Experiment: R2A with Bacitracin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS14920 and RR42_RS14925 are separated by 74 nucleotides RR42_RS14925 and RR42_RS14930 are separated by 52 nucleotides RR42_RS14930 and RR42_RS14935 are separated by 15 nucleotides
RR42_RS14920: RR42_RS14920 - membrane protein, at 3,226,116 to 3,227,891
_RS14920
RR42_RS14925: RR42_RS14925 - hypothetical protein, at 3,227,966 to 3,228,634
_RS14925
RR42_RS14930: RR42_RS14930 - biopolymer transporter ExbD, at 3,228,687 to 3,229,097
_RS14930
RR42_RS14935: RR42_RS14935 - biopolymer transporter ExbB, at 3,229,113 to 3,230,912
_RS14935
Position (kb) 3227
3228
3229 Strain fitness (log2 ratio) -1
0
1
2 at 3227.102 kb on - strand, within RR42_RS14920 at 3227.398 kb on + strand, within RR42_RS14920 at 3227.558 kb on + strand, within RR42_RS14920 at 3227.559 kb on - strand, within RR42_RS14920 at 3227.560 kb on + strand, within RR42_RS14920 at 3227.907 kb on + strand at 3227.907 kb on + strand at 3228.220 kb on - strand, within RR42_RS14925 at 3228.220 kb on - strand, within RR42_RS14925 at 3228.220 kb on - strand, within RR42_RS14925 at 3228.228 kb on + strand, within RR42_RS14925 at 3228.476 kb on - strand, within RR42_RS14925 at 3228.769 kb on + strand, within RR42_RS14930 at 3228.769 kb on + strand, within RR42_RS14930 at 3228.770 kb on - strand, within RR42_RS14930 at 3229.090 kb on + strand at 3229.090 kb on + strand at 3229.244 kb on - strand at 3229.579 kb on + strand, within RR42_RS14935 at 3229.579 kb on + strand, within RR42_RS14935
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Bacitracin 0.5 mg/ml remove 3,227,102 - RR42_RS14920 0.56 +0.6 3,227,398 + RR42_RS14920 0.72 +0.5 3,227,558 + RR42_RS14920 0.81 +0.1 3,227,559 - RR42_RS14920 0.81 +2.3 3,227,560 + RR42_RS14920 0.81 +0.3 3,227,907 + +0.4 3,227,907 + +1.0 3,228,220 - RR42_RS14925 0.38 -0.5 3,228,220 - RR42_RS14925 0.38 +0.7 3,228,220 - RR42_RS14925 0.38 +0.3 3,228,228 + RR42_RS14925 0.39 +0.6 3,228,476 - RR42_RS14925 0.76 -0.6 3,228,769 + RR42_RS14930 0.20 +1.8 3,228,769 + RR42_RS14930 0.20 +0.4 3,228,770 - RR42_RS14930 0.20 +0.8 3,229,090 + +0.9 3,229,090 + +0.6 3,229,244 - +0.3 3,229,579 + RR42_RS14935 0.26 -0.2 3,229,579 + RR42_RS14935 0.26 +0.3
Or see this region's nucleotide sequence