Experiment: R2A with Bacitracin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS12185 and RR42_RS12190 are separated by 123 nucleotides RR42_RS12190 and RR42_RS12195 are separated by 100 nucleotides
RR42_RS12185: RR42_RS12185 - LysR family transcriptional regulator, at 2,620,878 to 2,621,831
_RS12185
RR42_RS12190: RR42_RS12190 - universal stress protein UspA, at 2,621,955 to 2,622,389
_RS12190
RR42_RS12195: RR42_RS12195 - peptidase, at 2,622,490 to 2,623,158
_RS12195
Position (kb)
2621
2622
2623 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2621.389 kb on - strand, within RR42_RS12185 at 2621.754 kb on + strand at 2621.754 kb on + strand at 2621.754 kb on + strand at 2621.754 kb on + strand at 2621.754 kb on + strand at 2621.755 kb on - strand at 2621.755 kb on - strand at 2621.830 kb on - strand at 2621.874 kb on + strand at 2621.874 kb on + strand at 2621.875 kb on - strand at 2621.875 kb on - strand at 2621.875 kb on - strand at 2621.875 kb on - strand at 2622.060 kb on + strand, within RR42_RS12190 at 2622.060 kb on + strand, within RR42_RS12190 at 2622.061 kb on - strand, within RR42_RS12190 at 2622.078 kb on + strand, within RR42_RS12190 at 2622.084 kb on + strand, within RR42_RS12190 at 2622.085 kb on - strand, within RR42_RS12190 at 2622.085 kb on - strand, within RR42_RS12190 at 2622.085 kb on - strand, within RR42_RS12190 at 2622.157 kb on + strand, within RR42_RS12190 at 2622.158 kb on - strand, within RR42_RS12190 at 2622.158 kb on - strand, within RR42_RS12190 at 2622.159 kb on + strand, within RR42_RS12190 at 2622.381 kb on + strand at 2622.381 kb on + strand at 2622.656 kb on + strand, within RR42_RS12195 at 2622.656 kb on + strand, within RR42_RS12195 at 2622.656 kb on + strand, within RR42_RS12195 at 2622.797 kb on + strand, within RR42_RS12195 at 2622.797 kb on + strand, within RR42_RS12195 at 2622.905 kb on + strand, within RR42_RS12195 at 2622.905 kb on + strand, within RR42_RS12195 at 2622.906 kb on - strand, within RR42_RS12195 at 2622.959 kb on + strand, within RR42_RS12195 at 2622.960 kb on - strand, within RR42_RS12195 at 2623.031 kb on + strand, within RR42_RS12195 at 2623.032 kb on - strand, within RR42_RS12195 at 2623.032 kb on - strand, within RR42_RS12195 at 2623.359 kb on + strand at 2623.359 kb on + strand at 2623.388 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Bacitracin 0.5 mg/ml remove 2,621,389 - RR42_RS12185 0.54 +0.0 2,621,754 + +0.3 2,621,754 + -0.6 2,621,754 + -0.1 2,621,754 + +0.5 2,621,754 + +1.0 2,621,755 - +0.4 2,621,755 - -0.5 2,621,830 - -0.4 2,621,874 + -0.3 2,621,874 + +2.4 2,621,875 - +1.2 2,621,875 - +0.1 2,621,875 - -2.0 2,621,875 - -1.0 2,622,060 + RR42_RS12190 0.24 -0.1 2,622,060 + RR42_RS12190 0.24 +0.7 2,622,061 - RR42_RS12190 0.24 +0.8 2,622,078 + RR42_RS12190 0.28 +0.1 2,622,084 + RR42_RS12190 0.30 +0.4 2,622,085 - RR42_RS12190 0.30 -0.3 2,622,085 - RR42_RS12190 0.30 -0.1 2,622,085 - RR42_RS12190 0.30 +0.0 2,622,157 + RR42_RS12190 0.46 -0.6 2,622,158 - RR42_RS12190 0.47 +0.1 2,622,158 - RR42_RS12190 0.47 +0.4 2,622,159 + RR42_RS12190 0.47 -0.9 2,622,381 + -0.8 2,622,381 + +0.0 2,622,656 + RR42_RS12195 0.25 -0.2 2,622,656 + RR42_RS12195 0.25 -1.5 2,622,656 + RR42_RS12195 0.25 +0.1 2,622,797 + RR42_RS12195 0.46 +0.7 2,622,797 + RR42_RS12195 0.46 -2.7 2,622,905 + RR42_RS12195 0.62 -1.9 2,622,905 + RR42_RS12195 0.62 +0.1 2,622,906 - RR42_RS12195 0.62 -0.4 2,622,959 + RR42_RS12195 0.70 -0.5 2,622,960 - RR42_RS12195 0.70 +1.4 2,623,031 + RR42_RS12195 0.81 +0.6 2,623,032 - RR42_RS12195 0.81 -1.2 2,623,032 - RR42_RS12195 0.81 -0.0 2,623,359 + +0.8 2,623,359 + +0.4 2,623,388 - -0.4
Or see this region's nucleotide sequence