3300.253 kb on - strand, within RR42_RS15240 at 3300.253 kb on - strand, within RR42_RS15240 at 3300.253 kb on - strand, within RR42_RS15240 at 3300.412 kb on - strand at 3300.412 kb on - strand at 3300.412 kb on - strand at 3300.414 kb on + strand at 3300.426 kb on + strand at 3300.427 kb on - strand at 3300.838 kb on + strand, within RR42_RS15245 at 3300.850 kb on - strand, within RR42_RS15245 at 3300.890 kb on + strand, within RR42_RS15245
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Bacitracin 0.5 mg/ml remove 3,298,685 - -0.4 3,298,685 - +0.3 3,298,726 + RR42_RS15225 0.16 -0.5 3,299,265 + RR42_RS15230 0.72 +0.1 3,299,266 - RR42_RS15230 0.72 -0.1 3,299,266 - RR42_RS15230 0.72 +1.1 3,299,277 + RR42_RS15230 0.75 +0.2 3,299,277 + RR42_RS15230 0.75 +0.3 3,299,343 - -0.6 3,299,343 - -0.5 3,299,498 + RR42_RS15235 0.19 -0.8 3,299,498 + RR42_RS15235 0.19 +0.6 3,299,499 - RR42_RS15235 0.19 -0.3 3,299,499 - RR42_RS15235 0.19 +2.5 3,299,499 - RR42_RS15235 0.19 -0.1 3,299,922 + RR42_RS15235 0.89 -1.0 3,300,062 - -0.3 3,300,252 + RR42_RS15240 0.38 -0.7 3,300,252 + RR42_RS15240 0.38 +0.2 3,300,253 - RR42_RS15240 0.39 -1.2 3,300,253 - RR42_RS15240 0.39 -0.1 3,300,253 - RR42_RS15240 0.39 +0.1 3,300,253 - RR42_RS15240 0.39 +0.1 3,300,253 - RR42_RS15240 0.39 -0.2 3,300,412 - -0.1 3,300,412 - +0.0 3,300,412 - +0.7 3,300,414 + -0.3 3,300,426 + +0.2 3,300,427 - +0.2 3,300,838 + RR42_RS15245 0.74 -1.2 3,300,850 - RR42_RS15245 0.77 -0.7 3,300,890 + RR42_RS15245 0.87 -0.2
Or see this region's nucleotide sequence