Experiment: R2A with Bacitracin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS13185 and RR42_RS13190 are separated by 6 nucleotides RR42_RS13190 and RR42_RS13195 are separated by 8 nucleotides RR42_RS13195 and RR42_RS13200 are separated by 28 nucleotides
RR42_RS13185: RR42_RS13185 - RNA polymerase sigma factor RpoS, at 2,840,690 to 2,841,868
_RS13185
RR42_RS13190: RR42_RS13190 - outer membrane metallopeptidase lipoprotein nlpD, at 2,841,875 to 2,842,729
_RS13190
RR42_RS13195: RR42_RS13195 - protein-L-isoaspartate O-methyltransferase, at 2,842,738 to 2,843,634
_RS13195
RR42_RS13200: RR42_RS13200 - stationary phase survival protein SurE, at 2,843,663 to 2,844,415
_RS13200
Position (kb)
2841
2842
2843 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2841.249 kb on + strand, within RR42_RS13185 at 2841.249 kb on + strand, within RR42_RS13185 at 2841.250 kb on - strand, within RR42_RS13185 at 2841.250 kb on - strand, within RR42_RS13185 at 2841.262 kb on - strand, within RR42_RS13185 at 2841.357 kb on + strand, within RR42_RS13185 at 2841.358 kb on - strand, within RR42_RS13185 at 2841.502 kb on - strand, within RR42_RS13185 at 2841.502 kb on - strand, within RR42_RS13185 at 2841.502 kb on - strand, within RR42_RS13185 at 2841.502 kb on - strand, within RR42_RS13185 at 2841.748 kb on - strand, within RR42_RS13185 at 2841.892 kb on - strand at 2841.892 kb on - strand at 2841.926 kb on + strand at 2842.050 kb on + strand, within RR42_RS13190 at 2842.050 kb on + strand, within RR42_RS13190 at 2842.050 kb on + strand, within RR42_RS13190 at 2842.050 kb on + strand, within RR42_RS13190 at 2842.051 kb on - strand, within RR42_RS13190 at 2842.051 kb on - strand, within RR42_RS13190 at 2842.051 kb on - strand, within RR42_RS13190 at 2842.054 kb on + strand, within RR42_RS13190 at 2842.054 kb on + strand, within RR42_RS13190 at 2842.055 kb on - strand, within RR42_RS13190 at 2842.055 kb on - strand, within RR42_RS13190 at 2842.055 kb on - strand, within RR42_RS13190 at 2842.549 kb on - strand, within RR42_RS13190 at 2842.721 kb on - strand at 2842.721 kb on - strand at 2842.721 kb on - strand at 2842.721 kb on - strand at 2842.729 kb on + strand at 2842.729 kb on + strand at 2842.729 kb on + strand at 2842.729 kb on + strand at 2842.730 kb on - strand at 2842.730 kb on - strand at 2842.743 kb on + strand at 2842.743 kb on + strand at 2842.744 kb on - strand at 2842.744 kb on - strand at 2842.828 kb on + strand, within RR42_RS13195 at 2842.828 kb on + strand, within RR42_RS13195 at 2842.828 kb on + strand, within RR42_RS13195 at 2842.828 kb on + strand, within RR42_RS13195 at 2842.828 kb on + strand, within RR42_RS13195 at 2842.829 kb on - strand, within RR42_RS13195 at 2842.959 kb on + strand, within RR42_RS13195 at 2843.010 kb on - strand, within RR42_RS13195 at 2843.010 kb on - strand, within RR42_RS13195 at 2843.010 kb on - strand, within RR42_RS13195 at 2843.010 kb on - strand, within RR42_RS13195 at 2843.276 kb on + strand, within RR42_RS13195 at 2843.277 kb on - strand, within RR42_RS13195 at 2843.277 kb on - strand, within RR42_RS13195 at 2843.277 kb on - strand, within RR42_RS13195 at 2843.277 kb on - strand, within RR42_RS13195 at 2843.645 kb on + strand at 2843.648 kb on + strand at 2843.664 kb on + strand at 2843.664 kb on + strand at 2843.664 kb on + strand at 2843.664 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Bacitracin 0.5 mg/ml remove 2,841,249 + RR42_RS13185 0.47 +0.2 2,841,249 + RR42_RS13185 0.47 -0.0 2,841,250 - RR42_RS13185 0.47 +0.1 2,841,250 - RR42_RS13185 0.47 +2.1 2,841,262 - RR42_RS13185 0.49 +0.7 2,841,357 + RR42_RS13185 0.57 -0.8 2,841,358 - RR42_RS13185 0.57 +2.7 2,841,502 - RR42_RS13185 0.69 +1.6 2,841,502 - RR42_RS13185 0.69 -0.2 2,841,502 - RR42_RS13185 0.69 +0.8 2,841,502 - RR42_RS13185 0.69 -0.5 2,841,748 - RR42_RS13185 0.90 +0.9 2,841,892 - -1.7 2,841,892 - -0.2 2,841,926 + -3.6 2,842,050 + RR42_RS13190 0.20 -1.1 2,842,050 + RR42_RS13190 0.20 -0.1 2,842,050 + RR42_RS13190 0.20 -1.2 2,842,050 + RR42_RS13190 0.20 -2.1 2,842,051 - RR42_RS13190 0.21 +0.2 2,842,051 - RR42_RS13190 0.21 -1.7 2,842,051 - RR42_RS13190 0.21 +1.3 2,842,054 + RR42_RS13190 0.21 -2.0 2,842,054 + RR42_RS13190 0.21 -2.3 2,842,055 - RR42_RS13190 0.21 -0.0 2,842,055 - RR42_RS13190 0.21 -2.1 2,842,055 - RR42_RS13190 0.21 -0.7 2,842,549 - RR42_RS13190 0.79 -0.1 2,842,721 - +0.9 2,842,721 - -0.2 2,842,721 - +0.5 2,842,721 - +0.0 2,842,729 + -0.5 2,842,729 + +0.4 2,842,729 + -0.1 2,842,729 + +0.0 2,842,730 - +0.9 2,842,730 - +0.5 2,842,743 + +0.3 2,842,743 + +0.1 2,842,744 - +0.1 2,842,744 - -0.4 2,842,828 + RR42_RS13195 0.10 -0.5 2,842,828 + RR42_RS13195 0.10 +0.6 2,842,828 + RR42_RS13195 0.10 -0.3 2,842,828 + RR42_RS13195 0.10 -0.1 2,842,828 + RR42_RS13195 0.10 -0.7 2,842,829 - RR42_RS13195 0.10 +1.1 2,842,959 + RR42_RS13195 0.25 +0.0 2,843,010 - RR42_RS13195 0.30 +0.3 2,843,010 - RR42_RS13195 0.30 +1.2 2,843,010 - RR42_RS13195 0.30 +0.7 2,843,010 - RR42_RS13195 0.30 +0.1 2,843,276 + RR42_RS13195 0.60 -0.1 2,843,277 - RR42_RS13195 0.60 -0.1 2,843,277 - RR42_RS13195 0.60 +0.2 2,843,277 - RR42_RS13195 0.60 +0.2 2,843,277 - RR42_RS13195 0.60 +0.5 2,843,645 + +0.3 2,843,648 + +0.1 2,843,664 + -0.1 2,843,664 + -0.1 2,843,664 + +0.6 2,843,664 + +1.4
Or see this region's nucleotide sequence