Experiment: R2A with Bacitracin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS12195 and RR42_RS12200 are separated by 423 nucleotides RR42_RS12200 and RR42_RS12205 are separated by 352 nucleotides
RR42_RS12195: RR42_RS12195 - peptidase, at 2,622,490 to 2,623,158
_RS12195
RR42_RS12200: RR42_RS12200 - isocitrate lyase, at 2,623,582 to 2,624,874
_RS12200
RR42_RS12205: RR42_RS12205 - alpha/beta hydrolase, at 2,625,227 to 2,626,189
_RS12205
Position (kb)
2623
2624
2625 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2622.656 kb on + strand, within RR42_RS12195 at 2622.656 kb on + strand, within RR42_RS12195 at 2622.656 kb on + strand, within RR42_RS12195 at 2622.797 kb on + strand, within RR42_RS12195 at 2622.797 kb on + strand, within RR42_RS12195 at 2622.905 kb on + strand, within RR42_RS12195 at 2622.905 kb on + strand, within RR42_RS12195 at 2622.906 kb on - strand, within RR42_RS12195 at 2622.959 kb on + strand, within RR42_RS12195 at 2622.960 kb on - strand, within RR42_RS12195 at 2623.031 kb on + strand, within RR42_RS12195 at 2623.032 kb on - strand, within RR42_RS12195 at 2623.032 kb on - strand, within RR42_RS12195 at 2623.359 kb on + strand at 2623.359 kb on + strand at 2623.388 kb on - strand at 2623.396 kb on + strand at 2623.490 kb on + strand at 2623.491 kb on - strand at 2623.686 kb on - strand at 2623.779 kb on + strand, within RR42_RS12200 at 2623.779 kb on + strand, within RR42_RS12200 at 2623.780 kb on - strand, within RR42_RS12200 at 2623.780 kb on - strand, within RR42_RS12200 at 2623.780 kb on - strand, within RR42_RS12200 at 2623.794 kb on - strand, within RR42_RS12200 at 2623.794 kb on - strand, within RR42_RS12200 at 2623.799 kb on + strand, within RR42_RS12200 at 2623.820 kb on + strand, within RR42_RS12200 at 2623.952 kb on + strand, within RR42_RS12200 at 2623.953 kb on - strand, within RR42_RS12200 at 2623.953 kb on - strand, within RR42_RS12200 at 2623.953 kb on - strand, within RR42_RS12200 at 2624.063 kb on + strand, within RR42_RS12200 at 2624.111 kb on + strand, within RR42_RS12200 at 2624.567 kb on + strand, within RR42_RS12200 at 2624.618 kb on + strand, within RR42_RS12200 at 2624.618 kb on + strand, within RR42_RS12200 at 2624.618 kb on + strand, within RR42_RS12200 at 2624.618 kb on + strand, within RR42_RS12200 at 2624.618 kb on + strand, within RR42_RS12200 at 2624.619 kb on - strand, within RR42_RS12200 at 2624.619 kb on - strand, within RR42_RS12200 at 2624.619 kb on - strand, within RR42_RS12200 at 2624.796 kb on - strand at 2624.796 kb on - strand at 2624.796 kb on - strand at 2624.892 kb on + strand at 2624.914 kb on - strand at 2625.432 kb on + strand, within RR42_RS12205 at 2625.511 kb on + strand, within RR42_RS12205 at 2625.511 kb on + strand, within RR42_RS12205 at 2625.512 kb on - strand, within RR42_RS12205 at 2625.512 kb on - strand, within RR42_RS12205 at 2625.639 kb on - strand, within RR42_RS12205 at 2625.667 kb on + strand, within RR42_RS12205 at 2625.668 kb on - strand, within RR42_RS12205 at 2625.668 kb on - strand, within RR42_RS12205 at 2625.669 kb on + strand, within RR42_RS12205 at 2625.669 kb on + strand, within RR42_RS12205 at 2625.669 kb on + strand, within RR42_RS12205 at 2625.669 kb on + strand, within RR42_RS12205 at 2625.669 kb on + strand, within RR42_RS12205 at 2625.669 kb on + strand, within RR42_RS12205 at 2625.669 kb on + strand, within RR42_RS12205 at 2625.670 kb on - strand, within RR42_RS12205 at 2625.670 kb on - strand, within RR42_RS12205 at 2625.670 kb on - strand, within RR42_RS12205 at 2625.670 kb on - strand, within RR42_RS12205 at 2625.670 kb on - strand, within RR42_RS12205 at 2625.670 kb on - strand, within RR42_RS12205 at 2625.670 kb on - strand, within RR42_RS12205 at 2625.670 kb on - strand, within RR42_RS12205 at 2625.675 kb on + strand, within RR42_RS12205 at 2625.675 kb on + strand, within RR42_RS12205 at 2625.675 kb on + strand, within RR42_RS12205 at 2625.676 kb on - strand, within RR42_RS12205 at 2625.676 kb on - strand, within RR42_RS12205 at 2625.787 kb on + strand, within RR42_RS12205 at 2625.787 kb on + strand, within RR42_RS12205 at 2625.787 kb on + strand, within RR42_RS12205 at 2625.788 kb on - strand, within RR42_RS12205 at 2625.788 kb on - strand, within RR42_RS12205 at 2625.788 kb on - strand, within RR42_RS12205 at 2625.788 kb on - strand, within RR42_RS12205 at 2625.788 kb on - strand, within RR42_RS12205 at 2625.788 kb on - strand, within RR42_RS12205 at 2625.789 kb on + strand, within RR42_RS12205 at 2625.790 kb on - strand, within RR42_RS12205 at 2625.790 kb on - strand, within RR42_RS12205 at 2625.790 kb on - strand, within RR42_RS12205 at 2625.790 kb on - strand, within RR42_RS12205
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Bacitracin 0.5 mg/ml remove 2,622,656 + RR42_RS12195 0.25 -0.2 2,622,656 + RR42_RS12195 0.25 -1.5 2,622,656 + RR42_RS12195 0.25 +0.1 2,622,797 + RR42_RS12195 0.46 +0.7 2,622,797 + RR42_RS12195 0.46 -2.7 2,622,905 + RR42_RS12195 0.62 -1.9 2,622,905 + RR42_RS12195 0.62 +0.1 2,622,906 - RR42_RS12195 0.62 -0.4 2,622,959 + RR42_RS12195 0.70 -0.5 2,622,960 - RR42_RS12195 0.70 +1.4 2,623,031 + RR42_RS12195 0.81 +0.6 2,623,032 - RR42_RS12195 0.81 -1.2 2,623,032 - RR42_RS12195 0.81 -0.0 2,623,359 + +0.8 2,623,359 + +0.4 2,623,388 - -0.4 2,623,396 + -0.5 2,623,490 + +0.3 2,623,491 - -1.0 2,623,686 - +0.8 2,623,779 + RR42_RS12200 0.15 +0.3 2,623,779 + RR42_RS12200 0.15 +0.2 2,623,780 - RR42_RS12200 0.15 +0.2 2,623,780 - RR42_RS12200 0.15 +0.1 2,623,780 - RR42_RS12200 0.15 -0.3 2,623,794 - RR42_RS12200 0.16 -0.6 2,623,794 - RR42_RS12200 0.16 +0.8 2,623,799 + RR42_RS12200 0.17 -0.5 2,623,820 + RR42_RS12200 0.18 -0.0 2,623,952 + RR42_RS12200 0.29 -0.8 2,623,953 - RR42_RS12200 0.29 +1.8 2,623,953 - RR42_RS12200 0.29 -1.7 2,623,953 - RR42_RS12200 0.29 -0.7 2,624,063 + RR42_RS12200 0.37 +0.1 2,624,111 + RR42_RS12200 0.41 -0.2 2,624,567 + RR42_RS12200 0.76 +0.2 2,624,618 + RR42_RS12200 0.80 -0.3 2,624,618 + RR42_RS12200 0.80 +0.6 2,624,618 + RR42_RS12200 0.80 -0.1 2,624,618 + RR42_RS12200 0.80 +0.2 2,624,618 + RR42_RS12200 0.80 +0.7 2,624,619 - RR42_RS12200 0.80 +0.1 2,624,619 - RR42_RS12200 0.80 +0.6 2,624,619 - RR42_RS12200 0.80 +1.4 2,624,796 - +0.3 2,624,796 - -0.7 2,624,796 - -0.0 2,624,892 + -1.3 2,624,914 - -0.3 2,625,432 + RR42_RS12205 0.21 -0.6 2,625,511 + RR42_RS12205 0.29 +0.8 2,625,511 + RR42_RS12205 0.29 -0.7 2,625,512 - RR42_RS12205 0.30 -1.1 2,625,512 - RR42_RS12205 0.30 +0.8 2,625,639 - RR42_RS12205 0.43 -0.3 2,625,667 + RR42_RS12205 0.46 -1.2 2,625,668 - RR42_RS12205 0.46 -1.3 2,625,668 - RR42_RS12205 0.46 +0.2 2,625,669 + RR42_RS12205 0.46 +0.8 2,625,669 + RR42_RS12205 0.46 -0.5 2,625,669 + RR42_RS12205 0.46 -0.4 2,625,669 + RR42_RS12205 0.46 -0.5 2,625,669 + RR42_RS12205 0.46 -0.4 2,625,669 + RR42_RS12205 0.46 -1.2 2,625,669 + RR42_RS12205 0.46 -0.2 2,625,670 - RR42_RS12205 0.46 +0.6 2,625,670 - RR42_RS12205 0.46 -0.7 2,625,670 - RR42_RS12205 0.46 +0.3 2,625,670 - RR42_RS12205 0.46 -1.0 2,625,670 - RR42_RS12205 0.46 -1.6 2,625,670 - RR42_RS12205 0.46 +0.2 2,625,670 - RR42_RS12205 0.46 +0.3 2,625,670 - RR42_RS12205 0.46 -0.8 2,625,675 + RR42_RS12205 0.47 -0.2 2,625,675 + RR42_RS12205 0.47 -1.2 2,625,675 + RR42_RS12205 0.47 +0.2 2,625,676 - RR42_RS12205 0.47 +0.1 2,625,676 - RR42_RS12205 0.47 -1.0 2,625,787 + RR42_RS12205 0.58 -1.2 2,625,787 + RR42_RS12205 0.58 -0.2 2,625,787 + RR42_RS12205 0.58 -0.9 2,625,788 - RR42_RS12205 0.58 -0.4 2,625,788 - RR42_RS12205 0.58 -0.3 2,625,788 - RR42_RS12205 0.58 +0.0 2,625,788 - RR42_RS12205 0.58 -0.3 2,625,788 - RR42_RS12205 0.58 -1.5 2,625,788 - RR42_RS12205 0.58 -0.2 2,625,789 + RR42_RS12205 0.58 -0.0 2,625,790 - RR42_RS12205 0.58 +0.0 2,625,790 - RR42_RS12205 0.58 -0.4 2,625,790 - RR42_RS12205 0.58 -0.3 2,625,790 - RR42_RS12205 0.58 +2.4
Or see this region's nucleotide sequence