Experiment: R2A with Bacitracin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS09535 and RR42_RS09540 are separated by 260 nucleotides RR42_RS09540 and RR42_RS09545 are separated by 34 nucleotides RR42_RS09545 and RR42_RS09550 are separated by 109 nucleotides RR42_RS09550 and RR42_RS09555 are separated by 276 nucleotides
RR42_RS09535: RR42_RS09535 - AraC family transcriptional regulator, at 2,020,976 to 2,022,025
_RS09535
RR42_RS09540: RR42_RS09540 - twin-arginine translocation pathway signal protein, at 2,022,286 to 2,022,846
_RS09540
RR42_RS09545: RR42_RS09545 - peptidase, at 2,022,881 to 2,024,464
_RS09545
RR42_RS09550: RR42_RS09550 - universal stress protein UspA, at 2,024,574 to 2,025,029
_RS09550
RR42_RS09555: RR42_RS09555 - universal stress protein UspA, at 2,025,306 to 2,025,806
_RS09555
Position (kb)
2022
2023
2024
2025 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2021.914 kb on - strand, within RR42_RS09535 at 2022.170 kb on - strand at 2022.185 kb on + strand at 2022.185 kb on + strand at 2022.287 kb on + strand at 2022.338 kb on + strand at 2022.339 kb on - strand at 2022.339 kb on - strand at 2022.339 kb on - strand at 2022.435 kb on + strand, within RR42_RS09540 at 2022.435 kb on + strand, within RR42_RS09540 at 2022.436 kb on - strand, within RR42_RS09540 at 2022.445 kb on - strand, within RR42_RS09540 at 2022.445 kb on - strand, within RR42_RS09540 at 2022.575 kb on - strand, within RR42_RS09540 at 2022.674 kb on + strand, within RR42_RS09540 at 2022.675 kb on - strand, within RR42_RS09540 at 2022.882 kb on + strand at 2022.883 kb on - strand at 2022.883 kb on - strand at 2022.969 kb on + strand at 2022.969 kb on + strand at 2022.970 kb on - strand at 2022.970 kb on - strand at 2022.970 kb on - strand at 2022.973 kb on + strand at 2023.024 kb on - strand at 2023.233 kb on + strand, within RR42_RS09545 at 2023.233 kb on + strand, within RR42_RS09545 at 2023.234 kb on - strand, within RR42_RS09545 at 2023.234 kb on - strand, within RR42_RS09545 at 2023.305 kb on - strand, within RR42_RS09545 at 2023.305 kb on - strand, within RR42_RS09545 at 2023.473 kb on + strand, within RR42_RS09545 at 2023.526 kb on - strand, within RR42_RS09545 at 2023.812 kb on + strand, within RR42_RS09545 at 2023.813 kb on - strand, within RR42_RS09545 at 2023.813 kb on - strand, within RR42_RS09545 at 2023.890 kb on + strand, within RR42_RS09545 at 2023.891 kb on - strand, within RR42_RS09545 at 2024.166 kb on + strand, within RR42_RS09545 at 2024.167 kb on - strand, within RR42_RS09545 at 2024.878 kb on + strand, within RR42_RS09550 at 2024.878 kb on + strand, within RR42_RS09550 at 2024.878 kb on + strand, within RR42_RS09550 at 2024.878 kb on + strand, within RR42_RS09550 at 2024.878 kb on + strand, within RR42_RS09550 at 2024.878 kb on + strand, within RR42_RS09550 at 2024.879 kb on - strand, within RR42_RS09550 at 2024.900 kb on + strand, within RR42_RS09550 at 2024.920 kb on + strand, within RR42_RS09550 at 2024.920 kb on + strand, within RR42_RS09550 at 2024.920 kb on + strand, within RR42_RS09550 at 2024.931 kb on + strand, within RR42_RS09550 at 2024.932 kb on - strand, within RR42_RS09550 at 2024.932 kb on - strand, within RR42_RS09550 at 2024.980 kb on - strand, within RR42_RS09550 at 2024.980 kb on - strand, within RR42_RS09550 at 2025.016 kb on - strand at 2025.016 kb on - strand at 2025.025 kb on + strand at 2025.025 kb on + strand at 2025.025 kb on + strand at 2025.073 kb on + strand at 2025.111 kb on + strand at 2025.112 kb on - strand at 2025.112 kb on - strand at 2025.112 kb on - strand at 2025.132 kb on + strand at 2025.132 kb on + strand at 2025.133 kb on - strand at 2025.133 kb on - strand at 2025.133 kb on - strand at 2025.218 kb on - strand at 2025.244 kb on + strand at 2025.300 kb on + strand at 2025.300 kb on + strand at 2025.301 kb on - strand at 2025.309 kb on + strand at 2025.309 kb on + strand at 2025.310 kb on - strand at 2025.432 kb on + strand, within RR42_RS09555 at 2025.432 kb on + strand, within RR42_RS09555 at 2025.432 kb on + strand, within RR42_RS09555
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Bacitracin 0.5 mg/ml remove 2,021,914 - RR42_RS09535 0.89 -0.1 2,022,170 - -0.6 2,022,185 + -0.5 2,022,185 + +0.0 2,022,287 + +0.4 2,022,338 + -0.3 2,022,339 - +0.4 2,022,339 - +0.0 2,022,339 - -0.1 2,022,435 + RR42_RS09540 0.27 +0.3 2,022,435 + RR42_RS09540 0.27 -0.1 2,022,436 - RR42_RS09540 0.27 -0.0 2,022,445 - RR42_RS09540 0.28 -0.4 2,022,445 - RR42_RS09540 0.28 -0.6 2,022,575 - RR42_RS09540 0.52 -0.3 2,022,674 + RR42_RS09540 0.69 -0.9 2,022,675 - RR42_RS09540 0.69 -0.4 2,022,882 + +0.3 2,022,883 - -1.4 2,022,883 - +0.3 2,022,969 + -0.1 2,022,969 + +0.7 2,022,970 - -0.6 2,022,970 - -0.7 2,022,970 - +0.0 2,022,973 + -0.2 2,023,024 - +0.2 2,023,233 + RR42_RS09545 0.22 -0.7 2,023,233 + RR42_RS09545 0.22 -0.2 2,023,234 - RR42_RS09545 0.22 +0.4 2,023,234 - RR42_RS09545 0.22 -0.3 2,023,305 - RR42_RS09545 0.27 -0.2 2,023,305 - RR42_RS09545 0.27 +0.0 2,023,473 + RR42_RS09545 0.37 +0.9 2,023,526 - RR42_RS09545 0.41 -1.1 2,023,812 + RR42_RS09545 0.59 -0.5 2,023,813 - RR42_RS09545 0.59 +0.2 2,023,813 - RR42_RS09545 0.59 +0.7 2,023,890 + RR42_RS09545 0.64 -0.8 2,023,891 - RR42_RS09545 0.64 -0.1 2,024,166 + RR42_RS09545 0.81 -0.7 2,024,167 - RR42_RS09545 0.81 -1.1 2,024,878 + RR42_RS09550 0.67 -0.9 2,024,878 + RR42_RS09550 0.67 -0.2 2,024,878 + RR42_RS09550 0.67 -0.3 2,024,878 + RR42_RS09550 0.67 -1.8 2,024,878 + RR42_RS09550 0.67 +0.5 2,024,878 + RR42_RS09550 0.67 -0.1 2,024,879 - RR42_RS09550 0.67 -1.0 2,024,900 + RR42_RS09550 0.71 +0.2 2,024,920 + RR42_RS09550 0.76 +0.0 2,024,920 + RR42_RS09550 0.76 -0.5 2,024,920 + RR42_RS09550 0.76 +0.5 2,024,931 + RR42_RS09550 0.78 +0.5 2,024,932 - RR42_RS09550 0.79 +0.5 2,024,932 - RR42_RS09550 0.79 +0.4 2,024,980 - RR42_RS09550 0.89 -0.4 2,024,980 - RR42_RS09550 0.89 -0.3 2,025,016 - -0.4 2,025,016 - -0.6 2,025,025 + -0.7 2,025,025 + -1.1 2,025,025 + -0.4 2,025,073 + -0.4 2,025,111 + -1.1 2,025,112 - -0.5 2,025,112 - +0.3 2,025,112 - +0.6 2,025,132 + +0.2 2,025,132 + -2.8 2,025,133 - +0.2 2,025,133 - -0.8 2,025,133 - +0.6 2,025,218 - -0.4 2,025,244 + -0.1 2,025,300 + +0.3 2,025,300 + +0.9 2,025,301 - -0.7 2,025,309 + -0.5 2,025,309 + +1.0 2,025,310 - -0.1 2,025,432 + RR42_RS09555 0.25 +0.4 2,025,432 + RR42_RS09555 0.25 +0.4 2,025,432 + RR42_RS09555 0.25 -1.4
Or see this region's nucleotide sequence