Experiment: R2A with Bacitracin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS14685 and RR42_RS14690 are separated by 556 nucleotides RR42_RS14690 and RR42_RS14695 are separated by 118 nucleotides
RR42_RS14685: RR42_RS14685 - ABC transporter ATP-binding protein, at 3,163,933 to 3,165,855
_RS14685
RR42_RS14690: RR42_RS14690 - amino acid ABC transporter substrate-binding protein, at 3,166,412 to 3,167,338
_RS14690
RR42_RS14695: RR42_RS14695 - membrane protein, at 3,167,457 to 3,168,650
_RS14695
Position (kb)
3166
3167
3168 Strain fitness (log2 ratio)
-2
-1
0
1 at 3165.554 kb on + strand, within RR42_RS14685 at 3165.647 kb on + strand, within RR42_RS14685 at 3165.648 kb on - strand, within RR42_RS14685 at 3165.865 kb on - strand at 3165.997 kb on + strand at 3165.997 kb on + strand at 3165.998 kb on - strand at 3166.132 kb on + strand at 3166.132 kb on + strand at 3166.132 kb on + strand at 3166.132 kb on + strand at 3166.133 kb on - strand at 3166.133 kb on - strand at 3166.133 kb on - strand at 3166.133 kb on - strand at 3166.133 kb on - strand at 3166.155 kb on + strand at 3166.161 kb on + strand at 3166.161 kb on + strand at 3166.162 kb on - strand at 3166.332 kb on + strand at 3166.332 kb on + strand at 3166.333 kb on - strand at 3166.622 kb on + strand, within RR42_RS14690 at 3166.821 kb on - strand, within RR42_RS14690 at 3166.821 kb on - strand, within RR42_RS14690 at 3166.821 kb on - strand, within RR42_RS14690 at 3166.821 kb on - strand, within RR42_RS14690 at 3167.058 kb on - strand, within RR42_RS14690 at 3167.094 kb on - strand, within RR42_RS14690 at 3167.201 kb on + strand, within RR42_RS14690 at 3167.202 kb on - strand, within RR42_RS14690 at 3167.226 kb on - strand, within RR42_RS14690 at 3167.288 kb on + strand at 3167.289 kb on - strand at 3167.289 kb on - strand at 3167.289 kb on - strand at 3167.392 kb on + strand at 3167.456 kb on + strand at 3167.456 kb on + strand at 3167.456 kb on + strand at 3167.456 kb on + strand at 3167.457 kb on - strand at 3167.457 kb on - strand at 3167.457 kb on - strand at 3167.457 kb on - strand at 3167.457 kb on - strand at 3167.457 kb on - strand at 3167.457 kb on - strand at 3167.458 kb on + strand at 3167.459 kb on - strand at 3167.643 kb on - strand, within RR42_RS14695 at 3167.643 kb on - strand, within RR42_RS14695 at 3167.643 kb on - strand, within RR42_RS14695 at 3167.946 kb on + strand, within RR42_RS14695 at 3167.946 kb on + strand, within RR42_RS14695 at 3167.976 kb on + strand, within RR42_RS14695 at 3167.976 kb on + strand, within RR42_RS14695 at 3167.976 kb on + strand, within RR42_RS14695 at 3167.977 kb on - strand, within RR42_RS14695 at 3168.034 kb on + strand, within RR42_RS14695 at 3168.034 kb on + strand, within RR42_RS14695 at 3168.034 kb on + strand, within RR42_RS14695 at 3168.189 kb on + strand, within RR42_RS14695 at 3168.190 kb on - strand, within RR42_RS14695 at 3168.196 kb on + strand, within RR42_RS14695 at 3168.197 kb on - strand, within RR42_RS14695 at 3168.197 kb on - strand, within RR42_RS14695 at 3168.197 kb on - strand, within RR42_RS14695 at 3168.197 kb on - strand, within RR42_RS14695 at 3168.284 kb on + strand, within RR42_RS14695 at 3168.336 kb on + strand, within RR42_RS14695 at 3168.338 kb on + strand, within RR42_RS14695 at 3168.338 kb on + strand, within RR42_RS14695
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Bacitracin 0.5 mg/ml remove 3,165,554 + RR42_RS14685 0.84 +0.6 3,165,647 + RR42_RS14685 0.89 -0.4 3,165,648 - RR42_RS14685 0.89 -0.0 3,165,865 - -0.3 3,165,997 + -0.2 3,165,997 + -0.5 3,165,998 - -0.3 3,166,132 + -1.3 3,166,132 + -0.8 3,166,132 + -0.6 3,166,132 + +0.0 3,166,133 - -0.1 3,166,133 - -1.4 3,166,133 - +0.1 3,166,133 - -0.6 3,166,133 - -1.0 3,166,155 + +0.2 3,166,161 + -0.7 3,166,161 + +0.4 3,166,162 - -0.6 3,166,332 + -0.2 3,166,332 + -0.0 3,166,333 - -0.0 3,166,622 + RR42_RS14690 0.23 +0.6 3,166,821 - RR42_RS14690 0.44 +0.9 3,166,821 - RR42_RS14690 0.44 +0.4 3,166,821 - RR42_RS14690 0.44 -0.2 3,166,821 - RR42_RS14690 0.44 +0.4 3,167,058 - RR42_RS14690 0.70 +0.2 3,167,094 - RR42_RS14690 0.74 +0.1 3,167,201 + RR42_RS14690 0.85 +1.4 3,167,202 - RR42_RS14690 0.85 -0.3 3,167,226 - RR42_RS14690 0.88 -0.8 3,167,288 + +1.2 3,167,289 - +0.7 3,167,289 - -0.3 3,167,289 - -1.7 3,167,392 + -0.9 3,167,456 + -0.4 3,167,456 + -0.7 3,167,456 + -0.4 3,167,456 + +0.1 3,167,457 - -0.3 3,167,457 - +1.6 3,167,457 - -0.1 3,167,457 - +0.3 3,167,457 - -0.7 3,167,457 - +0.5 3,167,457 - -0.2 3,167,458 + -0.2 3,167,459 - -0.4 3,167,643 - RR42_RS14695 0.16 -0.3 3,167,643 - RR42_RS14695 0.16 +0.4 3,167,643 - RR42_RS14695 0.16 -0.0 3,167,946 + RR42_RS14695 0.41 -0.8 3,167,946 + RR42_RS14695 0.41 -0.4 3,167,976 + RR42_RS14695 0.43 -0.0 3,167,976 + RR42_RS14695 0.43 -0.2 3,167,976 + RR42_RS14695 0.43 +0.3 3,167,977 - RR42_RS14695 0.44 +0.7 3,168,034 + RR42_RS14695 0.48 -1.1 3,168,034 + RR42_RS14695 0.48 +0.1 3,168,034 + RR42_RS14695 0.48 -0.7 3,168,189 + RR42_RS14695 0.61 +0.2 3,168,190 - RR42_RS14695 0.61 -1.9 3,168,196 + RR42_RS14695 0.62 -0.2 3,168,197 - RR42_RS14695 0.62 +0.1 3,168,197 - RR42_RS14695 0.62 -0.2 3,168,197 - RR42_RS14695 0.62 +0.2 3,168,197 - RR42_RS14695 0.62 +0.0 3,168,284 + RR42_RS14695 0.69 -0.7 3,168,336 + RR42_RS14695 0.74 +0.5 3,168,338 + RR42_RS14695 0.74 -0.3 3,168,338 + RR42_RS14695 0.74 -0.4
Or see this region's nucleotide sequence