Experiment: R2A with Bacitracin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS13245 and RR42_RS13250 are separated by 233 nucleotides RR42_RS13250 and RR42_RS13255 are separated by 221 nucleotides
RR42_RS13245: RR42_RS13245 - ABC transporter substrate-binding protein, at 2,852,268 to 2,853,314
_RS13245
RR42_RS13250: RR42_RS13250 - LacI family transcriptional regulator, at 2,853,548 to 2,854,498
_RS13250
RR42_RS13255: RR42_RS13255 - hydroxymethylglutaryl-CoA lyase, at 2,854,720 to 2,855,688
_RS13255
Position (kb)
2853
2854
2855 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2852.605 kb on + strand, within RR42_RS13245 at 2852.606 kb on - strand, within RR42_RS13245 at 2853.014 kb on + strand, within RR42_RS13245 at 2853.014 kb on + strand, within RR42_RS13245 at 2853.014 kb on + strand, within RR42_RS13245 at 2853.015 kb on - strand, within RR42_RS13245 at 2853.015 kb on - strand, within RR42_RS13245 at 2853.015 kb on - strand, within RR42_RS13245 at 2853.025 kb on + strand, within RR42_RS13245 at 2853.130 kb on + strand, within RR42_RS13245 at 2853.163 kb on + strand, within RR42_RS13245 at 2853.166 kb on + strand, within RR42_RS13245 at 2853.166 kb on + strand, within RR42_RS13245 at 2853.167 kb on - strand, within RR42_RS13245 at 2853.167 kb on - strand, within RR42_RS13245 at 2853.308 kb on + strand at 2853.310 kb on + strand at 2853.310 kb on + strand at 2853.311 kb on - strand at 2853.377 kb on + strand at 2853.377 kb on + strand at 2853.378 kb on - strand at 2853.388 kb on + strand at 2853.388 kb on + strand at 2853.388 kb on + strand at 2853.389 kb on - strand at 2853.389 kb on - strand at 2853.455 kb on + strand at 2853.455 kb on + strand at 2853.456 kb on - strand at 2853.456 kb on - strand at 2853.456 kb on - strand at 2853.456 kb on - strand at 2853.497 kb on + strand at 2853.498 kb on - strand at 2853.523 kb on - strand at 2853.523 kb on - strand at 2853.612 kb on + strand at 2853.757 kb on - strand, within RR42_RS13250 at 2853.757 kb on - strand, within RR42_RS13250 at 2853.757 kb on - strand, within RR42_RS13250 at 2853.968 kb on + strand, within RR42_RS13250 at 2853.970 kb on - strand, within RR42_RS13250 at 2853.970 kb on - strand, within RR42_RS13250 at 2853.970 kb on - strand, within RR42_RS13250 at 2853.970 kb on - strand, within RR42_RS13250 at 2854.056 kb on + strand, within RR42_RS13250 at 2854.087 kb on - strand, within RR42_RS13250 at 2854.087 kb on - strand, within RR42_RS13250 at 2854.258 kb on - strand, within RR42_RS13250 at 2854.328 kb on + strand, within RR42_RS13250 at 2854.328 kb on + strand, within RR42_RS13250 at 2854.328 kb on + strand, within RR42_RS13250 at 2854.328 kb on + strand, within RR42_RS13250 at 2854.899 kb on + strand, within RR42_RS13255 at 2854.900 kb on - strand, within RR42_RS13255 at 2854.900 kb on - strand, within RR42_RS13255 at 2854.900 kb on - strand, within RR42_RS13255 at 2854.900 kb on - strand, within RR42_RS13255 at 2854.900 kb on - strand, within RR42_RS13255 at 2854.900 kb on - strand, within RR42_RS13255 at 2855.120 kb on + strand, within RR42_RS13255 at 2855.121 kb on - strand, within RR42_RS13255 at 2855.346 kb on + strand, within RR42_RS13255 at 2855.347 kb on - strand, within RR42_RS13255 at 2855.442 kb on - strand, within RR42_RS13255
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Bacitracin 0.5 mg/ml remove 2,852,605 + RR42_RS13245 0.32 +0.6 2,852,606 - RR42_RS13245 0.32 +0.4 2,853,014 + RR42_RS13245 0.71 -0.7 2,853,014 + RR42_RS13245 0.71 +1.2 2,853,014 + RR42_RS13245 0.71 +1.4 2,853,015 - RR42_RS13245 0.71 +1.3 2,853,015 - RR42_RS13245 0.71 +0.8 2,853,015 - RR42_RS13245 0.71 +1.1 2,853,025 + RR42_RS13245 0.72 +0.4 2,853,130 + RR42_RS13245 0.82 +0.6 2,853,163 + RR42_RS13245 0.85 +1.3 2,853,166 + RR42_RS13245 0.86 +1.1 2,853,166 + RR42_RS13245 0.86 -0.3 2,853,167 - RR42_RS13245 0.86 +0.1 2,853,167 - RR42_RS13245 0.86 +0.9 2,853,308 + +0.3 2,853,310 + -2.8 2,853,310 + -0.2 2,853,311 - +1.0 2,853,377 + +1.2 2,853,377 + +0.8 2,853,378 - +0.1 2,853,388 + +1.0 2,853,388 + +1.8 2,853,388 + +0.7 2,853,389 - +0.5 2,853,389 - +0.6 2,853,455 + +1.4 2,853,455 + +1.4 2,853,456 - +0.3 2,853,456 - -1.1 2,853,456 - +0.1 2,853,456 - -0.3 2,853,497 + +2.4 2,853,498 - +0.7 2,853,523 - +1.6 2,853,523 - +1.3 2,853,612 + +0.5 2,853,757 - RR42_RS13250 0.22 +0.4 2,853,757 - RR42_RS13250 0.22 -1.1 2,853,757 - RR42_RS13250 0.22 +0.6 2,853,968 + RR42_RS13250 0.44 +1.5 2,853,970 - RR42_RS13250 0.44 -2.4 2,853,970 - RR42_RS13250 0.44 +0.2 2,853,970 - RR42_RS13250 0.44 +1.4 2,853,970 - RR42_RS13250 0.44 +1.2 2,854,056 + RR42_RS13250 0.53 +1.1 2,854,087 - RR42_RS13250 0.57 +0.5 2,854,087 - RR42_RS13250 0.57 +0.5 2,854,258 - RR42_RS13250 0.75 -0.0 2,854,328 + RR42_RS13250 0.82 -2.3 2,854,328 + RR42_RS13250 0.82 +1.7 2,854,328 + RR42_RS13250 0.82 +0.4 2,854,328 + RR42_RS13250 0.82 +1.0 2,854,899 + RR42_RS13255 0.18 +1.0 2,854,900 - RR42_RS13255 0.19 +0.7 2,854,900 - RR42_RS13255 0.19 +1.2 2,854,900 - RR42_RS13255 0.19 +1.1 2,854,900 - RR42_RS13255 0.19 +0.8 2,854,900 - RR42_RS13255 0.19 +1.7 2,854,900 - RR42_RS13255 0.19 +0.4 2,855,120 + RR42_RS13255 0.41 +0.7 2,855,121 - RR42_RS13255 0.41 +1.7 2,855,346 + RR42_RS13255 0.65 +0.7 2,855,347 - RR42_RS13255 0.65 +0.5 2,855,442 - RR42_RS13255 0.75 -0.3
Or see this region's nucleotide sequence