Experiment: R2A with Bacitracin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS11010 and RR42_RS11015 are separated by 141 nucleotides RR42_RS11015 and RR42_RS11020 overlap by 4 nucleotides RR42_RS11020 and RR42_RS11025 are separated by 86 nucleotides RR42_RS11025 and RR42_RS11030 are separated by 141 nucleotides
RR42_RS11010: RR42_RS11010 - macro domain-containing protein, at 2,351,556 to 2,352,077
_RS11010
RR42_RS11015: RR42_RS11015 - hypothetical protein, at 2,352,219 to 2,352,500
_RS11015
RR42_RS11020: RR42_RS11020 - hypothetical protein, at 2,352,497 to 2,352,823
_RS11020
RR42_RS11025: RR42_RS11025 - aspartate racemase, at 2,352,910 to 2,353,620
_RS11025
RR42_RS11030: RR42_RS11030 - alpha/beta hydrolase, at 2,353,762 to 2,354,682
_RS11030
Position (kb)
2352
2353
2354 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2351.918 kb on + strand, within RR42_RS11010 at 2351.919 kb on - strand, within RR42_RS11010 at 2351.934 kb on + strand, within RR42_RS11010 at 2351.934 kb on + strand, within RR42_RS11010 at 2351.934 kb on + strand, within RR42_RS11010 at 2351.935 kb on - strand, within RR42_RS11010 at 2351.935 kb on - strand, within RR42_RS11010 at 2351.964 kb on - strand, within RR42_RS11010 at 2351.964 kb on - strand, within RR42_RS11010 at 2352.212 kb on - strand at 2352.216 kb on + strand at 2352.216 kb on + strand at 2352.216 kb on + strand at 2352.216 kb on + strand at 2352.526 kb on - strand at 2352.526 kb on - strand at 2352.526 kb on - strand at 2352.593 kb on + strand, within RR42_RS11020 at 2352.593 kb on + strand, within RR42_RS11020 at 2352.594 kb on - strand, within RR42_RS11020 at 2352.594 kb on - strand, within RR42_RS11020 at 2352.597 kb on + strand, within RR42_RS11020 at 2352.597 kb on + strand, within RR42_RS11020 at 2352.598 kb on - strand, within RR42_RS11020 at 2352.624 kb on + strand, within RR42_RS11020 at 2352.625 kb on - strand, within RR42_RS11020 at 2352.636 kb on + strand, within RR42_RS11020 at 2352.636 kb on + strand, within RR42_RS11020 at 2352.636 kb on + strand, within RR42_RS11020 at 2352.636 kb on + strand, within RR42_RS11020 at 2352.637 kb on - strand, within RR42_RS11020 at 2352.637 kb on - strand, within RR42_RS11020 at 2352.637 kb on - strand, within RR42_RS11020 at 2352.815 kb on + strand at 2352.911 kb on + strand at 2352.911 kb on + strand at 2353.126 kb on + strand, within RR42_RS11025 at 2353.127 kb on - strand, within RR42_RS11025 at 2353.127 kb on - strand, within RR42_RS11025 at 2353.136 kb on + strand, within RR42_RS11025 at 2353.136 kb on + strand, within RR42_RS11025 at 2353.220 kb on + strand, within RR42_RS11025 at 2353.409 kb on - strand, within RR42_RS11025 at 2353.409 kb on - strand, within RR42_RS11025 at 2353.409 kb on - strand, within RR42_RS11025 at 2353.565 kb on + strand at 2353.566 kb on - strand at 2353.566 kb on - strand at 2353.568 kb on + strand at 2353.665 kb on - strand at 2353.665 kb on - strand at 2353.665 kb on - strand at 2353.974 kb on + strand, within RR42_RS11030 at 2354.027 kb on + strand, within RR42_RS11030 at 2354.028 kb on - strand, within RR42_RS11030 at 2354.091 kb on - strand, within RR42_RS11030 at 2354.222 kb on + strand, within RR42_RS11030 at 2354.223 kb on - strand, within RR42_RS11030 at 2354.276 kb on + strand, within RR42_RS11030 at 2354.276 kb on + strand, within RR42_RS11030 at 2354.276 kb on + strand, within RR42_RS11030 at 2354.276 kb on + strand, within RR42_RS11030 at 2354.276 kb on + strand, within RR42_RS11030 at 2354.276 kb on + strand, within RR42_RS11030 at 2354.276 kb on + strand, within RR42_RS11030 at 2354.277 kb on - strand, within RR42_RS11030 at 2354.277 kb on - strand, within RR42_RS11030 at 2354.277 kb on - strand, within RR42_RS11030 at 2354.277 kb on - strand, within RR42_RS11030 at 2354.277 kb on - strand, within RR42_RS11030 at 2354.391 kb on - strand, within RR42_RS11030 at 2354.616 kb on - strand at 2354.616 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Bacitracin 0.5 mg/ml remove 2,351,918 + RR42_RS11010 0.69 +0.9 2,351,919 - RR42_RS11010 0.70 +2.3 2,351,934 + RR42_RS11010 0.72 -0.4 2,351,934 + RR42_RS11010 0.72 +0.3 2,351,934 + RR42_RS11010 0.72 +0.0 2,351,935 - RR42_RS11010 0.73 -0.2 2,351,935 - RR42_RS11010 0.73 -2.1 2,351,964 - RR42_RS11010 0.78 +0.5 2,351,964 - RR42_RS11010 0.78 -0.5 2,352,212 - -0.5 2,352,216 + -0.6 2,352,216 + +0.2 2,352,216 + -0.7 2,352,216 + -0.3 2,352,526 - -0.5 2,352,526 - -1.5 2,352,526 - +0.3 2,352,593 + RR42_RS11020 0.29 -0.4 2,352,593 + RR42_RS11020 0.29 -0.4 2,352,594 - RR42_RS11020 0.30 +0.1 2,352,594 - RR42_RS11020 0.30 -0.7 2,352,597 + RR42_RS11020 0.31 -0.3 2,352,597 + RR42_RS11020 0.31 +0.2 2,352,598 - RR42_RS11020 0.31 -0.8 2,352,624 + RR42_RS11020 0.39 +0.9 2,352,625 - RR42_RS11020 0.39 -0.4 2,352,636 + RR42_RS11020 0.43 -0.6 2,352,636 + RR42_RS11020 0.43 +0.1 2,352,636 + RR42_RS11020 0.43 +0.2 2,352,636 + RR42_RS11020 0.43 -0.2 2,352,637 - RR42_RS11020 0.43 -1.1 2,352,637 - RR42_RS11020 0.43 +0.2 2,352,637 - RR42_RS11020 0.43 +0.5 2,352,815 + -0.3 2,352,911 + -0.1 2,352,911 + +0.6 2,353,126 + RR42_RS11025 0.30 -0.6 2,353,127 - RR42_RS11025 0.31 -0.5 2,353,127 - RR42_RS11025 0.31 -0.8 2,353,136 + RR42_RS11025 0.32 -0.9 2,353,136 + RR42_RS11025 0.32 +0.2 2,353,220 + RR42_RS11025 0.44 -0.3 2,353,409 - RR42_RS11025 0.70 +0.4 2,353,409 - RR42_RS11025 0.70 -0.8 2,353,409 - RR42_RS11025 0.70 -0.3 2,353,565 + +0.6 2,353,566 - +0.4 2,353,566 - +1.0 2,353,568 + -1.5 2,353,665 - -0.4 2,353,665 - +0.5 2,353,665 - -0.4 2,353,974 + RR42_RS11030 0.23 +0.6 2,354,027 + RR42_RS11030 0.29 -1.3 2,354,028 - RR42_RS11030 0.29 -0.0 2,354,091 - RR42_RS11030 0.36 +0.3 2,354,222 + RR42_RS11030 0.50 +0.5 2,354,223 - RR42_RS11030 0.50 -0.9 2,354,276 + RR42_RS11030 0.56 -0.3 2,354,276 + RR42_RS11030 0.56 +0.4 2,354,276 + RR42_RS11030 0.56 +0.2 2,354,276 + RR42_RS11030 0.56 +0.3 2,354,276 + RR42_RS11030 0.56 +0.3 2,354,276 + RR42_RS11030 0.56 -0.2 2,354,276 + RR42_RS11030 0.56 +0.7 2,354,277 - RR42_RS11030 0.56 -0.3 2,354,277 - RR42_RS11030 0.56 -0.6 2,354,277 - RR42_RS11030 0.56 -1.4 2,354,277 - RR42_RS11030 0.56 -0.7 2,354,277 - RR42_RS11030 0.56 +0.5 2,354,391 - RR42_RS11030 0.68 -0.3 2,354,616 - +0.7 2,354,616 - -0.4
Or see this region's nucleotide sequence