Experiment: R2A with Bacitracin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS13255 and RR42_RS13260 overlap by 4 nucleotides RR42_RS13260 and RR42_RS13265 are separated by 198 nucleotides
RR42_RS13255: RR42_RS13255 - hydroxymethylglutaryl-CoA lyase, at 2,854,720 to 2,855,688
_RS13255
RR42_RS13260: RR42_RS13260 - CoA-transferase, at 2,855,685 to 2,856,881
_RS13260
RR42_RS13265: RR42_RS13265 - GntR family transcriptional regulator, at 2,857,080 to 2,857,985
_RS13265
Position (kb)
2855
2856
2857 Strain fitness (log2 ratio)
-1
0
1
2 at 2854.899 kb on + strand, within RR42_RS13255 at 2854.900 kb on - strand, within RR42_RS13255 at 2854.900 kb on - strand, within RR42_RS13255 at 2854.900 kb on - strand, within RR42_RS13255 at 2854.900 kb on - strand, within RR42_RS13255 at 2854.900 kb on - strand, within RR42_RS13255 at 2854.900 kb on - strand, within RR42_RS13255 at 2855.120 kb on + strand, within RR42_RS13255 at 2855.121 kb on - strand, within RR42_RS13255 at 2855.346 kb on + strand, within RR42_RS13255 at 2855.347 kb on - strand, within RR42_RS13255 at 2855.442 kb on - strand, within RR42_RS13255 at 2855.893 kb on + strand, within RR42_RS13260 at 2855.926 kb on + strand, within RR42_RS13260 at 2855.927 kb on - strand, within RR42_RS13260 at 2856.146 kb on - strand, within RR42_RS13260 at 2856.146 kb on - strand, within RR42_RS13260 at 2856.236 kb on - strand, within RR42_RS13260 at 2856.278 kb on - strand, within RR42_RS13260 at 2856.431 kb on - strand, within RR42_RS13260 at 2856.556 kb on + strand, within RR42_RS13260 at 2856.557 kb on - strand, within RR42_RS13260 at 2856.724 kb on + strand, within RR42_RS13260 at 2856.725 kb on - strand, within RR42_RS13260 at 2856.725 kb on - strand, within RR42_RS13260 at 2856.910 kb on + strand at 2856.911 kb on - strand at 2857.406 kb on - strand, within RR42_RS13265 at 2857.406 kb on - strand, within RR42_RS13265 at 2857.406 kb on - strand, within RR42_RS13265 at 2857.676 kb on + strand, within RR42_RS13265 at 2857.676 kb on + strand, within RR42_RS13265 at 2857.677 kb on - strand, within RR42_RS13265 at 2857.718 kb on - strand, within RR42_RS13265
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Bacitracin 0.5 mg/ml remove 2,854,899 + RR42_RS13255 0.18 +1.0 2,854,900 - RR42_RS13255 0.19 +0.7 2,854,900 - RR42_RS13255 0.19 +1.2 2,854,900 - RR42_RS13255 0.19 +1.1 2,854,900 - RR42_RS13255 0.19 +0.8 2,854,900 - RR42_RS13255 0.19 +1.7 2,854,900 - RR42_RS13255 0.19 +0.4 2,855,120 + RR42_RS13255 0.41 +0.7 2,855,121 - RR42_RS13255 0.41 +1.7 2,855,346 + RR42_RS13255 0.65 +0.7 2,855,347 - RR42_RS13255 0.65 +0.5 2,855,442 - RR42_RS13255 0.75 -0.3 2,855,893 + RR42_RS13260 0.17 +0.4 2,855,926 + RR42_RS13260 0.20 +0.7 2,855,927 - RR42_RS13260 0.20 +0.8 2,856,146 - RR42_RS13260 0.39 +2.2 2,856,146 - RR42_RS13260 0.39 +0.5 2,856,236 - RR42_RS13260 0.46 +0.1 2,856,278 - RR42_RS13260 0.50 +0.9 2,856,431 - RR42_RS13260 0.62 +1.0 2,856,556 + RR42_RS13260 0.73 +0.6 2,856,557 - RR42_RS13260 0.73 +1.0 2,856,724 + RR42_RS13260 0.87 +1.2 2,856,725 - RR42_RS13260 0.87 -0.1 2,856,725 - RR42_RS13260 0.87 +0.7 2,856,910 + +0.8 2,856,911 - +0.7 2,857,406 - RR42_RS13265 0.36 -0.4 2,857,406 - RR42_RS13265 0.36 +0.4 2,857,406 - RR42_RS13265 0.36 +0.7 2,857,676 + RR42_RS13265 0.66 +0.8 2,857,676 + RR42_RS13265 0.66 +0.3 2,857,677 - RR42_RS13265 0.66 +0.5 2,857,718 - RR42_RS13265 0.70 +0.2
Or see this region's nucleotide sequence