Experiment: R2A with Bacitracin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS15150 and RR42_RS15155 are separated by 344 nucleotides RR42_RS15155 and RR42_RS15160 are separated by 203 nucleotides RR42_RS15160 and RR42_RS15165 are separated by 49 nucleotides
RR42_RS15150: RR42_RS15150 - alanyl-tRNA synthetase, at 3,278,351 to 3,280,975
_RS15150
RR42_RS15155: RR42_RS15155 - CoA-transferase, at 3,281,320 to 3,282,540
_RS15155
RR42_RS15160: RR42_RS15160 - membrane protein, at 3,282,744 to 3,283,199
_RS15160
RR42_RS15165: RR42_RS15165 - ABC transporter substrate-binding protein, at 3,283,249 to 3,284,271
_RS15165
Position (kb)
3281
3282
3283 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3281.177 kb on - strand at 3281.177 kb on - strand at 3281.330 kb on + strand at 3281.512 kb on + strand, within RR42_RS15155 at 3281.512 kb on + strand, within RR42_RS15155 at 3281.512 kb on + strand, within RR42_RS15155 at 3281.512 kb on + strand, within RR42_RS15155 at 3281.512 kb on + strand, within RR42_RS15155 at 3281.512 kb on + strand, within RR42_RS15155 at 3281.515 kb on + strand, within RR42_RS15155 at 3281.516 kb on - strand, within RR42_RS15155 at 3281.516 kb on - strand, within RR42_RS15155 at 3281.626 kb on + strand, within RR42_RS15155 at 3281.626 kb on + strand, within RR42_RS15155 at 3281.627 kb on - strand, within RR42_RS15155 at 3281.627 kb on - strand, within RR42_RS15155 at 3281.627 kb on - strand, within RR42_RS15155 at 3281.627 kb on - strand, within RR42_RS15155 at 3281.627 kb on - strand, within RR42_RS15155 at 3281.650 kb on + strand, within RR42_RS15155 at 3281.651 kb on - strand, within RR42_RS15155 at 3281.662 kb on + strand, within RR42_RS15155 at 3281.698 kb on + strand, within RR42_RS15155 at 3281.698 kb on + strand, within RR42_RS15155 at 3281.698 kb on + strand, within RR42_RS15155 at 3281.699 kb on - strand, within RR42_RS15155 at 3281.699 kb on - strand, within RR42_RS15155 at 3281.699 kb on - strand, within RR42_RS15155 at 3281.873 kb on - strand, within RR42_RS15155 at 3281.932 kb on + strand, within RR42_RS15155 at 3281.933 kb on - strand, within RR42_RS15155 at 3281.992 kb on + strand, within RR42_RS15155 at 3281.993 kb on - strand, within RR42_RS15155 at 3281.993 kb on - strand, within RR42_RS15155 at 3281.993 kb on - strand, within RR42_RS15155 at 3282.042 kb on + strand, within RR42_RS15155 at 3282.043 kb on - strand, within RR42_RS15155 at 3282.043 kb on - strand, within RR42_RS15155 at 3282.493 kb on + strand at 3282.493 kb on + strand at 3282.495 kb on + strand at 3282.777 kb on + strand at 3282.777 kb on + strand at 3282.777 kb on + strand at 3282.777 kb on + strand at 3282.778 kb on - strand at 3282.778 kb on - strand at 3282.778 kb on - strand at 3282.873 kb on + strand, within RR42_RS15160 at 3282.873 kb on + strand, within RR42_RS15160 at 3282.886 kb on - strand, within RR42_RS15160 at 3282.886 kb on - strand, within RR42_RS15160 at 3282.886 kb on - strand, within RR42_RS15160 at 3283.312 kb on - strand at 3283.312 kb on - strand at 3283.507 kb on + strand, within RR42_RS15165 at 3283.534 kb on + strand, within RR42_RS15165 at 3283.534 kb on + strand, within RR42_RS15165
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Bacitracin 0.5 mg/ml remove 3,281,177 - +1.0 3,281,177 - +1.5 3,281,330 + -0.2 3,281,512 + RR42_RS15155 0.16 -0.3 3,281,512 + RR42_RS15155 0.16 +0.3 3,281,512 + RR42_RS15155 0.16 +0.4 3,281,512 + RR42_RS15155 0.16 +1.3 3,281,512 + RR42_RS15155 0.16 -0.4 3,281,512 + RR42_RS15155 0.16 -0.7 3,281,515 + RR42_RS15155 0.16 -0.6 3,281,516 - RR42_RS15155 0.16 -0.2 3,281,516 - RR42_RS15155 0.16 +0.4 3,281,626 + RR42_RS15155 0.25 +0.0 3,281,626 + RR42_RS15155 0.25 -0.6 3,281,627 - RR42_RS15155 0.25 -0.4 3,281,627 - RR42_RS15155 0.25 -0.3 3,281,627 - RR42_RS15155 0.25 +0.3 3,281,627 - RR42_RS15155 0.25 -0.5 3,281,627 - RR42_RS15155 0.25 +0.4 3,281,650 + RR42_RS15155 0.27 +0.1 3,281,651 - RR42_RS15155 0.27 +0.4 3,281,662 + RR42_RS15155 0.28 +0.4 3,281,698 + RR42_RS15155 0.31 +0.8 3,281,698 + RR42_RS15155 0.31 -1.8 3,281,698 + RR42_RS15155 0.31 -0.2 3,281,699 - RR42_RS15155 0.31 -0.2 3,281,699 - RR42_RS15155 0.31 -0.6 3,281,699 - RR42_RS15155 0.31 -0.1 3,281,873 - RR42_RS15155 0.45 -0.7 3,281,932 + RR42_RS15155 0.50 -0.3 3,281,933 - RR42_RS15155 0.50 -0.7 3,281,992 + RR42_RS15155 0.55 -0.4 3,281,993 - RR42_RS15155 0.55 -0.5 3,281,993 - RR42_RS15155 0.55 -0.4 3,281,993 - RR42_RS15155 0.55 -0.6 3,282,042 + RR42_RS15155 0.59 +0.6 3,282,043 - RR42_RS15155 0.59 +0.4 3,282,043 - RR42_RS15155 0.59 +0.4 3,282,493 + +0.1 3,282,493 + -0.3 3,282,495 + +1.0 3,282,777 + -0.1 3,282,777 + +0.2 3,282,777 + +0.3 3,282,777 + -0.1 3,282,778 - +0.3 3,282,778 - -0.2 3,282,778 - -0.7 3,282,873 + RR42_RS15160 0.28 +0.1 3,282,873 + RR42_RS15160 0.28 +0.0 3,282,886 - RR42_RS15160 0.31 +0.4 3,282,886 - RR42_RS15160 0.31 -0.8 3,282,886 - RR42_RS15160 0.31 -0.0 3,283,312 - +0.1 3,283,312 - +0.2 3,283,507 + RR42_RS15165 0.25 -0.1 3,283,534 + RR42_RS15165 0.28 +1.8 3,283,534 + RR42_RS15165 0.28 +0.7
Or see this region's nucleotide sequence