Experiment: R2A with Bacitracin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS08785 and RR42_RS08790 are separated by 157 nucleotides RR42_RS08790 and RR42_RS08795 are separated by 50 nucleotides RR42_RS08795 and RR42_RS08800 are separated by 140 nucleotides RR42_RS08800 and RR42_RS08805 are separated by 52 nucleotides
RR42_RS08785: RR42_RS08785 - lipid carrier protein, at 1,841,740 to 1,842,192
_RS08785
RR42_RS08790: RR42_RS08790 - zinc-binding protein, at 1,842,350 to 1,842,799
_RS08790
RR42_RS08795: RR42_RS08795 - molybdopterin-guanine dinucleotide biosynthesis protein B, at 1,842,850 to 1,843,428
_RS08795
RR42_RS08800: RR42_RS08800 - peptidylprolyl isomerase, at 1,843,569 to 1,844,315
_RS08800
RR42_RS08805: RR42_RS08805 - nitrate reductase, at 1,844,368 to 1,845,051
_RS08805
Position (kb) 1842
1843
1844 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 1842.104 kb on - strand, within RR42_RS08785 at 1842.234 kb on + strand at 1842.235 kb on - strand at 1842.235 kb on - strand at 1842.235 kb on - strand at 1842.300 kb on + strand at 1842.390 kb on - strand at 1842.433 kb on + strand, within RR42_RS08790 at 1842.762 kb on + strand at 1842.763 kb on - strand at 1842.763 kb on - strand at 1842.805 kb on + strand at 1842.805 kb on + strand at 1842.830 kb on - strand at 1842.954 kb on + strand, within RR42_RS08795 at 1843.059 kb on - strand, within RR42_RS08795 at 1843.136 kb on - strand, within RR42_RS08795 at 1843.136 kb on - strand, within RR42_RS08795 at 1843.311 kb on + strand, within RR42_RS08795 at 1843.769 kb on - strand, within RR42_RS08800 at 1844.250 kb on - strand at 1844.305 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Bacitracin 0.5 mg/ml remove 1,842,104 - RR42_RS08785 0.80 -0.3 1,842,234 + +0.1 1,842,235 - -0.0 1,842,235 - +0.9 1,842,235 - +0.1 1,842,300 + -0.3 1,842,390 - +0.4 1,842,433 + RR42_RS08790 0.18 +0.3 1,842,762 + -0.9 1,842,763 - -0.7 1,842,763 - +2.8 1,842,805 + -0.6 1,842,805 + -0.5 1,842,830 - +0.0 1,842,954 + RR42_RS08795 0.18 -2.0 1,843,059 - RR42_RS08795 0.36 -0.7 1,843,136 - RR42_RS08795 0.49 -1.4 1,843,136 - RR42_RS08795 0.49 -0.1 1,843,311 + RR42_RS08795 0.80 -1.3 1,843,769 - RR42_RS08800 0.27 -1.5 1,844,250 - +0.6 1,844,305 - +0.6
Or see this region's nucleotide sequence